Recombinant Human 2-oxoglutarate dehydrogenase, mitochondrial

Code CSB-YP016307HU
MSDS
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Source Yeast
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Code CSB-EP016307HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP016307HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP016307HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
OGDH
Uniprot No.
Research Area
Metabolism
Alternative Names
2 oxoglutarate dehydrogenase; 2 oxoglutarate dehydrogenase complex component E1; 2 oxoglutarate dehydrogenase mitochondrial; 2-oxoglutarate dehydrogenase; 2-oxoglutarate dehydrogenase complex component E1; AKGDH; Alpha ketoglutarate dehydrogenase ; Alpha-ketoglutarate dehydrogenase; E1k; mitochondrial; ODO1_HUMAN; OGDC; OGDC E1; OGDC-E1; OGDH; Oxoglutarate (alpha ketoglutarate) dehydrogenase (lipoamide); Oxoglutarate decarboxylase; Oxoglutarate dehydrogenase (lipoamide); Oxoglutarate dehydrogenase (succinyl transferring)
Species
Homo sapiens (Human)
Expression Region
41-427aa
Target Protein Sequence
SAPVAAEPFLSGTSSNYVEEMYCAWLENPKSVHKSWDIFFRNTNAGAPPGTAYQSPLPLSRGSLAAVAHAQSLVEAQPNVDKLVEDHLAVQSLIRAYQIRGHHVAQLDPLGILDADLDSSVPADIISSTDKLGFYGLDESDLDKVFHLPTTTFIGGQESALPLREIIRRLEMAYCQHIGVEFMFINDLEQCQWIRQKFETPGIMQFTNEEKRTLLARLVRSTRFEEFLQRKWSSEKRFGLEGCEVLIPALKTIIDKSSENGVDYVIMGMPHRGRLNVLANVIRKELEQIFCQFDSKLEAADEGSGDVKYHLGMYHRRINRVTDRNITLSLVANPSHLEAADPVVMGKTKAEQFYCGDTEGKKVRPRERRARQIVKAPCSSMEFRSPT
Mol. Weight
58.96kD
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
2-oxoglutarate dehydrogenase (E1) component of the 2-oxoglutarate dehydrogenase complex (OGDHC), which mediates the decarboxylation of alpha-ketoglutarate. The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A.
Gene References into Functions
  1. PIK3CA mutant cancer cells require PIK3CA but also require the expression of the TCA cycle enzyme 2-oxoglutarate dehydrogenase (OGDH). PMID: 28396387
  2. oxoglutarate dehydrogenase (OGDH) and lipoic acid synthase (LIAS), which when mutated stabilize HIF1alpha in a non-hydroxylated form. PMID: 27923773
  3. Tissue-specific expression of OGDH splice variants may thus provide a mechanism that tunes the control of the enzyme to the specialized metabolic and signalling needs of individual cell types PMID: 26936970
  4. ATP consumption is demonstrated in respiration-impaired isolated mice and in situ neuronal mitochondria from transgenic mice with dihydrolipoyl succinyltransferase deficiency. PMID: 23475850
  5. Acute inhibition of alpha-ketoglutarate dehydrogenase produces effects on calcium opposite to those in Alzheimer's disease (AD), while the chronic or long-term inhibition of alpha-KGDHC mimicked the AD-related changes in calcium. PMID: 22169199
  6. Differences in oxoglutarate dehydrogenase activity suggest that there is no excess mitochondrial capacity during maximal exercise with a small muscle mass. PMID: 21611730
  7. 2-oxoglutarate (alpha-ketoglutarate) dehydrogenase stability is regulated by the RING finger ubiquitin ligase Siah PMID: 15466852
  8. Reduction in the E2k subunit of the alpha-ketoglutarate dehydrogenase complex has effects independent of complex activity. PMID: 15649899
  9. Association with autosomal recessive DOOR syndrome not found. PMID: 17343268

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Subcellular Location
Mitochondrion matrix. Nucleus.
Protein Families
Alpha-ketoglutarate dehydrogenase family
Database Links

HGNC: 8124

OMIM: 613022

KEGG: hsa:4967

STRING: 9606.ENSP00000222673

UniGene: Hs.488181

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