VCP Antibody, HRP conjugated

Code CSB-PA025813LB01HU
Size US$299
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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) VCP Polyclonal antibody
Uniprot No. P55072
Target Names VCP
Alternative Names 15S Mg(2+) ATPase p97 subunit antibody; 15S Mg(2+)-ATPase p97 subunit antibody; ALS14 antibody; ATPase p97 antibody; CDC48 antibody; IBMPFD antibody; MGC131997 antibody; MGC148092 antibody; MGC8560 antibody; p97 antibody; TER ATPase antibody; TERA antibody; TERA_HUMAN antibody; Transitional endoplasmic reticulum ATPase antibody; Valosin containing protein antibody; Valosin-containing protein antibody; VCP antibody; Yeast Cdc48p homolog antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Transitional endoplasmic reticulum ATPase protein (704-806AA)
Immunogen Species Homo sapiens (Human)
Conjugate HRP
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA
Protocols ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
(From Uniprot)
Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. Regulates E3 ubiquitin-protein ligase activity of RNF19A. Component of the VCP/p97-AMFR/gp78 complex that participates in the final step of the sterol-mediated ubiquitination and endoplasmic reticulum-associated degradation (ERAD) of HMGCR. Involved in endoplasmic reticulum stress-induced pre-emptive quality control, a mechanism that selectively attenuates the translocation of newly synthesized proteins into the endoplasmic reticulum and reroutes them to the cytosol for proteasomal degradation
Gene References into Functions
  1. These results indicate that the outer membrane protein MCL1 is degraded by the VCP-UBXD1 complex and that the process is promoted by the presence of mutant Huntingtin. PMID: 27913212
  2. Study results suggest that VCP recruitment to mitochondria by mtHtt is a crucial step in the initiation of neuropathology in Huntington's disease. PMID: 27561680
  3. ASPL efficiently promotes p97 hexamer disassembly, resulting in the formation of stable p97:ASPL. Overproduction of ASPL disrupts p97 hexamer function in endoplasmic reticulum-associated protein degradation. PMID: 27762274
  4. Data indicate that approximately 9% of patients with valosin-containing protein (VCP) mutations had an amyotrophic lateral sclerosis (ALS) phenotype, 4% had been diagnosed with Parkinson's disease (PD), and 2% had been diagnosed with Alzheimer's disease (AD). PMID: 28692196
  5. CB-5083 decreases viability in multiple myeloma cell lines and patient-derived multiple myeloma cells, including those with background proteasome inhibitor (PI) resistance. CB-5083 has a unique mechanism of action that combines well with PIs, which is likely owing to the p97-dependent retro-translocation of the transcription factor, Nrf1, which transcribes proteasome subunit genes following exposure to a PI PMID: 28878026
  6. a p97 mutant that causes inclusion body myopathy, Paget's disease of bone, and frontotemporal dementia unfolds substrate faster, suggesting that excess activity may underlie pathogenesis PMID: 28512218
  7. Results report that VCP/p97 promotes the degradation of ubiquitylated GS, resulting in its accumulation in cells with compromised p97 function. Notably, p97 is also required for the degradation of all four known CRBN neo-substrates [IKZF1, IKZF3, CK1alpha, and GSPT1] whose ubiquitylation is induced by immunomodulatory drugs. PMID: 28320958
  8. non-cell-autonomous effects of VCP-mutant astrocytes on both control and mutant Motor neurons, were examined. PMID: 28564594
  9. AAA-ATPase p97 suppresses apoptotic and autophagy-associated cell death in rheumatoid arthritis synovial fibroblasts. PMID: 27623077
  10. Data suggest ATXN3 binds with low-micromolar affinity to both wild-type p97/VCP and mutants linked to proteostasis deficiency multisystem proteinopathy 1 (MSP1; also called hereditary inclusion body myopathy); stoichiometry of binding is one ATXN3 molecule per p97/VCP hexamer in presence of ATP; MSP1 mutants of p97/VCP bind ATXN3 irrespective of nucleotide state. (VCP = valosin-containing protein/ATPase; ATXN3 = ataxin-3) PMID: 28939772
