Recombinant Arabidopsis thaliana High mobility group B protein 1 (HMGB1)

Code CSB-YP523735DOA
MSDS
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Source Yeast
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Code CSB-EP523735DOA
MSDS
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Source E.coli
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Code CSB-EP523735DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP523735DOA
MSDS
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Source Baculovirus
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Code CSB-MP523735DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
HMGB1; HMGA; HMGALPHA; NFD01; NFD1; ORF13; At3g51880; ATEM1.13; High mobility group B protein 1; High mobility group protein A; AtHMGalpha; HMG alpha; Nucleosome/chromatin assembly factor group D 01; Nucleosome/chromatin assembly factor group D 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-178
Target Protein Sequence
MKTAKGKDKV KTTKEALKPV DDRKVGKRKA PAEKPTKRET RKEKKAKKDP NKPKRAPSAF FVFLEDFRVT FKKENPNVKA VSAVGKAGGQ KWKSMSQAEK APYEEKAAKR KAEYEKQMDA YNKNLEEGSD ESEKSRSEIN DEDEASGEEE LLEKEAAGDD EEEEEEEDDD DDDDEEED
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Binds preferentially double-stranded DNA. Modulates general plant growth and stress tolerance. Confers sensitivity to salt and genotoxic (methyl methanesulfonate, MMS) stresses.
Gene References into Functions
  1. Results show not only HMGB1 was associated with interphase but also with metaphase chromosomes in Arabidopsis. PMID: 26270959
  2. AtHMGB1 does not affect telomerase activity and AtHMGB1 loss or overexpression does not cause any obvious changes in chromatin architecture. PMID: 21348847
  3. The subcellular localization of Arabidopsis thaliana HMGA, HMGB1, and HMGB5, reveals that they localize to the cell nucleus. They display a speckled distribution pattern throughout the chromatin of interphase nuclei. PMID: 17114349
  4. The lack and overexpression of HMGB1 affect certain aspects of plant growth and stress tolerance and it has a marked impact on the transcriptome. PMID: 18822296
Subcellular Location
Nucleus. Note=Displays a highly dynamic speckle distribution pattern in interphase chromatin but does not associate with mitotic chromosomes.
Protein Families
HMGB family
Tissue Specificity
Expressed in cotyledons, roots, stems, leaves and flowers (excluding pedicels).
Database Links

KEGG: ath:AT3G51880

STRING: 3702.AT3G51880.2

UniGene: At.27677

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