Purity
Greater than 85% as determined by SDS-PAGE.
Alternative Names
oprF; PA1777; Outer membrane porin F
Species
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Expression Region
25-350aa
Target Protein Sequence
QGQNSVEIEAFGKRYFTDSVRNMKNADLYGGSIGYFLTDDVELALSYGEYHDVRGTYETGNKKVHGNLTSLDAIYHFGTPGVGLRPYVSAGLAHQNITNINSDSQGRQQMTMANIGAGLKYYFTENFFAKASLDGQYGLEKRDNGHQGEWMAGLGVGFNFGGSKAAPAPEPVADVCSDSDNDGVCDNVDKCPDTPANVTVDANGCPAVAEVVRVQLDVKFDFDKSKVKENSYADIKNLADFMKQYPSTSTTVEGHTDSVGTDAYNQKLSERRANAVRDVLVNEYGVEGGRVNAVGYGESRPVADNATAEGRAINRRVEAEVEAEAK
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Amino acids 25-350 constitute the expression domain of recombinant Pseudomonas aeruginosa oprF. This oprF protein is theoretically predicted to have a molecular weight of 39.3 kDa. The oprF protein was expressed in e.coli. The N-terminal 6xHis tag was smoothly integrated into the coding gene of oprF, which enables a simple process of detecting and purifying the oprF recombinant protein in the following steps.
Pseudomonas aeruginosa outer membrane porin F (OprF) is a crucial component of the outer membrane in the Gram-negative bacterium Pseudomonas aeruginosa. OprF serves as a porin, forming channels in the outer membrane that allow the passage of small molecules, including nutrients and ions. OprF plays a vital role in the permeability barrier of the bacterial cell envelope, contributing to the selective transport of substances across the outer membrane. OprF has been implicated in various physiological processes, including nutrient uptake, resistance to environmental stressors, and interactions with host cells during infection. Additionally, studies on OprF may contribute to the development of strategies to enhance antibiotic susceptibility or disrupt bacterial biofilm formation, as Pseudomonas aeruginosa is known for its ability to form biofilms, leading to chronic infections in various clinical settings. Investigating OprF and its interactions can provide valuable insights into the pathogenesis of Pseudomonas aeruginosa and aid in the development of novel antimicrobial approaches.
