Recombinant Pseudomonas aeruginosa Outer membrane porin F (oprF)

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Code CSB-EP318417EZX
Size US$306
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Greater than 85% as determined by SDS-PAGE.
Target Names
Uniprot No.
Research Area
Alternative Names
oprF; PA1777; Outer membrane porin F
Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1)
Expression Region
Target Protein Sequence
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
39.3 kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-tagged
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

Amino acids 25-350 constitute the expression domain of recombinant Pseudomonas aeruginosa oprF. This oprF protein is theoretically predicted to have a molecular weight of 39.3 kDa. The oprF protein was expressed in e.coli. The N-terminal 6xHis tag was smoothly integrated into the coding gene of oprF, which enables a simple process of detecting and purifying the oprF recombinant protein in the following steps.

Pseudomonas aeruginosa outer membrane porin F (OprF) is a crucial component of the outer membrane in the Gram-negative bacterium Pseudomonas aeruginosa. OprF serves as a porin, forming channels in the outer membrane that allow the passage of small molecules, including nutrients and ions. OprF plays a vital role in the permeability barrier of the bacterial cell envelope, contributing to the selective transport of substances across the outer membrane. OprF has been implicated in various physiological processes, including nutrient uptake, resistance to environmental stressors, and interactions with host cells during infection. Additionally, studies on OprF may contribute to the development of strategies to enhance antibiotic susceptibility or disrupt bacterial biofilm formation, as Pseudomonas aeruginosa is known for its ability to form biofilms, leading to chronic infections in various clinical settings. Investigating OprF and its interactions can provide valuable insights into the pathogenesis of Pseudomonas aeruginosa and aid in the development of novel antimicrobial approaches.

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Target Background

Has porin activity, forming small water-filled channels. Also has a structural role in determining cell shape and ability to grow in low-osmolarity medium.
Gene References into Functions
  1. OprF (an outer membrane porin, highly conserved in the Pseudomonas) binds human C3b. PMID: 25964476
  2. LecB binds to the outer membrane protein OprF PMID: 23056489
  3. oprF transcription was increased in response to low NaCl or high sucrose concentrations, and this induced transcription was strongly impaired in the absence of SigX. PMID: 22685281
  4. Prevention of disulfide bond formation in OprF increases its pore-forming activity. PMID: 20978537
  5. This is the first study showing a link between OprF, PQS synthesis, T3SS, and virulence factor production PMID: 21189321
  6. Data show that the recombinant plasmid pIRES-tPA-OprF-MyD88 has been successfully constructed and tPA-OprF and MyD88 protein can be highly expressed in transfected cells. PMID: 19257978
  7. findings suggest that OprF could adopt two alternative conformations in the outer membrane and that folding is thermoregulated PMID: 15528532
  8. findings demonstrate that interferon-gamma binds to an outer membrane protein in Pseudomonas aeruginosa, OprF, resulting in the expression of a quorum-sensing dependent virulence determinant, the PA-I lectin PMID: 16051797
  9. analysis of OprF from Pseudomonas aeruginosa PMID: 16397890
  10. OprF exists in two different conformations PMID: 16595653
  11. analysis of Pseudomonas aeruginosa porin OprF PMID: 16617058
  12. Major outer membrane protein F (OprF) is identified as a protein with important functions; mutant Pseudomonas aeruginosa deficient in OprF negates the antibacterial role of host neutrophil elastase both in vitro and in a pneumonia model. PMID: 18802098

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Subcellular Location
Cell outer membrane; Multi-pass membrane protein.
Protein Families
OmpA family
Database Links

KEGG: pae:PA1777

STRING: 208964.PA1777

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