Code | CSB-RA182227A0HU |
Size | US$210 |
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Application | Recommended Dilution |
---|---|
WB | 1:500-1:5000 |
IHC | 1:50-1:200 |
IF | 1:20-1:200 |
IP | 1:200-1:1000 |
The VCP recombinant monoclonal antibody is used to detect human and rat VCP proteins in five applications including ELISA, WB, IHC, IF, and IP. It is produced through recombinant DNA technology by synthesizing the gene coding for the VCP monoclonal antibody after sequencing the cDNA of the VCP antibody-producing hybridomas. The hybridomas are created by fusing myeloma cells with B cells that were isolated from an animal immunized with a synthesized peptide derived from human VCP. The synthesized gene is then cloned into a vector and then transfected into cells for cultivation. The resulting VCP recombinant monoclonal antibody undergoes affinity chromatography purification.
The VCP protein, also known as p97, is a highly conserved member of the AAA+ (ATPases associated with various cellular activities) family. VCP plays a critical role in protein quality control by acting as an ATP-dependent molecular chaperone that mediates the degradation of misfolded proteins through the ubiquitin-proteasome system. It is also involved in DNA damage repair, membrane fusion, autophagy, and regulation of transcription factors. Mutations in the VCP gene have been associated with a range of human diseases, including the inclusion of body myopathy with Paget's disease of bone and frontotemporal dementia (IBMPFD).
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