UBE2D2 Antibody

Code CSB-PA025444LA01HU
Size US$166
Order now
Image
  • Immunohistochemistry of paraffin-embedded human melanoma using CSB-PA025444LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human endometrial cancer using CSB-PA025444LA01HU at dilution of 1:100

  • Immunofluorescence staining of Hela cells with CSB-PA025444LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

The Latest Promotion Free Antibody trial simple
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) UBE2D2 Polyclonal antibody
Uniprot No.
Target Names
UBE2D2
Alternative Names
E2(17)KB2 antibody; OTTHUMP00000223475 antibody; OTTHUMP00000223476 antibody; OTTHUMP00000223477 antibody; OTTHUMP00000223478 antibody; OTTHUMP00000224375 antibody; PUBC 1 antibody; PUBC1 antibody; UB2D2_HUMAN antibody; UBC 4 antibody; UBC 4/5 antibody; UBC4 antibody; UBC4/5 antibody; UBC4/5 homolog yeast antibody; UBCH 5B antibody; UBCH5B antibody; UBE2D2 antibody; Ubiquitin carrier protein antibody; Ubiquitin carrier protein D2 antibody; Ubiquitin conjugating enzyme E2 17 kDa 2 antibody; Ubiquitin conjugating enzyme E2 D2 antibody; Ubiquitin conjugating enzyme E2D 2 (homologous to yeast UBC4/5) antibody; Ubiquitin conjugating enzyme E2D 2 (UBC4/5 homolog yeast) antibody; Ubiquitin conjugating enzyme E2D 2 antibody; Ubiquitin protein ligase D2 antibody; Ubiquitin-conjugating enzyme E2 D2 antibody; Ubiquitin-conjugating enzyme E2(17)KB 2 antibody; Ubiquitin-conjugating enzyme E2-17 kDa 2 antibody; Ubiquitin-protein ligase D2 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Ubiquitin-conjugating enzyme E2 D2 protein (1-147AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The UBE2D2 Antibody (Product code: CSB-PA025444LA01HU) is Non-conjugated. For UBE2D2 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA025444LB01HU UBE2D2 Antibody, HRP conjugated ELISA
FITC CSB-PA025444LC01HU UBE2D2 Antibody, FITC conjugated
Biotin CSB-PA025444LD01HU UBE2D2 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, IHC, IF
Recommended Dilution
Application Recommended Dilution
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-48'-linked polyubiquitination. Mediates the selective degradation of short-lived and abnormal proteins. Functions in the E6/E6-AP-induced ubiquitination of p53/TP53. Mediates ubiquitination of PEX5 and autoubiquitination of STUB1 and TRAF6. Involved in the signal-induced conjugation and subsequent degradation of NFKBIA, FBXW2-mediated GCM1 ubiquitination and degradation, MDM2-dependent degradation of p53/TP53 and the activation of MAVS in the mitochondria by DDX58/RIG-I in response to viral infection. Essential for viral activation of IRF3.
Gene References into Functions
  1. Knockdown of UBE2D2 caused an increase in p53 protein levels, and knockdown of p53 attenuated not only cadmium-induced apoptosis, but also cadmium-induced apoptosis-related gene expression. PMID: 26912277
  2. mutations having marked effects on function only minimally affect the intermolecular interactions between the AO7 RING and UbcH5B, establishing a high degree of complexity in activation through the RING-E2 interface. PMID: 26475854
  3. together with UBE2L3 and UBE2N, synergistically contribute to Parkin-mediated mitophagy PMID: 24906799
  4. Presented is the structure of the human dimeric RING domain from BIRC7 in complex with the E2 UbcH5B covalently linked to Ub. PMID: 22902369
  5. determined the 2.2 A crystal structure of an intermediate of UbcH5b approximately ubiquitin (Ub) conjugate, which is assembled into an infinite spiral through the backside interaction. PMID: 20152160
  6. role of WW3 and WW4 domains of Nedd4-2 in dopamine transporter ubiquitination was demonstrated; siRNA analysis demonstrated that this polyubiquitination is mediated by Nedd4-2 cooperation with UBE2D and UBE2L3 E2 ubiquitin-conjugating enzymes PMID: 20051513
  7. Results describe the crystal structure of a complex between the HECT domain of NEDD4L and the E2 UbcH5B bearing a covalently linked Ub at its active site (UbcH5B approximately Ub). PMID: 20064473
  8. Data show that binding of the CNOT4 RING finger to the ubiquitin-conjugating enzyme (E2) UbcH5B is highly selective. PMID: 15001359
  9. UbcH5B/C are E2s for Mdm2, which contribute to the maintenance of low levels of p53 and Mdm2 in unstressed cells; inhibition of p53 ubiquitination and degradation by targeting UbcH5B/C is not sufficient to up-regulate p53 transcriptional activity. PMID: 15280377
  10. Ubc4/5 and c-Cbl continue to ubiquitinate EGF receptor after internalization to facilitate polyubiquitination and degradation PMID: 18508924
  11. UBE2D2 is required for GCMa ubiquitination and for association with the SCF(FBXW2) complex. PMID: 18703417
  12. The results imply that the interaction specificity between c-Cbl, UbcH7 and UbcH5b is required but not sufficient for transfer of ubiquitin to potential targets. PMID: 18996392

Show More

Hide All

Protein Families
Ubiquitin-conjugating enzyme family
Database Links

HGNC: 12475

OMIM: 602962

KEGG: hsa:7322

STRING: 9606.ENSP00000381717

UniGene: Hs.108332

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1
webinars: DT3C facilitates antibody internalization X
Place an order now

I. Product details

*
*
*
*

II. Contact details

*
*

III. Ship To

*
*
*
*
*
*
*

IV. Bill To

*
*
*
*
*
*
*
*