Recombinant Human Apoptotic protease-activating factor 1(APAF1),partial

Code CSB-YP001885HU
Size US$1916
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names APAF1
Uniprot No. O14727
Research Area Cancer
Alternative Names APAF 1; Apaf-1; APAF_HUMAN; Apaf1; Apoptotic peptidase activating factor 1; Apoptotic protease activating factor 1; Apoptotic protease activating factor; Apoptotic protease-activating factor 1; CED 4; CED4; KIAA0413
Species Homo sapiens (Human)
Source Yeast
Expression Region 104-415aa
Target Protein Sequence SGITSYVRTVLCEGGVPQRPVVFVTRKKLVNAIQQKLSKLKGEPGWVTIHGMAGCGKSVLAAEAVRDHSLLEGCFPGGVHWVSVGKQDKSGLLMKLQNLCTRLDQDESFSQRLPLNIEEAKDRLRILMLRKHPRSLLILDDVWDSWVLKAFDSQCQILLTTRDKSVTDSVMGPKYVVPVESSLGKEKGLEILSLFVNMKKADLPEQAHSIIKECKGSPLVVSLIGALLRDFPNRWEYYLKQLQNKQFKRIRKSSSYDYEALDEAMSISVEMLREDIKDYYTDLSILQKDVKVPTKVLCILWDMETEEVEDIL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 37.4kDa
Protein Length Partial
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis. This activation requires ATP. Isoform 6 is less effective in inducing apoptosis.
Gene References into Functions
  1. APAF1 mutations cause recurrent folate-resistant neural tube defects. PMID: 29358613
  2. Study suggests a potential underlying molecular mechanism of apoptosis inhibition via APAF1 downregulation in human neuroblastoma BE(2)C cells with miRNA36133p overexpression. PMID: 30066861
  3. Down-regulation of Apaf-1 protein and the overexpression of Cyclin D1 and AQP-5 proteins possibly contribute to an aggressive serous ovarian carcinoma with a high risk of recurrence and poor response to the first-line chemotherapy. PMID: 30072015
  4. Results suggest that low levels of Apaf-1 as an adaptor protein might be considered as a possible regulatory barrier by which differentiating cells control cell death upon rise in ROS production and cytochrome c release from mitochondria. PMID: 29395479
  5. APAF-1 was found to be reduced in non-small-cell lung cancer tissue samples along with high expression of miR-484. PMID: 28982084
  6. miR-300 regulates the cellular sensitivity to ionizing radiation through targeting p53 and apaf1 in lung cancer cells. PMID: 28895780
  7. we sought to investigate mechanisms mediated by Hsp70 acetylation in relation to apoptotic and autophagic programmed cell death. Upon stress-induced apoptosis, Hsp70 acetylation inhibits apoptotic cell death, mediated by Hsp70 association with apoptotic protease-activating factor (Apaf)-1 and apoptosis-inducing factor (AIF), key modulators of caspase-dependent and -independent apoptotic pathways, respectively PMID: 28627586
  8. Knockdown of microRNA-27a increased the expression level of Apaf-1, enhancing the formation of Apaf-1-caspase-9 complex and subsequently promoting the TRAIL-induced apoptosis in colorectal cancer stem cells. Findings suggested that knockdown of microRNA-27a in colorectal cancer stem cells by the specific antioligonucleotides was potential to reverse the chemoresistance to TRAIL. PMID: 28423356
  9. Results indicate that the apoptotic protease-activating factor 1 (Apaf-1) apoptosome activates caspase-9 in part through sequestration of the inhibitory caspase recruitment domains (CARDs) domain. PMID: 28143931
  10. Authors found significant up-regulation of miR-221 and significant down-regulation of Apaf-1 expression in LSCC tissues compared to normal nearby laryngeal tissues. Significant associations between up-regulated miR-221 and down-regulated Apaf-1 expressions and clinical stage and lymph node (LN) metastasis were found. PMID: 28453462
  11. A significant correlation between changes in the levels of expression and methylation was detected for the three apoptosis-regulatory genes (APAF1, DAPK1, and BCL2). The results suggest that methylation play an important role in the regulation of the apoptosis system genes in breast cancer. PMID: 28429233
  12. Primary cells derived from patients with diffuse large B cell lymphomas show membrane raft sequestration of the apoptosome adaptor protein, Apaf-1, which may mediate drug resistance. PMID: 27863378
  13. Loss of APAF-1 expression is associated with early recurrence in stage I-III colorectal cancer, suggesting that APAF-1 may have clinical value as a predictive marker of early recurrence. PMID: 27480415
  14. the markers Ets-1 and APAF-1 relative to p53, Ki-67 and PTEN expression in colon adenomas/polyps, were investigated. PMID: 26743285
