ZNF268 Antibody

Datasheet
Code CSB-PA026625LA01HU
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Size US$299
Uniprot No. Q14587
Image
  • Western Blot
    Positive WB detected in: 293T whole cell lysate, K562 whole cell lysate
    All lanes: ZNF268 antibody at 4µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 109, 90, 10, 12, 16, 6, 13 kDa
    Observed band size: 109 kDa

  • Immunofluorescence staining of Hela cells with CSB-PA026625LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Immunogen Recombinant Human Zinc finger protein 268 protein (416-603AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IF; Recommended dilution: WB:1:500-1:5000, IF:1:50-1:200
Relevance Isoform 1: Acts as a transcriptional repressor. Inhibits erythroid differentiation and tumor cell proliferation. Plays a role during ovarian cancer development and progression.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Zinc finger protein 268 (Zinc finger protein HZF3), ZNF268
Immunogen Species Human
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Research Area Others
Target Names ZNF268
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Isoform 1
Subcellular Location Isoform 1: Nucleus, SUBCELLULAR LOCATION: Isoform 2: Nucleus, Cytoplasm
Protein Families Krueppel C2H2-type zinc-finger protein family
Tissue Specificity Overexpressed in ovarian cancer tissues compared to normal ovarian tissues. Isoform 1 and isoform 2 are expressed in squamous epithelium tissues. Isoform 2 is overexpressed in squamous cervical cancer (at protein level). Expressed in blood cells. Isoform
Database Links

HGNC: 13061

OMIM: 604753

KEGG: hsa:10795

STRING: 9606.ENSP00000228289

UniGene: Hs.124047

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