Recombinant Pseudomonas aeruginosa Outer membrane porin F (oprF)

Code CSB-YP318417EZX
MSDS
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Source Yeast
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Code CSB-BP318417EZX
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP318417EZX
MSDS
Size Pls inquire
Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
oprF
Uniprot No.
Alternative Names
oprF; PA1777; Outer membrane porin F
Species
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Expression Region
25-350
Target Protein Sequence
QGQNSV EIEAFGKRYF TDSVRNMKNA DLYGGSIGYF LTDDVELALS YGEYHDVRGT YETGNKKVHG NLTSLDAIYH FGTPGVGLRP YVSAGLAHQN ITNINSDSQG RQQMTMANIG AGLKYYFTEN FFAKASLDGQ YGLEKRDNGH QGEWMAGLGV GFNFGGSKAA PAPEPVADVC SDSDNDGVCD NVDKCPDTPA NVTVDANGCP AVAEVVRVQL DVKFDFDKSK VKENSYADIK NLADFMKQYP STSTTVEGHT DSVGTDAYNQ KLSERRANAV RDVLVNEYGV EGGRVNAVGY GESRPVADNA TAEGRAINRR VEAEVEAEAK
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium.
Gene References into Functions
  1. OprF (an outer membrane porin, highly conserved in the Pseudomonas) binds human C3b. PMID: 25964476
  2. LecB binds to the outer membrane protein OprF PMID: 23056489
  3. oprF transcription was increased in response to low NaCl or high sucrose concentrations, and this induced transcription was strongly impaired in the absence of SigX. PMID: 22685281
  4. Prevention of disulfide bond formation in OprF increases its pore-forming activity. PMID: 20978537
  5. This is the first study showing a link between OprF, PQS synthesis, T3SS, and virulence factor production PMID: 21189321
  6. Data show that the recombinant plasmid pIRES-tPA-OprF-MyD88 has been successfully constructed and tPA-OprF and MyD88 protein can be highly expressed in transfected cells. PMID: 19257978
  7. findings suggest that OprF could adopt two alternative conformations in the outer membrane and that folding is thermoregulated PMID: 15528532
  8. findings demonstrate that interferon-gamma binds to an outer membrane protein in Pseudomonas aeruginosa, OprF, resulting in the expression of a quorum-sensing dependent virulence determinant, the PA-I lectin PMID: 16051797
  9. analysis of OprF from Pseudomonas aeruginosa PMID: 16397890
  10. OprF exists in two different conformations PMID: 16595653
  11. analysis of Pseudomonas aeruginosa porin OprF PMID: 16617058
  12. Major outer membrane protein F (OprF) is identified as a protein with important functions; mutant Pseudomonas aeruginosa deficient in OprF negates the antibacterial role of host neutrophil elastase both in vitro and in a pneumonia model. PMID: 18802098

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Subcellular Location
Cell outer membrane; Multi-pass membrane protein.
Protein Families
OmpA family
Database Links

KEGG: pae:PA1777

STRING: 208964.PA1777

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