SNX5 Antibody

Datasheet

Code	CSB-PA872015LA01HU
Size	US$166
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Image	IHC image of CSB-PA872015LA01HU diluted at 1:640 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA872015LA01HU at 1:213, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Western Blot Positive WB detected in: A549 whole cell lysate, 293T whole cell lysate, HepG2 whole cell lysate, Hela whole cell lysate All lanes: SNX5 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 47, 14 kDa Observed band size: 47 kDa
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) SNX5 Polyclonal antibody

Uniprot No.

Q9Y5X3

Target Names

SNX5

Alternative Names

0910001N05Rik antibody; 1810032P22Rik antibody; AU019504 antibody; D2Ertd52e antibody; FLJ10931 antibody; OTTHUMP00000030340 antibody; OTTHUMP00000062927 antibody; OTTMUSP00000003367 antibody; OTTMUSP00000003368 antibody; RP11-504H3.2 antibody; RP23-35E16.2 antibody; snoRNA MBI-43 antibody; SNX 5 antibody; SNX5 antibody; SNX5_HUMAN antibody; Sorting nexin 5 antibody; Sorting nexin-5 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Sorting nexin-5 protein (7-245AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The SNX5 Antibody (Product code: CSB-PA872015LA01HU) is Non-conjugated. For SNX5 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA872015LB01HU	SNX5 Antibody, HRP conjugated	ELISA
FITC	CSB-PA872015LC01HU	SNX5 Antibody, FITC conjugated
Biotin	CSB-PA872015LD01HU	SNX5 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:500-1:1000
IF	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Involved in several stages of intracellular trafficking. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,4-bisphosphate (PtdIns(3,4)P2). Acts in part as component of the retromer membrane-deforming SNX-BAR subcomplex. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX-BAR subcomplex functions to deform the donor membrane into a tubular profile called endosome-to-TGN transport carrier (ETC) (Probable). Does not have in vitro vesicle-to-membrane remodeling activity. Involved in retrograde transport of lysosomal enzyme receptor IGF2R. May function as link between endosomal transport vesicles and dynactin (Probable). Plays a role in the internalization of EGFR after EGF stimulation (Probable). Involved in EGFR endosomal sorting and degradation; the function involves PIP5K1C isoform 3 and is retromer-independent. Together with PIP5K1C isoform 3 facilitates HGS interaction with ubiquitinated EGFR, which initiates EGFR sorting to intraluminal vesicles (ILVs) of the multivesicular body for subsequent lysosomal degradation (Probable). Involved in E-cadherin sorting and degradation; inhibits PIP5K1C isoform 3-mediated E-cadherin degradation. Plays a role in macropinocytosis.

Gene References into Functions

High SNX5 expression is associated with alphavirus infections. PMID: 29743363
sorting nexin 5 (SNX5), a component of the retromer and part of the retrograde transport pathway, interacts with UL35 proteins. binding of UL35 proteins to SNX5 was required for efficient viral replication and for transport of the most abundant cytomegalovirus glycoprotein B to the cytoplasmic viral assembly center. PMID: 29444945
The Chlamydia trachomatis IncE binding site is unique to human SNX5 and related family members SNX6 and SNX32. PMID: 28226239
Sorting nexin 5 and dopamine d1 receptor regulate the expression of the insulin receptor in human renal proximal tubule cells PMID: 25825816
internalization of agonist-activated D1R is regulated by both SNX5 and GRK4, and that SNX5 is critical to the recycling of the receptor to the plasma membrane PMID: 23195037
Data indicate induction of caspase-2 by sorting nexin 5 (SNX5) in papillary thyroid carcinoma PMID: 22486813
SNX5 is localized to a subdomain of the early endosomes and is recruited to the cell membrane following EGF stimulation. PMID: 15561769
SNX5 and SNX6 may constitute functional equivalents of Vps17p in mammalian retromer PMID: 17148574
DOCK180 regulates CI-MPR trafficking via SNX5 and this function is independent of its guanine nucleotide exchange factor activity toward Rac1 PMID: 18596235

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Subcellular Location

Endosome. Early endosome. Early endosome membrane; Peripheral membrane protein; Cytoplasmic side. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm. Cell projection, phagocytic cup. Cell projection, ruffle.

Protein Families

Sorting nexin family

Database Links

HGNC: 14969

OMIM: 605937

KEGG: hsa:27131

STRING: 9606.ENSP00000366988

UniGene: Hs.316890

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Endocytosis

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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