Product Details

Uniprot No.

Target Names

Alternative Names

APAF 1 antibody; Apaf-1 antibody; APAF_HUMAN antibody; Apaf1 antibody; Apoptotic peptidase activating factor 1 antibody; Apoptotic protease activating factor 1 antibody; Apoptotic protease activating factor antibody; Apoptotic protease-activating factor 1 antibody; CED 4 antibody; CED4 antibody; KIAA0413 antibody

Species Reactivity

Human

Immunogen

A synthesized peptide from human APAF1 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

18D10

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, IHC, FC

Recommended Dilution

Application	Recommended Dilution
IHC	1:50-1:200
FC	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

APAF1 (Apoptotic Protease-Activating Factor 1) serves as a critical molecular scaffold in the intrinsic apoptosis pathway, where it oligomerizes to form the apoptosome complex upon cytochrome c release from mitochondria. This assembly activates caspase-9, initiating the proteolytic cascade that executes programmed cell death. Understanding APAF1 expression and localization provides valuable insights into apoptotic regulation, making it a significant target for researchers investigating cell death mechanisms, cancer biology, and therapeutic resistance.

This recombinant monoclonal antibody, clone 18D10, offers the consistency and reproducibility that demanding experimental workflows require. Generated against a synthetic peptide derived from human APAF1, the antibody is produced using recombinant technology, ensuring sequence-defined specificity and eliminating the lot-to-lot variability often encountered with traditional hybridoma-derived reagents. This reliability proves particularly valuable when establishing long-term studies or comparing results across experiments.

Validation studies demonstrate robust performance across multiple applications. Immunohistochemistry testing in paraffin-embedded human tissues, including skin and cervical cancer samples, shows reliable detection at 1:100 dilution using citrate buffer antigen retrieval. Flow cytometry analysis in HeLa cells confirms intracellular detection capability, with clear signal separation from isotype controls when cells are fixed and permeabilized. These validated applications provide flexibility for researchers examining APAF1 in both tissue architecture contexts and single-cell analyses.

The unconjugated format allows pairing with detection systems of your choice, supporting integration into established laboratory protocols. Whether investigating apoptotic pathway dysregulation in tumor samples or characterizing cell death responses in cultured cells, this antibody delivers the performance consistency essential for meaningful cell biology research.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Code	CSB-RA699080A0HU
Size	US$210
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Image	IHC image of CSB-RA699080A0HU diluted at 1:100 and staining in paraffin-embedded human skin tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. IHC image of CSB-RA699080A0HU diluted at 1:100 and staining in paraffin-embedded human cervical cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Overlay Peak curve showing Hela cells stained with CSB-RA699080A0HU (red line) at 1:100. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100 for 10min. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 35min at 4℃.Control antibody (green line) was Rabbit IgG (1ug/110⁶cells) used under the same conditions. Acquisition of >10, 000 events was performed.
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APAF1 Recombinant Monoclonal Antibody

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