oprF Antibody

Code CSB-PA318417LA01EZX
Size US$166
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  • Western Blot
    Positive WB detected in Recombinant protein
    All lanes: oprF antibody at 5μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 39.3 kDa
    Observed band size: 44 kDa
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Product Details

Full Product Name
Rabbit anti-Pseudomonas aeruginosa oprF Polyclonal antibody
Uniprot No.
Target Names
oprF
Alternative Names
oprF antibody; PA1777 antibody; Outer membrane porin F antibody
Raised in
Rabbit
Species Reactivity
Pseudomonas aeruginosa
Immunogen
Recombinant Pseudomonas aeruginosa Outer membrane porin F protein (25-350AA)
Immunogen Species
Pseudomonas aeruginosa
Conjugate
Non-conjugated

The oprF Antibody (Product code: CSB-PA318417LA01EZX) is Non-conjugated. For oprF Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA318417LB01EZX oprF Antibody, HRP conjugated ELISA
FITC CSB-PA318417LC01EZX oprF Antibody, FITC conjugated
Biotin CSB-PA318417LD01EZX oprF Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The OprF polyclonal antibody is a rabbit antibody that specifically recognizes Pseudomonas aeruginosa OprF protein. Its immunogen is the recombinant Pseudomonas aeruginosa OprF protein (25-350aa). It has been validated for use in ELISA and WB assays. The antibody has been purified using protein G and has a purity level of over 95%.

OprF is one of the major porins in the outer membrane of P. aeruginosa, participating in the uptake of nutrients and antibiotics, as well as in the efflux of toxins and metabolic waste products. It has also been implicated in virulence and pathogenesis in P. aeruginosa.

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Target Background

Function
Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium.
Gene References into Functions
  1. OprF (an outer membrane porin, highly conserved in the Pseudomonas) binds human C3b. PMID: 25964476
  2. LecB binds to the outer membrane protein OprF PMID: 23056489
  3. oprF transcription was increased in response to low NaCl or high sucrose concentrations, and this induced transcription was strongly impaired in the absence of SigX. PMID: 22685281
  4. Prevention of disulfide bond formation in OprF increases its pore-forming activity. PMID: 20978537
  5. This is the first study showing a link between OprF, PQS synthesis, T3SS, and virulence factor production PMID: 21189321
  6. Data show that the recombinant plasmid pIRES-tPA-OprF-MyD88 has been successfully constructed and tPA-OprF and MyD88 protein can be highly expressed in transfected cells. PMID: 19257978
  7. findings suggest that OprF could adopt two alternative conformations in the outer membrane and that folding is thermoregulated PMID: 15528532
  8. findings demonstrate that interferon-gamma binds to an outer membrane protein in Pseudomonas aeruginosa, OprF, resulting in the expression of a quorum-sensing dependent virulence determinant, the PA-I lectin PMID: 16051797
  9. analysis of OprF from Pseudomonas aeruginosa PMID: 16397890
  10. OprF exists in two different conformations PMID: 16595653
  11. analysis of Pseudomonas aeruginosa porin OprF PMID: 16617058
  12. Major outer membrane protein F (OprF) is identified as a protein with important functions; mutant Pseudomonas aeruginosa deficient in OprF negates the antibacterial role of host neutrophil elastase both in vitro and in a pneumonia model. PMID: 18802098

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Subcellular Location
Cell outer membrane; Multi-pass membrane protein.
Protein Families
OmpA family
Database Links

KEGG: pae:PA1777

STRING: 208964.PA1777

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