Recombinant Escherichia coli Transcriptional regulatory protein phoP (phoP)

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Code CSB-MP326097ENV
Size $660
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of CSB-MP326097ENV could indicate that this peptide derived from Mammalian Cell-expressed Escherichia coli (strain K12) phoP.
  • Based on the SEQUEST from database of Mammalian Cell host and target protein, the LC-MS/MS Analysis result of CSB-MP326097ENV could indicate that this peptide derived from Mammalian Cell-expressed Escherichia coli (strain K12) phoP.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
phoP
Uniprot No.
Research Area
others
Alternative Names
phoP; b1130; JW1116; Transcriptional regulatory protein PhoP
Species
Escherichia coli (strain K12)
Source
Mammalian cell
Expression Region
1-223aa
Target Protein Sequence
MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHIPDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKPFHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMETLIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGYLFELR
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
29.5kDa
Protein Length
Full Length
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

Just like other recombinant proteins, the production of this recombinant E.coli phoP protein began with appropriate cDNA and PCR methods, and then the phoP expression plasmids were built. Following sequence determination of the constructs, plasmids were transformed into Mammalian cell for the expression of the recombinant E.coli phoP protein. N-terminal 10xHis tag & C-terminal Myc tag was used in the process. And we finally get the protein of interest with purity of 90%+.

phoP is a gene providing an instruction of making a protein called transcriptional regulatory protein PhoP (also called Phop) widely presented in Gram-negative bacteria, such as Escherichia coli (E.coli). Phop is a virulence regulator conserved in both pathogenic and non-pathogenic Enterobacteriaceae. PhoP plays an important role as a transcriptional regulator in the two-component phoP/phoQ regulatory system with DNA-binding transcription activator activity. Moreover, This protein has phosphorelay response regulator activity and also can bind to transcription cis-regulatory region.

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Target Background

Function
Member of the two-component regulatory system PhoP/PhoQ involved in adaptation to low Mg(2+) environments and the control of acid resistance genes. In low periplasmic Mg(2+), PhoQ phosphorylates PhoP, resulting in the expression of PhoP-activated genes (PAG) and repression of PhoP-repressed genes (PRG). In high periplasmic Mg(2+), PhoQ dephosphorylates phospho-PhoP, resulting in the repression of PAG and may lead to expression of some PRG. Mediates magnesium influx to the cytosol by activation of MgtA. Promotes expression of the two-component regulatory system rstA/rstB and transcription of the hemL, mgrB, nagA, slyB, vboR and yrbL genes.
Gene References into Functions
  1. findings directly link the PhoQ/PhoP two-component system to bacterial osmosensing PMID: 29183977
  2. Both MicA and GcvB bind phoPQ mRNA in vivo and in vitro around the translation initiation region of phoP. Binding of either small RNA is sufficient to inhibit ribosome binding and induce mRNA degradation PMID: 23300478
  3. Taken together, these results demonstrate that a decrease in the oxidizing activity of the periplasm stimulates PhoQ/PhoP and may reveal a new input stimulus for this important two-component system. PMID: 22267510
  4. MicA directly pairs with phoPQ mRNA in the translation initiation region of phoP and presumably inhibits translation by competing with ribosome binding. PMID: 20345657
Subcellular Location
Cytoplasm.
Database Links
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