WNT8A Antibody

Datasheet

Code	CSB-PA863951LA01HU
Size	US$166
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Image	IHC image of CSB-PA863951LA01HU diluted at 1:200 and staining in paraffin-embedded human gastric cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA863951LA01HU diluted at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) WNT8A Polyclonal antibody

Uniprot No.

Q9H1J5

Target Names

WNT8A

Alternative Names

Protein Wnt-8a antibody; Protein Wnt-8d antibody; wingless type MMTV integration site family, member 8A antibody; WNT 8A antibody; Wnt 8d antibody; wnt8a antibody; WNT8A_HUMAN antibody; WNT8D antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Protein Wnt-8a protein (223-309AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The WNT8A Antibody (Product code: CSB-PA863951LA01HU) is Non-conjugated. For WNT8A Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA863951LB01HU	WNT8A Antibody, HRP conjugated	ELISA
FITC	CSB-PA863951LC01HU	WNT8A Antibody, FITC conjugated
Biotin	CSB-PA863951LD01HU	WNT8A Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Ligand for members of the frizzled family of seven transmembrane receptors. Plays a role in embryonic patterning.

Gene References into Functions

Here, we show that Wnt/planar cell polarity (PCP) autocrine signaling controls the emergence of cytonemes, and that cytonemes subsequently control paracrine Wnt/beta-catenin signal activation. Upon binding of the Wnt family member Wnt8a, the receptor tyrosine kinase Ror2 becomes activated. PMID: 30060804
the WNT8A gene is involved in the susceptibility to HSCR, and plays an important role in the occurrence and development of Hirschsprung's disease. PMID: 23836442
Expression and regulation of WNT8A mRNA in human tumor cell lines PMID: 11956596

Subcellular Location

Secreted, extracellular space, extracellular matrix. Secreted.

Protein Families

Wnt family

Database Links

HGNC: 12788

OMIM: 606360

KEGG: hsa:7478

STRING: 9606.ENSP00000381739

UniGene: Hs.591274

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Protein Service

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The overview of Wnt signaling pathway Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Hippo signaling pathway Melanogenesis mTOR signaling pathway Wnt signaling pathway Signaling pathways regulating pluripotency of stem cells

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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