Purity
Greater than 90% as determined by SDS-PAGE.
Alternative Names
vacA; HP_0887; Vacuolating cytotoxin autotransporter [Cleaved into: Vacuolating cytotoxin; Vacuolating cytotoxin translocator]
Species
Helicobacter pylori (strain ATCC 700392 / 26695) (Campylobacter pylori)
Expression Region
1018-1290aa
Target Protein Sequence
KYEKPTNVWANAIGGTSLNNGGNASLYGTSAGVDAYLNGEVEAIVGGFGSYGYSSFNNQANSLNSGANNTNFGVYSRIFANQHEFDFEAQGALGSDQSSLNFKSALLRDLNQSYNYLAYSAATRASYGYDFAFFRNALVLKPSVGVSYNHLGSTNFKSNSNQVALKNGSSSQHLFNASANVEARYYYGDTSYFYMNAGVLQEFANFGSSNAVSLNTFKVNAAHNPLSTHARVMMGGELKLAKEVFLNLGFVYLHNLISNIGHFASNLGMRYSF
Note: The complete sequence including tag
sequence, target protein sequence and linker sequence could be provided upon request.
Tag Info
N-terminal 6xHis-B2M-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that
we have in stock, however, if you have any special requirement for the format, please remark your
requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the
glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer,
6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw
cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature
and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized
form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description
Recombinant Helicobacter pylori Vacuolating cytotoxin autotransporter(vacA) is a partial-length protein derived from E.coli. The expression region of this protein is the 1018-1290aa of Helicobacter pylori vacA. It carries an N-terminal 6xHis-B2M-tag and has a calculated molecular weight of 43.6 kDa. Its purity is greater than 90% as determined by SDS-PAGE and its identity was validated by the LC-MS/MS Analysis. This recombinant vacA protein may be used to generate specific antibodies and in microbiology research.
VacA is one of the predominant protein toxins secreted by the type V auto-transport secretion system of the Helicobacter pylori (H. pylori). The H. pylori is a pathogenic bacterium that potentially causes peptic ulcers of the human stomach even gastric cancer. VacA is important for H. pylori colonization in vivo and has the capacity of causing "vacuole"-like membrane vesicles in the cytoplasm of gastric cells. Besides, it has other effects on target cells, including disturbing mitochondrial functions, stimulating apoptosis, and blocking T cell proliferation.
