Recombinant Human Alpha-protein kinase 1 (ALPK1), partial

In Stock
Code CSB-BP836286HU
MSDS
Size $528
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
ALPK1
Uniprot No.
Research Area
Signal Transduction
Alternative Names
Chromosome 4 kinase Lymphocyte alpha-protein kinase
Species
Homo sapiens (Human)
Source
Baculovirus
Expression Region
1017-1237aa
Target Protein Sequence
KYSKKSELWTAQETIVYLGDYLTVKKKGRQRNAFWVHHLHQEEILGRYVGKDYKEQKGLWHHFTDVERQMTAQHYVTEFNKRLYEQNIPTQIFYIPSTILLILEDKTIKGCISVEPYILGEFVKLSNNTKVVKTEYKATEYGLAYGHFSYEFSNHRDVVVDLQGWVTGNGKGLIYLTDPQIHSVDQKVFTTNFGKRGIFYFFNNQHVECNEICHRLSLTRP
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
29.9 kDa
Protein Length
Partial
Tag Info
N-terminal 10xHis-tagged and C-terminal Myc-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Serine/threonine-protein kinase that detects bacterial pathogen-associated molecular pattern metabolites (PAMPs) and initiates an innate immune response, a critical step for pathogen elimination and engagement of adaptive immunity. Specifically recognizes and binds ADP-D-glycero-beta-D-manno-heptose (ADP-Heptose), a potent PAMP present in all Gram-negative and some Gram-positive bacteria. ADP-Heptose-binding stimulates its kinase activity to phosphorylate and activate TIFA, triggering proinflammatory NF-kappa-B signaling. May be involved in monosodium urate monohydrate (MSU)-induced inflammation by mediating phosphorylation of unconventional myosin MYO9A. May also play a role in apical protein transport by mediating phosphorylation of unconventional myosin MYO1A. May play a role in ciliogenesis.
Gene References into Functions
  1. In lung and colorectal cancer tissues, the ALPK1 RNA level of the normal part was higher than that of the tumor part. these findings suggested that ALPK1 might play a vital role in cancer development. PMID: 27283888
  2. ALPK1 is a kinase that participates in the regulation of Golgi-derived TNF-alpha trafficking through myosin IIA phosphorylation in the inflammation of gout. PMID: 27169898
  3. ERN1 and ALPK1 inhibit differentiation of bi-potential tumor-initiating cells in human triple negative breast cancer. PMID: 27829216
  4. these results clearly show that ALPK1 is a master regulator of innate immunity against both invasive and extracellular gram-negative bacteria. PMID: 28222186
  5. findings suggest that among genes in a gout-susceptibility locus, not ALPK1 but ABCG2 could be important as a gout-susceptible gene PMID: 25326865
  6. single nucleotide polymorphisms in the alpha-protein kinase 1 (ALPK1) gene are associated with gout in Han Chinese Taiwanese. PMID: 23569188
  7. ALPK1 may be a susceptibility gene for chronic kidney disease in such individuals with diabetes mellitus. PMID: 23539754
  8. ALPK1 is a gout-susceptible gene involved in Monosodium urate monohydrate-induced inflammatory responses and may contribute to the development of gout by enhancing the inflammatory responses via the MAP kinase pathway PMID: 21822924
  9. Transposon-inserted Alpk1 homozygous mutant mice display severe defects in motor coordination in a series of behavioral analyses. PMID: 21208416
  10. the phosphorylation of myosin I by ALPK1 is an essential process in the apical trafficking of raft-associated sucrose-isomaltase PMID: 15883161

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Subcellular Location
Cytoplasm, cytosol. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cell projection, cilium.
Protein Families
Protein kinase superfamily, Alpha-type protein kinase family, ALPK subfamily
Tissue Specificity
Highly expressed in liver. Expressed in the optic nerve and retinal pigmented epithelium. Lower expression is observed in the macula and extramacular retina.
Database Links

HGNC: 20917

OMIM: 607347

KEGG: hsa:80216

STRING: 9606.ENSP00000177648

UniGene: Hs.652825

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