  11. a substantial proportion of the beta2AR produced is non-functional and VCP plays a key role in the maturation and trafficking of the beta2AR. PMID: 27887991
  12. Its mutation is not found in a cohort of German patients with sporadic amyotrophic lateral sclerosis and frontotemporal lobar degeneration comorbidity. PMID: 28551275
  13. When HEK293T cells were co-transfected with IQGAP1 and VCP, an immunoprecipitation assay revealed that binding of IQGAP1 with disease-related mutant (R155H or A232E) VCP was markedly reduced compared to wild-type VCP. This suggests that reduction of IQGAP1 and VCP interaction may be associated with the pathophysiology of inclusion body myopathy with Paget's disease of bone and frontotemporal dementia (IBMPFD). PMID: 28970065
  14. Rare variants in VCP genes are risk for sporadic inclusion body myositis. PMID: 27594680
  15. Knockdown of the host ubiquitin-dependent segregase VCP/p97, results in loss of IE2 expression, subsequent suppression of early and late gene expression and, ultimately, failure in virus replication. NMS-873, a small molecule inhibitor of VCP, is a potent HCMV antiviral with potential as a novel host targeting therapeutic for HCMV infection. PMID: 28494016
  16. VCP removes sterically trapped Ku70/80 rings from DNA in double-strand break repair. PMID: 27716483
  17. Data suggest that AAA+ (ATPases associated with diverse cellular activities) ATPase p97 is essential to wide range of cellular functions, including endoplasmic reticulum-associated degradation, membrane fusion, NFkappaB (nuclear factor kappa-light-chain-enhancer of activated B cells) activation, and chromatin-associated processes, which are regulated by ubiquitination. [REVIEW] PMID: 28819009
  18. Upon damage, p97 translocates to lysosomes and there cooperates with a distinct set of cofactors including UBXD1, PLAA, and the deubiquitinating enzyme YOD1, which we term ELDR components for Endo-Lysosomal Damage Response. PMID: 27753622
  19. Data show that inhibition of VCP/p97, or siRNA-mediated ablation of VCP/p97 impairs ultraviolet radiation (UVR)-induced RNA polymerase II (RNAPII) degradation. PMID: 28036256
  20. p97 negatively regulates NRF2 through the canonical pathway by extracting ubiquitylated NRF2 from the KEAP1-CUL3 E3 complex. PMID: 28115426
  21. Data suggest that dimerization of UBX domain protein 7 (UBXD7) could affect the formation of the p97 ATPase-UBXD7 complex. PMID: 28274878
  22. a VCP mutation/knockdown-induced dysregulation in the adenine nucleotide translocase, which results in a slower rate of ADP or ATP translocation across the mitochondrial membranes. PMID: 28360103
  23. The functional motions of p97 using symmetric normal modes have been predicted. PMID: 27653958
  24. insights into the interactions between other SHP-containing proteins and p97N PMID: 27714797
  25. The findings of the present study indicated that VCP is very important for the proliferation and metastasis of colorectal cancer; therefore, targeting VCP and its downstream targets may represent novel therapies for the treatment of colorectal cancer. PMID: 27344168
  26. Timely and efficient degradation of ubiquitinated IkappaB[alpha], concomitant with timely and efficient liberation of RelA from ubiquitinated IkappaB[alpha] and RelA nuclear translocation, essentially depends on the presence of functional p97/VCP. PMID: 26463447
  27. Its gene mutation is correlated with occurrence of amyotrophic lateral sclerosis. PMID: 26511028
  28. results have revealed SUMOylation as a molecular signaling switch to regulate the distribution and functions of VCP during stress response, and suggest that deficiency in VCP SUMOylation caused by pathogenic mutations will render cells vulnerable to stress insults. PMID: 27226613
  29. depletion of VCP enzymatic activity triggers cancer cell death in part through inadequate regulation of protein synthesis and amino acid metabolism. PMID: 26720340
