phoP Antibody

Datasheet

Code	CSB-PA326097ZA01ENV
Size	US$299
Image	Western Blot Positive WB detected in Recombinant protein All lanes: phoP antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 30.5 kDa Observed band size: 37 kDa
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Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) phoP Polyclonal antibody

Uniprot No.

P23836

Target Names

phoP

Alternative Names

phoP antibody; b1130 antibody; JW1116 antibody; Transcriptional regulatory protein PhoP antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli

Immunogen

Recombinant Escherichia coli Transcriptional regulatory protein phoP (phoP) (1-223AA)

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Protein A/G

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The production of the phoP polyclonal antibody involves a methodical approach. It starts with the repeated immunization of a rabbit using recombinant Escherichia coli transcriptional regulatory protein phoP (1-223aa), continuing until the desired antibody titer is achieved. Following this, the rabbit's blood is collected, and the antibodies are carefully purified from the serum using protein A/G. The resulting phoP antibody undergoes rigorous evaluation through ELISA and WB applications, confirming its specific reactivity with the Escherichia coli phoP protein.

Escherichia coli phoP acts as a transcriptional regulator that controls the expression of genes involved in phosphate sensing, uptake, and utilization. Its primary role is to help the bacterium adapt to changes in phosphate availability in its environment and to ensure that it can efficiently acquire and utilize phosphate for various cellular processes.

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Target Background

Function

Member of the two-component regulatory system PhoP/PhoQ involved in adaptation to low Mg(2+) environments and the control of acid resistance genes. In low periplasmic Mg(2+), PhoQ phosphorylates PhoP, resulting in the expression of PhoP-activated genes (PAG) and repression of PhoP-repressed genes (PRG). In high periplasmic Mg(2+), PhoQ dephosphorylates phospho-PhoP, resulting in the repression of PAG and may lead to expression of some PRG. Mediates magnesium influx to the cytosol by activation of MgtA. Promotes expression of the two-component regulatory system rstA/rstB and transcription of the hemL, mgrB, nagA, slyB, vboR and yrbL genes.

Gene References into Functions

findings directly link the PhoQ/PhoP two-component system to bacterial osmosensing PMID: 29183977
Both MicA and GcvB bind phoPQ mRNA in vivo and in vitro around the translation initiation region of phoP. Binding of either small RNA is sufficient to inhibit ribosome binding and induce mRNA degradation PMID: 23300478
Taken together, these results demonstrate that a decrease in the oxidizing activity of the periplasm stimulates PhoQ/PhoP and may reveal a new input stimulus for this important two-component system. PMID: 22267510
MicA directly pairs with phoPQ mRNA in the translation initiation region of phoP and presumably inhibits translation by competing with ribosome binding. PMID: 20345657

Subcellular Location

Cytoplasm.

Database Links

KEGG: ecj:JW1116

STRING: 316385.ECDH10B_1202

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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phoP Antibody

Product Details

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Customer Reviews and Q&A

Target Background