CYB5R3

CYB5R3 encodes cytochrome b5 reductase 3, a flavoprotein also known as methemoglobin reductase. It catalyzes the NADH-dependent reduction of cytochrome b5, a critical step in fatty acid desaturation, cholesterol biosynthesis, and drug metabolism. This enzyme maintains hemoglobin in its functional ferrous state, preventing methemoglobinemia.
CYB5R3 deficiency causes congenital methemoglobinemia types I and II, with neurological complications in the latter. It interacts with cytochromes P450 in hepatic drug metabolism pathways. Current research explores its role in cancer cell redox balance, though no targeted drugs are approved; small-molecule activators are under preclinical evaluation for metabolic disorders.

Popular Product:

CYB5R3 Antibody (CSB-PA02655A0Rb)

Validated Data

Western blot
All lanes: CYB5R3 antibody at 2µg/ml + 293T whole cell lysate
Secondary
Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 35, 32, 39 kDa
Observed band size: 32 kDa

Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA02655A0Rb at dilution of 1:100

Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA02655A0Rb at dilution of 1:100

Immunofluorescent analysis of Hela cells using CSB-PA02655A0Rb at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Human NADH-cytochrome b5 reductase 3(CYB5R3) ELISA kit (CSB-EL006320HU)

Validated Data

Code: CSB-EL006320HU

Size: 96T,5×96T,10×96T

Sensitivity: 6.25pg/ml

Detection Range: 25 pg/ml-1600 pg/ml

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

The following CYB5R3 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.

CYB5R3 Antibodies

CYB5R3 Proteins

CYB5R3 ELISA Kits

CYB5R3 Antibodies

CYB5R3 Antibodies for Homo sapiens (Human)

Code	Product Name	Species Reactivity	Application
CSB-PA02655A0Rb	CYB5R3 Antibody	Human	ELISA, WB, IHC, IF
CSB-PA006320GA01HU	CYB5R3 Antibody	Human,Mouse,Rat	ELISA,WB,IHC
CSB-PA178677	CYB5R3 Antibody	Human	ELISA,WB,IHC
CSB-PA001856	CYB5R3 Antibody	Human	WB, IHC, ELISA

CYB5R3 Antibodies for Rattus norvegicus (Rat)

Code	Product Name	Species Reactivity	Application
CSB-PA006320XA01RA	Cyb5r3 Antibody	Rattus norvegicus (Rat)	ELISA, WB (ensure identification of antigen)

CYB5R3 Proteins

CYB5R3 Proteins for Sus scrofa (Pig)

Code	Product Name	Source
CSB-YP006320PI CSB-EP006320PI CSB-BP006320PI CSB-MP006320PI CSB-EP006320PI-B	Recombinant Pig NADH-cytochrome b5 reductase 3 (CYB5R3)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CYB5R3 Proteins for Rattus norvegicus (Rat)

Code	Product Name	Source
CSB-YP006320RA CSB-EP006320RA CSB-BP006320RA CSB-MP006320RA CSB-EP006320RA-B	Recombinant Rat NADH-cytochrome b5 reductase 3 (Cyb5r3)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CYB5R3 Proteins for Bos taurus (Bovine)

Code	Product Name	Source
CSB-YP006320BO CSB-EP006320BO CSB-BP006320BO CSB-MP006320BO CSB-EP006320BO-B	Recombinant Bovine NADH-cytochrome b5 reductase 3 (CYB5R3)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CYB5R3 Proteins for Homo sapiens (Human)

Code	Product Name	Source
CSB-YP006320HU CSB-EP006320HU CSB-BP006320HU CSB-MP006320HU CSB-EP006320HU-B	Recombinant Human NADH-cytochrome b5 reductase 3 (CYB5R3)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CYB5R3 Proteins for Canis lupus familiaris (Dog) (Canis familiaris)

Code	Product Name	Source
CSB-YP607302DO CSB-EP607302DO CSB-BP607302DO CSB-MP607302DO CSB-EP607302DO-B	Recombinant Dog NADH-cytochrome b5 reductase 3 (CYB5R3)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CYB5R3 Proteins for Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)

Code	Product Name	Source
CSB-YP720221MOV CSB-EP720221MOV CSB-BP720221MOV CSB-MP720221MOV CSB-EP720221MOV-B	Recombinant Macaca fascicularis NADH-cytochrome b5 reductase 3 (CYB5R3)	Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CYB5R3 Proteins for Mus musculus (Mouse)

Code	Product Name	Source
CSB-YP887597MO CSB-BP887597MO CSB-MP887597MO CSB-EP887597MO-B	Recombinant Mouse NADH-cytochrome b5 reductase 3 (Cyb5r3)	Yeast Baculovirus Mammalian cell In Vivo Biotinylation in E.coli

CYB5R3 ELISA Kit

CYB5R3 ELISA Kit for Homo sapiens (Human)

Code	Product Name	Sample Types	Sensitivity
CSB-EL006320HU	Human NADH-cytochrome b5 reductase 3(CYB5R3) ELISA kit	serum, plasma, tissuehomogenates, cell lysates.	6.25pg/ml

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