  15. Different selenium concentrations had varying effects on BAK1 and APAF1 levels. APAF1 may play an important role in the pathogenesis of KBD PMID: 26179084
  16. miR21 is transferred from cancer-associated adipocytes (CAAs) and fibroblasts (CAFs) to the cancer cells, where it suppresses ovarian cancer apoptosis and confers chemoresistance by binding to its direct novel target, APAF1. PMID: 27021436
  17. HDAC inhibitors can induce p53 acetylation at lysine 120, which in turn enhances mitochondrion-mediated apoptosis through transcriptional up-regulation of Apaf-1. PMID: 26851285
  18. Renal APAF1 expression is increased in diabetes and diabetic nephropathy. PMID: 27141571
  19. Inhibition of Caspase-9 restricted, while Apaf-1 promoted, Chlamydia pneumoniae infection in HEp-2, HeLa, and mouse epithelial fibroblast (MEF) cells. PMID: 26290316
  20. These results suggest that CED-4 forms a complex with ced-3 mRNA and delivers it to ribosomes for translation. PMID: 26740177
  21. The auto-assembly of truncated Apaf1 molecules disappeared after several hours without any enzyme activation. PMID: 25895636
  22. cytochrome c and the WD domains of Apaf-1 interact, with bifurcated salt bridges involved in apoptosome assembly PMID: 26014357
  23. NF-kappaB response element, located close to the p53RE#1, mediates APAF1 transcriptional repression by affecting interaction between KAISO and p53 PMID: 26183023
  24. This study suggested that miR-23a, acting as an oncogenic regulator by directly targeting APAF1 in pancreatic cancer, is a useful potential biomarker in diagnosis and treatment of pancreatic cancer. PMID: 25701323
  25. Our results indicate that the APAF1, BAX, and FLASH genes not only harbor frameshift mutations but also demonstrate mutational ITH, which together might play a role in the tumorigenesis of CRC with MSI-H by affecting the apoptosis of cancer cells. PMID: 25599959
  26. a role for Apaf1 at the mitochondria PMID: 24416260
  27. APAF-1 plays an important role in iron-induced erythroid apoptosis increase in myelodysplastic syndrome PMID: 24142530
  28. The inhibitory effect of Ab42 on the apoptotic pathway is associated with its interaction with procaspase-9 and consequent inhibition of Apaf-1 apoptosome assembly PMID: 24424093
  29. Apoptosis of HGC-27 cells induced by oridonin may be associated with differential expression of Apaf-1, caspase-3 and cytochrome c, which are highly dependent upon the mitochondrial pathway PMID: 23326121
  30. Amiloride modulates glioblastoma multiforme cell radiosensitivity involving the Akt phosphorylation and the alternative splicing of APAF1. PMID: 23822711
  31. Apaf-1 conformational changes during apoptosome assembly. PMID: 23521171
  32. Data indicate that the formation of cytochrome c-Apaf-1 apoptosome and the presence of Smac are absolutely required for PSAP-induced apoptosis. PMID: 23207240
  33. Both the silencing of miR-155 and the overexpression Apaf-1 greatly increased the sensitivity of A549 cells to cisplatin. PMID: 22996741
  34. The 5' untranslated region of Apaf-1 mRNA directs translation under apoptosis conditions via a 5' end-dependent scanning mechanism. PMID: 23085065
  35. Curcumin treatment of cancer cells led to Apaf-1 upregulation both at the protein and mRNA levels. Deficiency of Apaf-1 inhibits caspase activation and cell death. PMID: 22101335
  36. APAF-1, DAPK-1 and SPARC may have a role in progression and higher tumor stage of renal cell carcinoma in North Indian population PMID: 21922274
  37. Collectively, these results identify a novel locus of apoptosomal regulation wherein MAPK signalling promotes Rsk-catalysed Apaf-1 phosphorylation and consequent binding of 14-3-3varepsilon, resulting in decreased cellular responsiveness to cytochrome c. PMID: 22246185
  38. These results suggest that, in addition to the classical cytochrome c/Apaf-1-dependent pathway of caspase-9 activation, staurosporine can induce caspase-9 activation and apoptosis independently of the apoptosome. PMID: 21659556
  39. Results show a significant inverse correlation between GLUT1 expression and Apaf-1 expression in colorectal adenocarcinomas, and patients with GLUT1 expression demonstrate poor overall survival. PMID: 21528061
  40. in the absence of nucleotide such as ATP, direct association of procaspase-9 with Apaf-1 leads to defective molecular timer, and thus, inhibits apoptosome-mediated caspase activation PMID: 21297999
