Recombinant Human Alpha-internexin (INA)

Code CSB-YP624093HU
MSDS
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Source Yeast
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Code CSB-EP624093HU
MSDS
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Source E.coli
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Code CSB-EP624093HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP624093HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP624093HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
INA
Uniprot No.
Alternative Names
66 kDa neurofilament protein; AINX_HUMAN; Alpha Inx; Alpha-internexin; Alpha-Inx; INA; Internexin neuronal intermediate filament protein alpha ; MGC12702; NEF 5; NEF5; Neurofilament 5 (66kD); Neurofilament 5; Neurofilament 66; Neurofilament 66 tax binding protein ; Neurofilament-66; NF 66; NF-66; NF66 ; TXBP 1; TXBP1
Species
Homo sapiens (Human)
Expression Region
1-499
Target Protein Sequence
MSFGSEHYLC SSSSYRKVFG DGSRLSARLS GAGGAGGFRS QSLSRSNVAS SAACSSASSL GLGLAYRRPP ASDGLDLSQA AARTNEYKII RTNEKEQLQG LNDRFAVFIE KVHQLETQNR ALEAELAALR QRHAEPSRVG ELFQRELRDL RAQLEEASSA RSQALLERDG LAEEVQRLRA RCEEESRGRE GAERALKAQQ RDVDGATLAR LDLEKKVESL LDELAFVRQV HDEEVAELLA TLQASSQAAA EVDVTVAKPD LTSALREIRA QYESLAAKNL QSAEEWYKSK FANLNEQAAR STEAIRASRE EIHEYRRQLQ ARTIEIEGLR GANESLERQI LELEERHSAE VAGYQDSIGQ LENDLRNTKS EMARHLREYQ DLLNVKMALD IEIAAYRKLL EGEETRFSTS GLSISGLNPL PNPSYLLPPR ILSATTSKVS STGLSLKKEE EEEEASKVAS KKTSQIGESF EEILEETVIS TKKTEKSNIE ETTISSQKI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Class-IV neuronal intermediate filament that is able to self-assemble. It is involved in the morphogenesis of neurons. It may form an independent structural network without the involvement of other neurofilaments or it may cooperate with NEFL to form the filamentous backbone to which NEFM and NEFH attach to form the cross-bridges. May also cooperate with the neuronal intermediate filament protein PRPH to form filamentous networks.
Gene References into Functions
  1. Data suggest GRINL1A (GCOM1)-NMDA receptor-internexin-alpha (INA) interaction pathway may be relevant to neuroprotection. PMID: 29339073
  2. It is a neuronal marker, and has been indicated as an immunohistochemical surrogate of chromosome 1p/19q co-deletion in oligodendroglial tumors. PMID: 26233522
  3. Gonadotropinomas, null cell pituitary adenomas, and thyrotropinomas exhibit high levels of intracellular INA protein indicating neuronal transdifferentiation. PMID: 25236435
  4. It is a supportive diagnostic marker for oligodendroglial tumors with the 1p/19q co-deletion. PMID: 24197863
  5. alpha-Internexin was expressed in 53% of 350 pancreatic neuroendocrine tumors. Reduced expression of alpha-internexin was significantly associated with advanced stage metastases, recurrence and shorter overall survival. PMID: 24483152
  6. High INA expression is associated with grade II gliomas. PMID: 22890969
  7. INA may provide additional biologic information relevant to delineation of both pancreatic neuroendocrine neoplasms (NEN) tumor phenotypes and clinical behavior. PMID: 21990041
  8. the importance of alpha-internexin and NF-L in regulating the conformations of NF-M and NF-H PMID: 20213320
  9. alpha-internexin expression in neuroblastoma PMID: 12209604
  10. The discovery of alpha-internexin in the cytoplasmic inclusions implicates novel mechanisms of pathogenesis in inclusion diseeae and other neurological diseases with pathological accumulations of IFs. PMID: 15161649
  11. Abnormal neuronal intermediate filament inclusions of alpha-internexin have been identified as the pathological hallmark of neuronal intermediate filament inclusion disease PMID: 16722980
  12. Expression of the 2 markers, alpha-internexin and peripherin, in a small round cell tumor strongly favors the diagnosis of neuroblastoma. PMID: 18528283
  13. Alpha-Internexin (INA) expression appears to be a simple, reliable prognostic marker and a surrogate marker of 1p19q codeletion. PMID: 19139367

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Protein Families
Intermediate filament family
Tissue Specificity
Found predominantly in adult CNS.
Database Links

HGNC: 6057

OMIM: 605338

KEGG: hsa:9118

STRING: 9606.ENSP00000358865

UniGene: Hs.500916

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