TNNI3 Antibody

Datasheet
Code CSB-PA07939A0Rb
Size US$299
Uniprot No. P19429
Image
  • Immunofluorescence staining of Hela cells with CSB-PA07939A0Rb at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunohistochemistry of paraffin-embedded human heart tissue using CSB-PA07939A0Rb at dilution of 1:100

  • Immunoprecipitating TNNI3 in Rat heart tissue
    Lane 1: Rabbit control IgG instead of CSB-PA07939A0Rb in Rat heart tissue. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/5000)
    Lane 2: CSB-PA07939A0Rb (8µg) + Rat heart tissue (500µg)
    Lane 3: Rat heart tissue (20µg)

Immunogen Recombinant Human Troponin I, cardiac muscle protein (2-210AA)
Raised in Rabbit
Species Reactivity Human, Rat
Tested Applications ELISA, IHC, IF, IP; Recommended dilution: IHC:1:20-1:200, IF:1:200-1:500, IP:1:200-1:2000
Relevance Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Troponin I, cardiac muscle (Cardiac troponin I), TNNI3, TNNC1
Immunogen Species Human
Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol
Research Area Signal Transduction
Target Names TNNI3
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.
Involvement in disease Cardiomyopathy, familial hypertrophic 7 (CMH7); Cardiomyopathy, familial restrictive 1 (RCM1); Cardiomyopathy, dilated 2A (CMD2A); Cardiomyopathy, dilated 1FF (CMD1FF)
Protein Families Troponin I family
Database Links

HGNC: 11947

OMIM: 115210

KEGG: hsa:7137

STRING: 9606.ENSP00000341838

UniGene: Hs.709179

Pathway cAMP signaling pathway
Cardiac muscle contraction

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