Recombinant Drosophila melanogaster GTP-binding protein Rheb homolog (Rheb)

Code CSB-YP895638DLU
MSDS
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Source Yeast
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Code CSB-EP895638DLU
MSDS
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Source E.coli
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Code CSB-EP895638DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP895638DLU
MSDS
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Source Baculovirus
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Code CSB-MP895638DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
Rheb; CG1081; GTP-binding protein Rheb homolog
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-179
Target Protein Sequence
MPTKERHIAM MGYRSVGKSS LCIQFVEGQF VDSYDPTIEN TFTKIERVKS QDYIVKLIDT AGQDEYSIFP VQYSMDYHGY VLVYSITSQK SFEVVKIIYE KLLDVMGKKY VPVVLVGNKI DLHQERTVST EEGKKLAESW RAAFLETSAK QNESVGDIFH QLLILIENEN GNPQEKSGC
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Binds GTP and exhibits intrinsic GTPase activity. Activates the protein kinase activity of TORC1, and thereby plays a role in the regulation of apoptosis. Stimulates the phosphorylation of S6K through activation of TORC1 signaling. May also have a role in activating TORC2 signaling.
Gene References into Functions
  1. Rheb overexpression in the central brain neurons of flies causes not only morphological phenotypes, but behavioral and aging phenotypes that may mirror symptoms of tuberous sclerosis complex PMID: 23028662
  2. Rheb promotes cell growth as a component of the insulin/TOR signaling network. PMID: 12766776
  3. Rheb is a direct target of the tuberous sclerosis suppressor proteins. PMID: 12771962
  4. dRheb is required for both cell growth (increase in mass) and cell cycle progression PMID: 12893813
  5. Rheb-TOR signaling controls S2 cell growth by promoting ribosome production and protein synthesis, not by direct effects on the import of amino acids or glucose. PMID: 17371599

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Subcellular Location
Endomembrane system; Lipid-anchor; Cytoplasmic side. Golgi apparatus membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, cytosol. Endoplasmic reticulum membrane; Lipid-anchor; Cytoplasmic side.
Protein Families
Small GTPase superfamily, Rheb family
Database Links

KEGG: dme:Dmel_CG1081

STRING: 7227.FBpp0078342

UniGene: Dm.11577

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