Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

oprF

Uniprot No.

P13794

Research Area

others

Alternative Names

oprF; PA1777; Outer membrane porin F

Species

Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)

Source

E.coli

Expression Region

25-350aa

Target Protein Sequence

QGQNSVEIEAFGKRYFTDSVRNMKNADLYGGSIGYFLTDDVELALSYGEYHDVRGTYETGNKKVHGNLTSLDAIYHFGTPGVGLRPYVSAGLAHQNITNINSDSQGRQQMTMANIGAGLKYYFTENFFAKASLDGQYGLEKRDNGHQGEWMAGLGVGFNFGGSKAAPAPEPVADVCSDSDNDGVCDNVDKCPDTPANVTVDANGCPAVAEVVRVQLDVKFDFDKSKVKENSYADIKNLADFMKQYPSTSTTVEGHTDSVGTDAYNQKLSERRANAVRDVLVNEYGVEGGRVNAVGYGESRPVADNATAEGRAINRRVEAEVEAEAK
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

39.3 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 25-350 constitute the expression domain of recombinant Pseudomonas aeruginosa oprF. This oprF protein is theoretically predicted to have a molecular weight of 39.3 kDa. The oprF protein was expressed in e.coli. The N-terminal 6xHis tag was smoothly integrated into the coding gene of oprF, which enables a simple process of detecting and purifying the oprF recombinant protein in the following steps.

Pseudomonas aeruginosa outer membrane porin F (OprF) is a crucial component of the outer membrane in the Gram-negative bacterium Pseudomonas aeruginosa. OprF serves as a porin, forming channels in the outer membrane that allow the passage of small molecules, including nutrients and ions. OprF plays a vital role in the permeability barrier of the bacterial cell envelope, contributing to the selective transport of substances across the outer membrane. OprF has been implicated in various physiological processes, including nutrient uptake, resistance to environmental stressors, and interactions with host cells during infection. Additionally, studies on OprF may contribute to the development of strategies to enhance antibiotic susceptibility or disrupt bacterial biofilm formation, as Pseudomonas aeruginosa is known for its ability to form biofilms, leading to chronic infections in various clinical settings. Investigating OprF and its interactions can provide valuable insights into the pathogenesis of Pseudomonas aeruginosa and aid in the development of novel antimicrobial approaches.

Target Background

Function

Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium.

Gene References into Functions

OprF (an outer membrane porin, highly conserved in the Pseudomonas) binds human C3b. PMID: 25964476
LecB binds to the outer membrane protein OprF PMID: 23056489
oprF transcription was increased in response to low NaCl or high sucrose concentrations, and this induced transcription was strongly impaired in the absence of SigX. PMID: 22685281
Prevention of disulfide bond formation in OprF increases its pore-forming activity. PMID: 20978537
This is the first study showing a link between OprF, PQS synthesis, T3SS, and virulence factor production PMID: 21189321
Data show that the recombinant plasmid pIRES-tPA-OprF-MyD88 has been successfully constructed and tPA-OprF and MyD88 protein can be highly expressed in transfected cells. PMID: 19257978
findings suggest that OprF could adopt two alternative conformations in the outer membrane and that folding is thermoregulated PMID: 15528532
findings demonstrate that interferon-gamma binds to an outer membrane protein in Pseudomonas aeruginosa, OprF, resulting in the expression of a quorum-sensing dependent virulence determinant, the PA-I lectin PMID: 16051797
analysis of OprF from Pseudomonas aeruginosa PMID: 16397890
OprF exists in two different conformations PMID: 16595653
analysis of Pseudomonas aeruginosa porin OprF PMID: 16617058
Major outer membrane protein F (OprF) is identified as a protein with important functions; mutant Pseudomonas aeruginosa deficient in OprF negates the antibacterial role of host neutrophil elastase both in vitro and in a pneumonia model. PMID: 18802098

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Subcellular Location

Cell outer membrane; Multi-pass membrane protein.

Protein Families

OmpA family

Database Links

KEGG: pae:PA1777

STRING: 208964.PA1777

Code	CSB-EP318417EZX
Abbreviation	Recombinant Pseudomonas aeruginosa oprF protein
MSDS	MSDS Datasheet
Size	US$306
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Recombinant Pseudomonas aeruginosa Outer membrane porin F (oprF)

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