UBE2D2 Antibody

Datasheet

Code	CSB-PA025444LA01HU
Size	US$166
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Image	Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA025444LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human endometrial cancer using CSB-PA025444LA01HU at dilution of 1:100 Immunofluorescence staining of Hela cells with CSB-PA025444LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) UBE2D2 Polyclonal antibody

Uniprot No.

P62837

Target Names

UBE2D2

Alternative Names

E2(17)KB2 antibody; OTTHUMP00000223475 antibody; OTTHUMP00000223476 antibody; OTTHUMP00000223477 antibody; OTTHUMP00000223478 antibody; OTTHUMP00000224375 antibody; PUBC 1 antibody; PUBC1 antibody; UB2D2_HUMAN antibody; UBC 4 antibody; UBC 4/5 antibody; UBC4 antibody; UBC4/5 antibody; UBC4/5 homolog yeast antibody; UBCH 5B antibody; UBCH5B antibody; UBE2D2 antibody; Ubiquitin carrier protein antibody; Ubiquitin carrier protein D2 antibody; Ubiquitin conjugating enzyme E2 17 kDa 2 antibody; Ubiquitin conjugating enzyme E2 D2 antibody; Ubiquitin conjugating enzyme E2D 2 (homologous to yeast UBC4/5) antibody; Ubiquitin conjugating enzyme E2D 2 (UBC4/5 homolog yeast) antibody; Ubiquitin conjugating enzyme E2D 2 antibody; Ubiquitin protein ligase D2 antibody; Ubiquitin-conjugating enzyme E2 D2 antibody; Ubiquitin-conjugating enzyme E2(17)KB 2 antibody; Ubiquitin-conjugating enzyme E2-17 kDa 2 antibody; Ubiquitin-protein ligase D2 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Ubiquitin-conjugating enzyme E2 D2 protein (1-147AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The UBE2D2 Antibody (Product code: CSB-PA025444LA01HU) is Non-conjugated. For UBE2D2 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA025444LB01HU	UBE2D2 Antibody, HRP conjugated	ELISA
FITC	CSB-PA025444LC01HU	UBE2D2 Antibody, FITC conjugated
Biotin	CSB-PA025444LD01HU	UBE2D2 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and autoubiquitination of STUB1 and TRAF6. Involved in the signal-induced conjugation and subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination and degradation, MDM2-dependent degradation of p53/TP53 and the activation of MAVS in the mitochondria by DDX58/RIG-I in response to viral infection. Essential for viral activation of IRF3.

Gene References into Functions

Knockdown of UBE2D2 caused an increase in p53 protein levels, and knockdown of p53 attenuated not only cadmium-induced apoptosis, but also cadmium-induced apoptosis-related gene expression. PMID: 26912277
mutations having marked effects on function only minimally affect the intermolecular interactions between the AO7 RING and UbcH5B, establishing a high degree of complexity in activation through the RING-E2 interface. PMID: 26475854
together with UBE2L3 and UBE2N, synergistically contribute to Parkin-mediated mitophagy PMID: 24906799
Presented is the structure of the human dimeric RING domain from BIRC7 in complex with the E2 UbcH5B covalently linked to Ub. PMID: 22902369
determined the 2.2 A crystal structure of an intermediate of UbcH5b approximately ubiquitin (Ub) conjugate, which is assembled into an infinite spiral through the backside interaction. PMID: 20152160
role of WW3 and WW4 domains of Nedd4-2 in dopamine transporter ubiquitination was demonstrated; siRNA analysis demonstrated that this polyubiquitination is mediated by Nedd4-2 cooperation with UBE2D and UBE2L3 E2 ubiquitin-conjugating enzymes PMID: 20051513
Results describe the crystal structure of a complex between the HECT domain of NEDD4L and the E2 UbcH5B bearing a covalently linked Ub at its active site (UbcH5B approximately Ub). PMID: 20064473
Data show that binding of the CNOT4 RING finger to the ubiquitin-conjugating enzyme (E2) UbcH5B is highly selective. PMID: 15001359
UbcH5B/C are E2s for Mdm2, which contribute to the maintenance of low levels of p53 and Mdm2 in unstressed cells; inhibition of p53 ubiquitination and degradation by targeting UbcH5B/C is not sufficient to up-regulate p53 transcriptional activity. PMID: 15280377
Ubc4/5 and c-Cbl continue to ubiquitinate EGF receptor after internalization to facilitate polyubiquitination and degradation PMID: 18508924
UBE2D2 is required for GCMa ubiquitination and for association with the SCF(FBXW2) complex. PMID: 18703417
The results imply that the interaction specificity between c-Cbl, UbcH7 and UbcH5b is required but not sufficient for transfer of ubiquitin to potential targets. PMID: 18996392

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Protein Families

Ubiquitin-conjugating enzyme family

Database Links

HGNC: 12475

OMIM: 602962

KEGG: hsa:7322

STRING: 9606.ENSP00000381717

UniGene: Hs.108332

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Protein processing in endoplasmic reticulum

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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