Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
ALS3; ALD8; Agglutinin-like protein 3; 3D9 antigen; Adhesin 3
Species
Candida albicans (Yeast)
Expression Region
918-1119aa
Target Protein Sequence
VSVETESSTVTTAQTNPSVPTTESEVVFTTKGNNENGPYESPSTNVKSSMDENSEFTTSTAASTSTDIENETIATTGSVEASSPIISSSADETTTVTTTAESTSVIEQPTNNNGGGKAPSATSSPSTTTTANNDSVITGTTSTNQSQSQSQYNSDTQQTTLSQQMTSSLVSLHMLTTFDGSGSVIQHSTWLCGLITLLSLFI
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Tag Info
N-terminal 10xHis-B2M-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
The recombinant Candida albicans ALS3 protein is a fusion protein consists of the Candida albicans ALS3 protein (918-1119aa) partnered with the N-terminal 10xHis-B2M tag and C-terminal Myc tag. It was produced in the E.coli. This recombinant ALS3 protein's purity is greater than 90% determined by SDS-PAGE. After electrophoresis, there is a 38 kDa protein band presented on the gel.
Candida albicans is part of the normal human flora, and it grows on mucosal surfaces in healthy individuals. Resistance caused by the formation of the Candida albicans (C. albicans) biofilm is one of the main reasons for antifungal therapy failure. Thus, it is important to find indicators that predict C. albicans biofilm formation to provide evidence for the early prevention and treatment of the C. albicans biofilms. ALS3 as a member of the agglutinin-like sequence (ALS) gene family, it was differentially expressed in free, antifungal, drug-sensitive C. albicans. A larger number of tandem repeats is associated with increased adherence when different-size alleles are expressed in C. albicans, but not when they are expressed in S. cerevisiae. ALS3 mediates adherence to diverse host substrates. ALS3 also binds to host cell ferritin and enables C. albicans to utilize this protein as a source of iron. Because of its multiple functions and its high expression level in vivo, Als3 is a promising target for vaccines that induce protective cell-mediated and antibody responses.
