Recombinant Drosophila melanogaster E3 ubiquitin-protein ligase Nedd-4 (Nedd4), partial

Code CSB-YP894280DLU
MSDS
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Source Yeast
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Code CSB-EP894280DLU
MSDS
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Source E.coli
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Code CSB-EP894280DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP894280DLU
MSDS
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Source Baculovirus
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Code CSB-MP894280DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Nedd4
Uniprot No.
Alternative Names
Nedd4; CG7555; E3 ubiquitin-protein ligase Nedd-4; DNedd4; EC 2.3.2.26; HECT-type E3 ubiquitin transferase NEDD4
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Essential E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Down-regulates Notch/N signaling pathway by promoting Notch ubiquitination, endocytosis and degradation.
Gene References into Functions
  1. miR-1-mediated regulation of Nedd4/Nedd4L expression may serve to broadly modulate the trafficking or degradation of Nedd4/Nedd4L substrates in the heart PMID: 28246214
  2. Nedd4Lo destabilizes Amph in muscles, leading to impaired T-tubule formation and muscle function. PMID: 26823013
  3. NEDD4 controls the Hippo pathway leading to coordinated cell proliferation and apoptosis PMID: 25692647
  4. data in two evolutionarily distant model organisms strongly suggest that Nedd4 is a modifier of alpha-synuclein pathobiology and thus a potential target for neuroprotective therapies PMID: 24388974
  5. Nedd4, another E3 ubiquitin ligase that may have a role in PD, is functionally related to Sep4 and could be involved in regulating Sep4 subcellular localization/trafficking. PMID: 22562816
  6. splice isoforms of the same dNedd4 gene can lead to opposite effects on neuromuscular synaptogenesis PMID: 22110599
  7. Nedd4 regulates endocytosis of notch and suppresses its ligand-independent activation. PMID: 15620649

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Subcellular Location
Cytoplasm.
Tissue Specificity
Ubiquitously expressed.
Database Links

KEGG: dme:Dmel_CG42279

STRING: 7227.FBpp0289746

UniGene: Dm.988

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