  30. new role of VCP/p97 segregase in the timely processing of ubiquitinated CSB from damaged chromatin. PMID: 26826127
  31. Ankrd13 proteins cooperate with VCP to regulate the lysosomal trafficking of ubiquitinated Cav-1. PMID: 26797118
  32. results suggest that human Cdc48 interacts functionally with the 20S proteasome. PMID: 26134898
  33. we show that loss of VCP induces endoplasmic reticulum stress and epithelial-mesenchymal transition PMID: 25970786
  34. interaction between SelK and p97(VCP) is SelS-dependent, and the resulting ERAD complex (SelS-p97(VCP)-SelK) plays an important role in ERAD and ER stress PMID: 26504085
  35. this study demonstrates significant correlation between the cytoplasmic expression of VCP and adverse prognosis in breast carcinoma, suggesting that VCP may serve as a prognostic biomarker in breast carcinoma. PMID: 26168958
  36. UBXD1-N intercalates into the p97-ND1 interface, thereby modulating interdomain communication of p97 domains and its activity with relevance for disease pathogenesis PMID: 26475856
  37. Our results provide the first structural clues of how VCP mutations may influence the activity and function of the D2 ATPase ring. PMID: 26549226
  38. Data indicate that ATPase p97 is a key mediator of several protein homeostasis processes and is a strong potential cancer target. PMID: 26555175
  39. activity of the p97-associated deubiquitinylase YOD1 is also required for substrate disposal PMID: 26463207
  40. specific silencing of Derlin-2, p97 and HRD1 by shRNAs increases steady state levels of proinsulin. these ERAD constituents are critically involved in proinsulin degradation and may therefore also play a role in subsequent antigen generation. PMID: 26107514
  41. A novel UGGT1- and p97-dependent protein quality checkpoint is shown. This checkpoint is alerted to prevent secretion of a polypeptide that passes the luminal quality control scrutiny by BiP and CNX but contains an intramembrane ionizable residue. PMID: 25694454
  42. Data show that UBXN10 localizes to cilia in a AAA-ATPase VCP-dependent manner and both VCP and UBXN10 are required for ciliogenesis. PMID: 26389662
  43. Valosin-containing protein is required for coronavirus exit from endosomes. PMID: 26311884
  44. Studies indicate that cofactor binding results in defined, productive p97 (also known as Cdc48, Ter94, and VCP) assemblies with specific cellular functions. PMID: 26320413
  45. A novel mutation, p.Arg487His Mutation in the VCP gene encoding valosin-containing protein (VCP) causes sporadic amyotrophic lateral sclerosis in Japanese. PMID: 25457024
  46. Data uncover an unexpected role for p97 in activation of transcription factor Nrf1 by relocalizing it from the endoplasmic reticulum lumen to the cytosol. PMID: 24448410
  47. Results support the idea that VCP is associated with the pathomechanism of sporadic amyotrophic lateral sclerosis and familial amyotrophic lateral sclerosis with a VCP mutation, presumably acting through a dominant-negative mechanism PMID: 25492614
  48. Data showed that VCP mutations are not a major cause of FALS in the UK population although novel rare variations in the 5' UTR of the VCP gene may be pathogenic. PMID: 25618255
  49. The level of VCP-intensity determined by immunohistochemistry could be an additional prognostic marker in HPV-negative (OSCC). PMID: 25463965
  50. targeting miR-129-5p/VCP signaling pathway may serve as a therapeutic strategy for osteosarcoma management PMID: 25566966

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Involvement in disease Inclusion body myopathy with early-onset Paget disease with or without frontotemporal dementia 1 (IBMPFD1); Amyotrophic lateral sclerosis 14, with or without frontotemporal dementia (ALS14); Charcot-Marie-Tooth disease 2Y (CMT2Y)
Subcellular Location Cytoplasm, cytosol, Endoplasmic reticulum, Nucleus
Protein Families AAA ATPase family
Database Links

HGNC: 12666

OMIM: 167320

KEGG: hsa:7415

STRING: 9606.ENSP00000351777

UniGene: Hs.529782

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