Recombinant Escherichia coli Transcriptional regulatory protein phoP (phoP)

Just like other recombinant proteins, the production of this recombinant E.coli phoP protein began with appropriate cDNA and PCR methods, and then the phoP expression plasmids were built. Following sequence determination of the constructs, plasmids were transformed into Mammalian cell for the expression of the recombinant E.coli phoP protein. N-terminal 10xHis tag & C-terminal Myc tag was used in the process. And we finally get the protein of interest with purity of 90%+.

phoP is a gene providing an instruction of making a protein called transcriptional regulatory protein PhoP (also called Phop) widely presented in Gram-negative bacteria, such as Escherichia coli (E.coli). Phop is a virulence regulator conserved in both pathogenic and non-pathogenic Enterobacteriaceae. PhoP plays an important role as a transcriptional regulator in the two-component phoP/phoQ regulatory system with DNA-binding transcription activator activity. Moreover, This protein has phosphorelay response regulator activity and also can bind to transcription cis-regulatory region.