oprF Antibody

Datasheet

Code	CSB-PA318417LA01EZX
Size	US$166
Image	Western Blot Positive WB detected in Recombinant protein All lanes: oprF antibody at 5μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 39.3 kDa Observed band size: 44 kDa
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Target Background

Product Details

Full Product Name

Rabbit anti-Pseudomonas aeruginosa oprF Polyclonal antibody

Uniprot No.

P13794

Target Names

oprF

Alternative Names

oprF antibody; PA1777 antibody; Outer membrane porin F antibody

Raised in

Rabbit

Species Reactivity

Pseudomonas aeruginosa

Immunogen

Recombinant Pseudomonas aeruginosa Outer membrane porin F protein (25-350AA)

Immunogen Species

Pseudomonas aeruginosa

Conjugate

Non-conjugated

The oprF Antibody (Product code: CSB-PA318417LA01EZX) is Non-conjugated. For oprF Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA318417LB01EZX	oprF Antibody, HRP conjugated	ELISA
FITC	CSB-PA318417LC01EZX	oprF Antibody, FITC conjugated
Biotin	CSB-PA318417LD01EZX	oprF Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The OprF polyclonal antibody is a rabbit antibody that specifically recognizes Pseudomonas aeruginosa OprF protein. Its immunogen is the recombinant Pseudomonas aeruginosa OprF protein (25-350aa). It has been validated for use in ELISA and WB assays. The antibody has been purified using protein G and has a purity level of over 95%.

OprF is one of the major porins in the outer membrane of P. aeruginosa, participating in the uptake of nutrients and antibiotics, as well as in the efflux of toxins and metabolic waste products. It has also been implicated in virulence and pathogenesis in P. aeruginosa.

Citations

Biophysical and proteomic analyses of Pseudomonas syringae pv. tomato DC3000 extracellular vesicles suggest adaptive functions during plant infection M Janda,mBio,2023

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Target Background

Function

Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium.

Gene References into Functions

OprF (an outer membrane porin, highly conserved in the Pseudomonas) binds human C3b. PMID: 25964476
LecB binds to the outer membrane protein OprF PMID: 23056489
oprF transcription was increased in response to low NaCl or high sucrose concentrations, and this induced transcription was strongly impaired in the absence of SigX. PMID: 22685281
Prevention of disulfide bond formation in OprF increases its pore-forming activity. PMID: 20978537
This is the first study showing a link between OprF, PQS synthesis, T3SS, and virulence factor production PMID: 21189321
Data show that the recombinant plasmid pIRES-tPA-OprF-MyD88 has been successfully constructed and tPA-OprF and MyD88 protein can be highly expressed in transfected cells. PMID: 19257978
findings suggest that OprF could adopt two alternative conformations in the outer membrane and that folding is thermoregulated PMID: 15528532
findings demonstrate that interferon-gamma binds to an outer membrane protein in Pseudomonas aeruginosa, OprF, resulting in the expression of a quorum-sensing dependent virulence determinant, the PA-I lectin PMID: 16051797
analysis of OprF from Pseudomonas aeruginosa PMID: 16397890
OprF exists in two different conformations PMID: 16595653
analysis of Pseudomonas aeruginosa porin OprF PMID: 16617058
Major outer membrane protein F (OprF) is identified as a protein with important functions; mutant Pseudomonas aeruginosa deficient in OprF negates the antibacterial role of host neutrophil elastase both in vitro and in a pneumonia model. PMID: 18802098

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Subcellular Location

Cell outer membrane; Multi-pass membrane protein.

Protein Families

OmpA family

Database Links

KEGG: pae:PA1777

STRING: 208964.PA1777

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Service

Phage Display Service

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Protein Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 322 (CUBIO Innovation Center), Houston, TX 77054, USA

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oprF Antibody

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background