Recombinant Danio rerio Protein Wnt-1 (wnt1)

Code CSB-YP328457DIL
MSDS
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Source Yeast
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Code CSB-EP328457DIL
MSDS
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Source E.coli
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Code CSB-EP328457DIL-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP328457DIL
MSDS
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Source Baculovirus
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Code CSB-MP328457DIL
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
wnt1
Uniprot No.
Alternative Names
wnt1; int-1; wnt-1; Protein Wnt-1
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Expression Region
20-370
Target Protein Sequence
S LTGTGAVNNS GRWWGIVNVA SSGNLLTNSK NVQLVLDPSL ALLSRRQRKL IRQNPGILHA IAAGLHTAIK ECKWQFRNRR WNCPTTHSPN VFGKIVNRGC RETAFVFAIT SAGVTHAVAR SCSEGAIESC TCDYRRRGPG GPDWHWGGCS DNVEFGRMFG REFVDSSERG RDLRYLTNLH NNEAGRMTVA SEMQQECKCH GMSGSCTVRT CWMRLPSFRL VGDYLKDRFD GASRVVYANK GSNRASHRAD PRHLEPENPA HKLPSSRDLV YFEKSPNFCS YNGKTGTHGT SGRTCNSSSP ALDGCELLCC GRGYKTRMEQ VTERCHCTFH WCCHVSCLNC TSTQTVHQCL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Ligand for members of the frizzled family of seven transmembrane receptors. Acts in the canonical Wnt signaling pathway by promoting beta-catenin-dependent transcriptional activation. Involved in neurogenesis. Performs a partially redundant function with wnt10b in the formation of the midbrain-hindbrain boundary (MHB) organizer. In the hindbrain, mediates lateral inhibition of boundary cell specification, probably via up-regulation of proneural and Delta gene expression in non-boundary cells; localized expression of wnt1 in boundary cells is maintained via rfng-mediated modulation of Notch activity.
Gene References into Functions
  1. analysis of differential role of Axin RGS domain function in Wnt signaling during anteroposterior patterning and maternal axis formation PMID: 22957046
  2. wnt1 and wnt10b are required to maintain threshold levels of Pax2.1 and Fgf8 at the midbrain-hindbrain boundary. PMID: 12591239
  3. novel role for Wnt/beta-catenin signalling in determining endocardial cell fate PMID: 14534590
  4. In zebrafish embryos lacking Wnt3a, Wnt1 and Wnt10b, the expression of engrailed orthologs, pax2a and fgf8 is not maintained after mid-somitogenesis PMID: 15147762
  5. two Dvl-associated paralogs, Dpr1 and Dpr2, participate in distinct Wnt-dependent developmental processes PMID: 15539487
  6. Wnt/Axin/beta-catenin pathway has a role in ventral CNS development PMID: 15539488
  7. The boundary and roof plate expression of wnt1 each contribute to upregulation of proneural and delta gene expression and neurogenesis in non-boundary regions. PMID: 15659486
  8. Epistatic analyses suggest a possible genetic interaction between Wnt/beta-catenin and Myostatin in regulation of slow and fast twitch muscle myofibrillogenesis PMID: 19517013

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Subcellular Location
Secreted, extracellular space, extracellular matrix. Secreted.
Protein Families
Wnt family
Tissue Specificity
In the embryo, expressed in the developing central nervous system (CNS), with an expression pattern very similar to that of wnt10b. During somitogenesis, expressed strongly in hindbrain boundary and roof plate cells.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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