  41. Expression of Apaf-1 was up-regulated in MCF-7 cells treated with 5-Aza-CdR. PMID: 19662370
  42. Hypermethylation of APAF1 gene is associated with neuroblastoma. PMID: 21104989
  43. higher expression of APAF-1 is related to an undifferentiated state in the testicular germ cell tumor (TGCT) pathway. PMID: 20977544
  44. APAF-1 expression was significantly higher in low-risk, compared to high-risk myelodysplastic syndrome, according to IPSS (P < 0.0001), FAB (P = 0.0265), and cytogenetic risk (P = 0.0134). PMID: 20345447
  45. results show that the APAF1 variants associated with risk for MDD in the Utah pedigrees are very rare in Northern European and European-American populations PMID: 19455599
  46. Studies suggest that the apoptosome contains a 1:1 Apaf-1:caspase-9 stoichiometry. PMID: 19809088
  47. A drug targeting Apaf-1 allows protection from apoptosis as well as regeneration in the course of inflammation-induced tissue injury PMID: 19675677
  48. Apaf1 WD40 repeat domains form propellar-like structures that are important for procaspase 9 binding to the CARD domain. PMID: 11864614
  49. lack of role in caspase-9 activation in Sendai virus-infected cells PMID: 12021264
  50. APAF-1 is under transcriptional regulation of E2F-1 and initiates a caspase cascade PMID: 12149244
  51. Data show that the trace amount of cytochrome c present in neutrophils is both necessary and sufficient for apoptotic protease-activating factor 1 (Apaf-1)-dependent caspase activation in these cells. PMID: 12615903
  52. function of Apaf-1 is not only to oligomerize procaspase-9 but also to maintain the interaction of the caspase-9 protease domain after processing PMID: 12637514
  53. alternatively spliced APAF-1-ALT is a molecule that is deficient and impeded for mediating apoptosis and may contribute to the resistance to DNA damage-induced treatment observed in LNCaP PMID: 12804598
  54. Bcl-XL directly binds to the ATPase domain of Apaf-1. PMID: 12963020
  55. AMF regulates expression of Apaf-1 and caspase-9 genes via a complex signaling pathway and indirectly regulates formation of the apoptosome. PMID: 14566819
  56. the cytochrome c-Apaf-1-procaspase-9 complex functions in the caspase amplification rather than in its initiation PMID: 14747474
  57. the functional apoptosome complex in apoptotic cells consists primarily of Apaf-1 and processed caspase-9 PMID: 14993223
  58. Loss of APAF-1 expression can be considered an indicator of malignant transformation in melanoma. PMID: 15009102
  59. Allelic imbalance of 12q22-23 associated with APAF-1 locus correlates with poor disease outcome in cutaneous melanoma PMID: 15026369
  60. Apaf-1 proteolytic degradation does not significantly abrogate either the apoptotic morphology or the cleavage of canonical targets. PMID: 15033720
  61. Apaf-1 expression is significantly reduced in human melanoma and Apaf-1 may serve as a therapeutic target in melanoma. PMID: 15305193
  62. results suggest that APAF-1 gene haploinsufficiency caused by AI increases with tumor progression, and relates to hepatic metastasis PMID: 15378005
  63. Limiting Apaf-1 activity may alleviate both pathological protein aggregation and neuronal cell death in HD. PMID: 15590702
  64. there is an inverse correlation between Apaf-1 expression and pathologic stage of melanoma PMID: 15649154
  65. noncytosolic localization of Apaf-1 may constitute a novel mechanism of chemoresistance in B lymphoma PMID: 15692060
  66. Protein kinase A regulates caspase-9 activation by Apaf-1 downstream of cytochrome c PMID: 15703181
  67. Apaf-1 expression in 15 melanoma cell lines PMID: 15832174
  68. Apaf-1 levels vary in melanoma PMID: 15832175
  69. Loss of Apaf-1 expression may represent a marker of aggressive tumor behavior since it correlates significantly with the occurrence of lymph node metastasis in cervical cancer. PMID: 15863166
  70. Methylation silencing is a mechanism of the inactivation of APAF1 in acute leukemia. PMID: 15972851
  71. Using different cell lines of neuronal origin and modulating the expression of both mutant SOD1s and Apaf1, we show that the removal of Apaf1 prevents cells death. PMID: 16046141
  72. No coorelation between apaf-1 expression in melanoma and malignancy PMID: 16098052
  73. The depression-associated alleles thus have a common phenotype that is distinct from that of non-associated variants PMID: 16231040
