Recombinant Human B-cell linker protein(BLNK)

Code CSB-YP840984HU
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Source Yeast
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Code CSB-EP840984HU
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Source E.coli
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Code CSB-EP840984HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP840984HU
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Source Baculovirus
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Code CSB-MP840984HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names BLNK
Uniprot No. Q8WV28
Alternative Names AGM4; B cell adapter containing SH2 domain protein; B cell adapter containing Src homology 2 domain protein; B cell adaptor containing SH2 domain; B cell linker; B cell linker protein; B cell-specific adaptor protein; B-cell activation; B-cell adapter containing a SH2 domain protein; B-cell adapter containing a Src homology 2 domain protein; B-cell linker protein; BASH; Bca; BLNK; BLNK s; BLNK_HUMAN; Cytoplasmic adapter protein; Ly 57; Ly57; Lymphocyte antigen 57; Lyw 57; Lyw57; MGC111051; SH2 domain-containing leukocyte protein; 65-KD; SLP 65; SLP-65; SLP65; Src homology [SH2] domain-containing leukocyte protein of 65 kD; Src homology 2 domain containing leukocyte protein of 65 kDa; Src homology 2 domain-containing leukocyte protein of 65 kDa
Species Homo sapiens (Human)
Expression Region 1-456
Target Protein Sequence MDKLNKITVP ASQKLRQLQK MVHDIKNNEG GIMNKIKKLK VKAPPSVPRR DYASESPADE EEQWSDDFDS DYENPDEHSD SEMYVMPAEE NADDSYEPPP VEQETRPVHP ALPFARGEYI DNRSSQRHSP PFSKTLPSKP SWPSEKARLT STLPALTALQ KPQVPPKPKG LLEDEADYVV PVEDNDENYI HPTESSSPPP EKAPMVNRST KPNSSTPASP PGTASGRNSG AWETKSPPPA APSPLPRAGK KPTTPLKTTP VASQQNASSV CEEKPIPAER HRGSSHRQEA VQSPVFPPAQ KQIHQKPIPL PRFTEGGNPT VDGPLPSFSS NSTISEQEAG VLCKPWYAGA CDRKSAEEAL HRSNKDGSFL IRKSSGHDSK QPYTLVVFFN KRVYNIPVRF IEATKQYALG RKKNGEEYFG SVAEIIRNHQ HSPLVLIDSQ NNTKDSTRLK YAVKVS
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
(From Uniprot)
Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis.
Gene References into Functions
  1. vesicular signaling scaffolds are required for B cell activation indicates that vesicles may deliver preassembled signaling cargo to sites of BCR activation. PMID: 25140054
  2. early Ca(2+) fluxing provides feed-forward signal amplification by promoting anchoring of the PLCgamma2 C2 domain to phospho-SLP65. PMID: 24166973
  3. up-regulation of BLNK is associated with RUNX1 mutations in cytogenetically normal acute myeloid leukemia. PMID: 22689681
  4. Live cell imaging and co-immunoprecipitation experiments confirmed that both SLP65 and CIN85 are both required for the onset and progression phases of B-cell antigen receptor signal transduction. PMID: 21822214
  5. 16 of the 34 childhood pre-B acute lymphoblastic leukaemia samples that were tested showed a complete loss or drastic reduction of SLP-65 expression PMID: 12761551
  6. The BLNK protein is present in the majority of mediastinal B cell lymphomas. PMID: 15744341
  7. In B cells SLP-65 exists in a 180 kDa complex as well as in monomeric form. PMID: 16356554
  8. V(H) gene rearrangement represents a frequent feature in B-lymphoid malignancy, which can be attributed to SLP65 deficiency in many cases. PMID: 16636677
  9. Syk is required to link phosphorylated SLP-65 to Ca(2+) mobilization. PMID: 17681949
  10. Plasmacytoid dendritic cells express a signalosome consisting of Lyn, Syk, Btk, Slp65 (Blnk) and PLCgamma2. Triggering CD303 leads to tyrosine phosphorylation of Syk, Slp65, PLCgamma2 & cytoskeletal proteins. PMID: 18022864
  11. BLNK prevents aneuploidy by inhibiting cytokinesis. PMID: 19018766
  12. BLNK recruits active H-Ras to the BCR complex, which is essential for sustained surface expression of BCR in the form of the cap and for the signal leading to functional ERK activation PMID: 19218240
  13. Data show that SLP-65 phosphorylation acts upstream for signal initiation and also downstream during selective processing of the B cell receptor signal. PMID: 19372136
  14. Identifies BLNK as a potential target for the protein-tyrosine phosphatase SHP-1. PMID: 15588985

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Involvement in disease Agammaglobulinemia 4, autosomal recessive (AGM4)
Subcellular Location Cytoplasm, Cell membrane
Tissue Specificity Expressed in B-cell lineage and fibroblast cell lines (at protein level). Highest levels of expression in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon.
Database Links

HGNC: 14211

OMIM: 604515

KEGG: hsa:29760

STRING: 9606.ENSP00000224337

UniGene: Hs.665244

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