SUMF2 Antibody

Code CSB-PA839844LA01HU
Size US$166
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  • Western Blot
    Positive WB detected in: A549 whole cell lysate, HepG2 whole cell lysate, Jurkat whole cell lysate, K562 whole cell lysate, U87 whole cell lysate, Hela whole cell lysate
    All lanes: SUMF2 antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 34, 25, 38, 24, 35 kDa
    Observed band size: 34 kDa

  • Immunoprecipitating SUMF2 in Hela whole cell lysate
    Lane 1: Rabbit control IgG instead of CSB-PA839844LA01HU in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000)
    Lane 2: CSB-PA839844LA01HU (5ug) + Hela whole cell lysate (500ug)
    Lane 3: Hela whole cell lysate (10ug)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) SUMF2 Polyclonal antibody
Uniprot No.
Target Names
SUMF2
Alternative Names
SUMF2 antibody; PSEC0171 antibody; UNQ1968/PRO4500 antibody; Inactive C-alpha-formylglycine-generating enzyme 2 antibody; Paralog of formylglycine-generating enzyme antibody; pFGE antibody; Sulfatase-modifying factor 2 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Sulfatase-modifying factor 2 protein (26-301AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The SUMF2 Antibody (Product code: CSB-PA839844LA01HU) is Non-conjugated. For SUMF2 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA839844LB01HU SUMF2 Antibody, HRP conjugated ELISA
FITC CSB-PA839844LC01HU SUMF2 Antibody, FITC conjugated
Biotin CSB-PA839844LD01HU SUMF2 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Antigen Affinity purified & Affinity purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IP
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IP 1:1000-1:2000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Lacks formylglycine generating activity and is unable to convert newly synthesized inactive sulfatases to their active form. Inhibits the activation of sulfatases by SUMF1.
Gene References into Functions
  1. SUMF2 interacted with IL-13 and inhibited IL-13 secretion in bronchial smooth muscle cells and lymphocytes, which was independent of IL-13 glycosylation PMID: 19739097
  2. structural analysis of human pFGE by X-ray crystallography [pFGE] PMID: 15687489
  3. pFGE (SUMF2) is the paralog of the Calpha-formylglycine-generating enzyme PMID: 15708861
  4. SUMF2 interacts with sulphatase-modifying factor 1 to regulate sulphatase activities. PMID: 15962010
  5. pFGE is retained in the ER through its C-terminal tetrapeptide PGEL, a noncanonical variant of the classic KDEL ER-retention signal. PMID: 18266766

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Subcellular Location
Endoplasmic reticulum lumen.
Protein Families
Sulfatase-modifying factor family
Tissue Specificity
Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in kidney, liver and placenta.
Database Links

HGNC: 20415

OMIM: 607940

KEGG: hsa:25870

STRING: 9606.ENSP00000341938

UniGene: Hs.279696

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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