Purity
Greater than 90% as determined by SDS-PAGE.
Research Area
Cell Biology
Alternative Names
PRDX1; TDPX2Peroxiredoxin-1; EC 1.11.1.15; Thioredoxin peroxidase 2; TPX-2
Species
Cricetulus griseus (Chinese hamster) (Cricetulus barabensis griseus)
Expression Region
2-199aa
Target Protein Sequence
SSGNAKIGYPAPNFKATAVMPDGQFRDICLSEYRGKYVVFFFYPLDFTFVCPTEIIAFSDRAEEFKKLNCQVIGASVDSHFCHLAWINTPKKQGGLGPMNIPLVSDPKRTIAQDYGVLKADEGISFRGLFIIDDKGILRQITINDLPVGRSVDEILRLVQAFQFTDKHGEVCPAGWKPGSDTIKPDVQKSKEYFSKQK
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
A DNA corresponding to 2-199aa of Cricetulus griseus Peroxiredoxin-1 (PRDX1) was fused with an N-terminal 6xHis-tag and then expressed in E.coli. The resulting protein is a Recombinant Full-length pf Mature Cricetulus griseus PRDX1. This PRDX1 protein underwent the validation of SDS-PAGE and LC-MS/MS Analyses. The purity of the protein is over 90%. Its predicted molecular weight is 26.1 kDa. In addition to being an immunogen for antibody synthesis, this recombinant PRDX1 may also find uses in the studies of PRDX1-involved cell biology. In-stock PRDX1 proteins are available.
PRDX1 belongs to the superfamily of small non-seleno peroxidases and exerts a peroxide-scavenging function. In addition, PRDX1 also orchestrates redox-regulated signaling in a ROS dose-dependent manner through the oxidation status of its peroxidative cysteine Cys52. The peroxidative cysteine Cys52 of PRDX1 acts as a sensor in ROS signaling. PRDX1 promotes both MKP-1 and MKP-5 activity under normal ROS homeostasis. Upon ROS increasing, PRDX1 becomes over-oxidized and less available to bind to MKP-1, eliciting MKP-1 oxidation-induced oligomerization and inactivity toward p38MAPKα, thus facilitating apoptosis.