Recombinant Dog NADH-cytochrome b5 reductase 3 (CYB5R3)

Code CSB-YP607302DO
MSDS
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Source Yeast
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Code CSB-EP607302DO
MSDS
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Source E.coli
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Code CSB-EP607302DO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP607302DO
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP607302DO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CYB5R3
Uniprot No.
Alternative Names
CYB5R3; C5BR; DIA1; NADH-cytochrome b5 reductase 3; B5R; Cytochrome b5 reductase; EC 1.6.2.2; Diaphorase-1) [Cleaved into: NADH-cytochrome b5 reductase 3 membrane-bound form; NADH-cytochrome b5 reductase 3 soluble form]
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Expression Region
2-301
Target Protein Sequence
GAQLSTLGH VVLSPVWFLY NLLMKLFQRS TPAITLESPD IKYPLRLIDK EVINHDTRRF RFALPSPQHI LGLPVGQHIY LSARIDGNLV IRPYTPVSSD DDKGFVDLVI KVYFKDTHPK FPAGGKMSQY LESMKIGDTI EFRGPNGLLV YQGKGKFAIR PDKKSNPIIK TVKSVGMIAG GTGITPMLQV IRAIIKDPHD PTVCHLLFAN QTEKDILLRP ELEELRNEHS ARFKLWYTVD KAPEAWDYSQ GFVNEEMIRD HLPPPEEEPL ILMCGPPPMI QYACLPNLDR VGHPKERCFA F
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Desaturation and elongation of fatty acids, cholesterol biosynthesis, drug metabolism, and, in erythrocyte, methemoglobin reduction.
Gene References into Functions
  1. All Doberman Pinschers in this study were homozygous for CYB5R3 729G, which could contribute to this breed's apparent predisposition to sulfonamide hypersensitivity; the study also showed that CYB5R3 729G does not alter sulfamethoxazole detoxification capacity. PMID: 29336038
  2. Familial congenital methemoglobinemia seen in a Pomeranian dog family was caused by congenital NADH-cytochrome b5 reductase deficiency due to a missense variant, c.580A>C, in the CYB5R3 gene. PMID: 29356095
Subcellular Location
Endoplasmic reticulum membrane; Lipid-anchor; Cytoplasmic side. Mitochondrion outer membrane; Lipid-anchor; Cytoplasmic side.
Protein Families
Flavoprotein pyridine nucleotide cytochrome reductase family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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