ZFYVE9 Antibody

Datasheet

Code	CSB-PA026477LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, L02 whole cell lysate, U87 whole cell lysate, HepG2 whole cell lysate All lanes: ZFYVE9 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 157, 151, 84 kDa Observed band size: 157 kDa Immunofluorescence staining of HepG2 cells with CSB-PA026477LA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ZFYVE9 Polyclonal antibody

Uniprot No.

O95405

Target Names

ZFYVE9

Alternative Names

CG15667 antibody; hSARA antibody; LD33044p antibody; Madh-interacting protein antibody; MADHIP antibody; Mothers against decapentaplegic homolog-interacting protein antibody; Novel serine protease antibody; NSP antibody; Receptor activation anchor antibody; SAR1 antibody; SARA antibody; SARA1 antibody; Smad anchor for receptor activation antibody; SMADIP antibody; ZFYV9_HUMAN antibody; ZFYVE9 antibody; Zinc finger FYVE domain-containing protein 9 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Zinc finger FYVE domain-containing protein 9 protein (683-926AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ZFYVE9 Antibody (Product code: CSB-PA026477LA01HU) is Non-conjugated. For ZFYVE9 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA026477LB01HU	ZFYVE9 Antibody, HRP conjugated	ELISA
FITC	CSB-PA026477LC01HU	ZFYVE9 Antibody, FITC conjugated
Biotin	CSB-PA026477LD01HU	ZFYVE9 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Early endosomal protein that functions to recruit SMAD2/SMAD3 to intracellular membranes and to the TGF-beta receptor. Plays a significant role in TGF-mediated signaling by regulating the subcellular location of SMAD2 and SMAD3 and modulating the transcriptional activity of the SMAD3/SMAD4 complex. Possibly associated with TGF-beta receptor internalization.

Gene References into Functions

we have identified endofin as an important signalling component required for basal and BMP-induced hepcidin expression. PMID: 26358513
SARA may serve as a potential novel target in pre-Epithelial-mesenchymal transition states for the amelioration renal fibrosis seen in chronic kidney diseases PMID: 26159183
PI3K-C2a was also required for TGFb receptor-mediated formation of SARA-Smad2/3 complex PMID: 25614622
The negative influence that perturbation of RNF11 and SARA levels exerts on the lysosomal degradation of EGFRs could underscore the significance of overexpression of RNF11 in certain cancers. PMID: 23222715
TGF-beta1 can induce epithelial-to-mesenchymal transition through reduction in SARA expression, SARA is also basally regulated by its interaction with PI3K. PMID: 22942286
no correlation between SARA expression and the levels of TGF-beta1-induced phosphorylation of Smads in various B-cell lymphomas PMID: 22819827
SARA binds to ERBIN using a new domain, which we have called the ERBID (ERBIN-binding domain) PMID: 21878490
After stimulation with glucose, expression of SARA decreased in a time-dependent manner in epithelium to mesenchymal transition of proximal tubule cells. PMID: 21200089
Expression of a SARA mutant protein lacking the FYVE finger inhibits downstream activin A signaling in endothelial cells. PMID: 11877415
role in rab5 mediated endocytosis PMID: 12432064
SARA MH2 domains function in TGF-beta signaling PMID: 15231848
internalization is important for transforming growth factor beta1-induced Smad2 association with Smad anchor for receptor activation (SARA) and Smad2-dependent signaling in human mesangial cells PMID: 15613484
SARA acts as a Smad anchor for receptor activation in BMP signaling. PMID: 17356069
Protein-protein and protein-lipid interactions organized by SARA regulate the vesicular targeting of rhodopsin-bearing axonemal vesicles to nascent discs at the base of the rod outer segment. PMID: 17693260
SARA has a role in regulating cell phenotype and its effects are mediated through modification of the balance between Smad2 and Smad3 signaling PMID: 19620243

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Subcellular Location

Cytoplasm. Early endosome membrane.

Tissue Specificity

Ubiquitous. In the brain found primarily in the cerebrovascular smooth muscle cells and reactive astrocytes.

Database Links

HGNC: 6775

OMIM: 603755

KEGG: hsa:9372

STRING: 9606.ENSP00000287727

UniGene: Hs.532345

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

TGF-beta signaling pathway Endocytosis

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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