  74. Apaf-1 gene structure and function and its role in apoptotic machinery. Involvement in melanoma progression and chemoresistance, as well as clinico-pathological relevance of these findings in treatment of this deadly disease. Review. PMID: 16232302
  75. poor prognosis was observed in patients with loss of APAF-1 expression and additional p53 mutation; thus, loss of APAF-1 may become relevant when additional core apoptosis signaling components are disrupted PMID: 16331630
  76. These results suggest that Apaf-1 expression may become a prognostic marker for progress of human cutaneous melanoma and further support the notion that loss of Apaf-1 may be an important contributory factor in the development of the disease. PMID: 16420245
  77. progression of UV-induced apoptosis requires IRES-mediated translation of Apaf-1 to ensure continuous levels of Apaf-1 despite an overall suppression of protein synthesis PMID: 16595687
  78. The promoter hypermethylation of APAF-1 is a marker of aggressive renal cell carcinoma and provides independent prognostic information on disease outcome. PMID: 16951219
  79. Methylation of APAF-1 is associated with bladder and kidney cancer PMID: 17133271
  80. Data support a model in which Apaf-1 is necessary for the cleavage or activation of all procaspases and the promotion of mitochondrial apoptotic events induced by genotoxic drugs. PMID: 17348858
  81. mRNA elevation of apaf1 during blast crisis indicates an involvement in chronic myelogenous leukemia disease progression. PMID: 17361096
  82. APAF-1 methylation is related to transcriptional activity of EZH2 expression in early-stage tumor disease of the bladder. PMID: 17541304
  83. Fas receptor and Apaf-1 were down-regulated in stage III colorectal cancer cell line. PMID: 17603079
  84. The expression of Apaf-1 gene is low in gastric cancer tissues. Methylation of Apaf-1 gene promoter and LOH in domain of 12q22-23 are the main reasons for the expression and altered expression of Apaf-1 gene. PMID: 17876870
  85. Reduced expression of Apaf-1 is associated with colorectal adenocarcinoma PMID: 17882496
  86. These findings suggest that the efflux of K(+) is prerequisite not only for the formation of the apoptosome but also for the downstream apoptotic signal-transduction pathways. PMID: 17885667
  87. These data point to a role for Apaf-1 as a bona fide tumor suppressor. PMID: 18042457
  88. Apaf-1 has a role in regulating cytochrome c induction of apoptosis in brain tumors but not in normal neural tissues PMID: 18093951
  89. Frameshift mutation at mononucleotide repeat in Apaf-1 is rare in gastric carcinomas. PMID: 18373966
  90. PHAPI, CAS, and Hsp70 function together to accelerate nucleotide exchange on Apaf-1 and prevent inactive Apaf-1/cytochrome c aggregation. PMID: 18439902
  91. The methylation profile observed demonstrates a substantial role of the APAF-1 gene in urogenital tumorigenesis. PMID: 18587251
  92. it has been shown that only the isoforms with the extra WD-40 repeat region activate procaspase-9, it suggests that low procaspase-9 activation may also be involved in the deregulation of apoptosis and chemotherapy resistance in acute myeloid leukemia. PMID: 18719738
  93. Frequent HDAC2 mutations are found in MSI tumors and HDAC2 plays a major role in mediating apoptotic response to HDAC inhibitors through direct regulation of APAF1. PMID: 18834886
  94. The oxidative modification of caspase-9 by reactive oxygen species can mediate its interaction with apoptotic protease-activating factor 1, and can promote its formation of disulfide-linked complexes. PMID: 19238172
  95. Data report here the caspase recruitment domain (CARD) of recombinant human apoptotic protease activating factor-1 (Apaf-1) can be induced to undergo amyloid-like fibrillation. PMID: 19603830
  96. analysis of a model of the transition of APAF-1 from inactive monomer to caspase-activating apoptosome PMID: 19801675

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Subcellular Location Cytoplasm
Tissue Specificity Ubiquitous. Highest levels of expression in adult spleen and peripheral blood leukocytes, and in fetal brain, kidney and lung. Isoform 1 is expressed in heart, kidney and liver.
Database Links

HGNC: 576

OMIM: 602233

KEGG: hsa:317

STRING: 9606.ENSP00000448165

UniGene: Hs.552567

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