Recombinant Chicken Protein Wnt-1 (WNT1)

Code CSB-YP838596CH
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP838596CH
MSDS
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Source E.coli
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Code CSB-EP838596CH-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP838596CH
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP838596CH
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
WNT1
Uniprot No.
Alternative Names
WNT1; WNT-1; Protein Wnt-1
Species
Gallus gallus (Chicken)
Expression Region
1-330
Target Protein Sequence
WWGIVNVASS TNLLTDSGSV QLVLEPGLQL LSRKQRRLIR QNPGILHSVS AGLQSAVREC QWQFRNRRWN CPTSQGPNIF GKIVNRGCRE TAFIFAITSA GVTHSVARSC SEGSIESCTC DYRRRGPGGP DWHWGGCSDN IDFGRLFGRE FVDSSEKGRD LRFLMNLHNN EAGRMTVFSE MRQECKCHGM SGSRTVRTCW MRLPTFRAVG DVLKDRFDGA SRVIYGNKGS NRASRVELHH LEPENPAHKP PSPHDLVYFE KSPNFCTYSG KMGTAGTAGR ACNSSSPGLD GCELLCCGRG FRTRTQRVTE RCNCTFHWCC HVSCLNCTNT
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Ligand for members of the frizzled family of seven transmembrane receptors. Acts in the canonical Wnt signaling pathway by promoting beta-catenin-dependent transcriptional activation. Developmental protein that promotes cell proliferation in the developing spinal cord. Has a role in osteoblast function, bone development and bone homeostasis.
Gene References into Functions
  1. Overexpression of PORCN enhances the ability of WLS to promote WNT1 trafficking to the cell surface as well as secretion, but decreases the ability of WLS to activate WNT1 signaling in target cell. PMID: 27492485
  2. Axud1 integrates Wnt signaling with transcriptional inputs to endow the neural crest with its unique molecular signature PMID: 26256212
  3. the Wnt amino acid residues required for recognition and palmitoylation by PORCN PMID: 25451226
  4. Monoclonal antibody against chick Wnt1 recognizes palmit(e)oyl-modified Wnt1 PMID: 22046319
  5. Wnt1 and Wnt8b are expressed in the rostral ectoderm of chick embryo and have roles in anterior patterning of the neural plate PMID: 14991722
  6. c-Myb but not v-Myb is sensitive to Wnt-1-induced protein degradation PMID: 15308626
  7. response to a Wnt or BMP signal during transformation of a neural tube cell into a migratory neural crest cell. PMID: 16135532
  8. Sustained reciprocal interaction between Wnt1 and FGF8 signaling is essential to maintain isthmic identity at the midbrain-hindbrain boundary. PMID: 17383629
  9. Studied Wnt1 expression patterns in chick embryo nervous system PMID: 7577679

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Subcellular Location
Secreted, extracellular space, extracellular matrix. Secreted.
Protein Families
Wnt family
Database Links

KEGG: gga:396160

UniGene: Gga.683

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