Recombinant Danio rerio Insulin gene enhancer protein isl-1 (isl1)

Code CSB-YP011846DIL
MSDS
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Source Yeast
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Code CSB-EP011846DIL
MSDS
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Source E.coli
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Code CSB-EP011846DIL-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP011846DIL
MSDS
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Source Baculovirus
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Code CSB-MP011846DIL
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
isl1
Uniprot No.
Alternative Names
isl1; isl-1; si:ch211-219f7.1; Insulin gene enhancer protein isl-1; Islet-1
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Expression Region
1-349
Target Protein Sequence
MGDMGDPPKK KRLISLCVGC GNQIHDQYIL RVSPDLEWHA ACLKCAECNQ YLDESCTCFV RDGKTYCKRD YIRLYGIKCA KCNIGFSKND FVMRARSKVY HIECFRCVAC SRQLIPGDEF ALREDGLFCR ADHDVVERAT MGAGDPLSPL HPARPLQMAA EPISARQPAL RPHVHKQPEK TTRVRTVLNE KQLHTLRTCY NANPRPDALM KEQLVEMTGL SPRVIRVWFQ NKRCKDKKRS ILMKQLQQQQ PNDKTNIQGM TGTPMVATSP ERHDGGLQAN QVEVQSYQPP WKVLSDFALQ SDIDQPAFQQ LVNFSEGGPG SNSTGSEVAS MSSQLPDTPN SMVASPIEA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
DNA-binding transcriptional activator. Recognizes and binds to the consensus octamer binding site 5'-ATAATTAA-3' in promoter of target genes. Plays a fundamental role in the gene regulatory network essential for retinal ganglion cell (RGC) differentiation. May be involved in subtype specialization of primary motoneurons. May bind to insulin gene enhancer sequences. Essential for heart development.
Gene References into Functions
  1. ISL1 is a major susceptibility gene for bladder exstrophy and a regulator of urinary tract development. PMID: 28176844
  2. Nkx2-5 and nkx2.7 genes restrict proliferation of heart field progenitors in the outflow tract, delimit the number of progenitors at the venous pole and pattern the sinoatrial node acting through Isl1 repression. PMID: 29361575
  3. Arx contributes to patterning in the prethalamic region, while Isl1 is required for differentiation of prethalamic dopaminergic neurons. PMID: 22728160
  4. Ajuba plays a central role in regulating the second heart field during heart development by linking retinoic acid signaling to the function of Isl1, a key transcription factor in cardiac progenitor cells. PMID: 22771034
  5. Isl1 is required for the selective outgrowth of the peripheral axons of Rohon-beard neurons. PMID: 21108318
  6. prdm1a Regulates sox10 and islet1 in the development of neural crest and Rohon-Beard sensory neurons. PMID: 20836130
  7. By direct comparison of the upstream flanking regions of the zebrafish and human isl1 genes, we identified another highly conserved noncoding element. PMID: 15680372
  8. primary motoneuron subtypes are likely to be specified by factors that act in parallel to or upstream of islet1 and islet2 PMID: 16672347
  9. Nkx6 proteins regulate MiP motorneuron development at least in part by maintaining the islet1 expression that is required both to promote the MiP subtype and to suppress interneuron development. PMID: 17376808

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Subcellular Location
Nucleus.
Database Links
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