Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

OGDH

Uniprot No.

Q02218

Alternative Names

2 oxoglutarate dehydrogenase; 2 oxoglutarate dehydrogenase complex component E1; 2 oxoglutarate dehydrogenase mitochondrial; 2-oxoglutarate dehydrogenase; 2-oxoglutarate dehydrogenase complex component E1; AKGDH; Alpha ketoglutarate dehydrogenase ; Alpha-ketoglutarate dehydrogenase; E1k; mitochondrial; ODO1_HUMAN; OGDC; OGDC E1; OGDC-E1; OGDH; Oxoglutarate (alpha ketoglutarate) dehydrogenase (lipoamide); Oxoglutarate decarboxylase; Oxoglutarate dehydrogenase (lipoamide); Oxoglutarate dehydrogenase (succinyl transferring)

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

41-427aa

Target Protein Sequence

SAPVAAEPFLSGTSSNYVEEMYCAWLENPKSVHKSWDIFFRNTNAGAPPGTAYQSPLPLSRGSLAAVAHAQSLVEAQPNVDKLVEDHLAVQSLIRAYQIRGHHVAQLDPLGILDADLDSSVPADIISSTDKLGFYGLDESDLDKVFHLPTTTFIGGQESALPLREIIRRLEMAYCQHIGVEFMFINDLEQCQWIRQKFETPGIMQFTNEEKRTLLARLVRSTRFEEFLQRKWSSEKRFGLEGCEVLIPALKTIIDKSSENGVDYVIMGMPHRGRLNVLANVIRKELEQIFCQFDSKLEAADEGSGDVKYHLGMYHRRINRVTDRNITLSLVANPSHLEAADPVVMGKTKAEQFYCGDTEGKKVRPRERRARQIVKAPCSSMEFRSPT
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.

Mol. Weight

59.1kDa

Protein Length

Full Length of Mature Protein of Isoform 3

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Tris-based buffer,50% glycerol

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Target Background

Function

2-oxoglutarate dehydrogenase (E1) component of the 2-oxoglutarate dehydrogenase complex (OGDHC), which mediates the decarboxylation of alpha-ketoglutarate. The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). The 2-oxoglutarate dehydrogenase complex is mainly active in the mitochondrion. A fraction of the 2-oxoglutarate dehydrogenase complex also localizes in the nucleus and is required for lysine succinylation of histones: associates with KAT2A on chromatin and provides succinyl-CoA to histone succinyltransferase KAT2A.

Gene References into Functions

PIK3CA mutant cancer cells require PIK3CA but also require the expression of the TCA cycle enzyme 2-oxoglutarate dehydrogenase (OGDH). PMID: 28396387
oxoglutarate dehydrogenase (OGDH) and lipoic acid synthase (LIAS), which when mutated stabilize HIF1alpha in a non-hydroxylated form. PMID: 27923773
Tissue-specific expression of OGDH splice variants may thus provide a mechanism that tunes the control of the enzyme to the specialized metabolic and signalling needs of individual cell types PMID: 26936970
ATP consumption is demonstrated in respiration-impaired isolated mice and in situ neuronal mitochondria from transgenic mice with dihydrolipoyl succinyltransferase deficiency. PMID: 23475850
Acute inhibition of alpha-ketoglutarate dehydrogenase produces effects on calcium opposite to those in Alzheimer's disease (AD), while the chronic or long-term inhibition of alpha-KGDHC mimicked the AD-related changes in calcium. PMID: 22169199
Differences in oxoglutarate dehydrogenase activity suggest that there is no excess mitochondrial capacity during maximal exercise with a small muscle mass. PMID: 21611730
2-oxoglutarate (alpha-ketoglutarate) dehydrogenase stability is regulated by the RING finger ubiquitin ligase Siah PMID: 15466852
Reduction in the E2k subunit of the alpha-ketoglutarate dehydrogenase complex has effects independent of complex activity. PMID: 15649899
Association with autosomal recessive DOOR syndrome not found. PMID: 17343268

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Subcellular Location

Mitochondrion matrix. Nucleus.

Protein Families

Alpha-ketoglutarate dehydrogenase family

Database Links

HGNC: 8124

OMIM: 613022

KEGG: hsa:4967

STRING: 9606.ENSP00000222673

UniGene: Hs.488181

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Code	CSB-EP016307HU
MSDS	MSDS
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human 2-oxoglutarate dehydrogenase, mitochondrial (OGDH)

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