BLNK Antibody

Datasheet

Code	CSB-PA840984LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Raji whole cell lysate All lanes: BLNK antibody at 2.7µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 51, 49, 45 kDa Observed band size: 51 kDa IHC image of CSB-PA840984LA01HU diluted at 1:800 and staining in paraffin-embedded human spleen tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescent analysis of Hela cells using CSB-PA840984LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) BLNK Polyclonal antibody

Uniprot No.

Q8WV28

Target Names

BLNK

Alternative Names

AGM4 antibody; B cell adapter containing SH2 domain protein antibody; B cell adapter containing Src homology 2 domain protein antibody; B cell adaptor containing SH2 domain antibody; B cell linker antibody; B cell linker protein antibody; B cell-specific adaptor protein antibody; B-cell activation antibody; B-cell adapter containing a SH2 domain protein antibody; B-cell adapter containing a Src homology 2 domain protein antibody; B-cell linker protein antibody; BASH antibody; Bca antibody; BLNK antibody; BLNK s antibody; BLNK_HUMAN antibody; Cytoplasmic adapter protein antibody; Ly 57 antibody; Ly57 antibody; Lymphocyte antigen 57 antibody; Lyw 57 antibody; Lyw57 antibody; MGC111051 antibody; SH2 domain-containing leukocyte protein; 65-KD antibody; SLP 65 antibody; SLP-65 antibody; SLP65 antibody; Src homology [SH2] domain-containing leukocyte protein of 65 kD antibody; Src homology 2 domain containing leukocyte protein of 65 kDa antibody; Src homology 2 domain-containing leukocyte protein of 65 kDa antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human B-cell linker protein (41-337AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The BLNK Antibody (Product code: CSB-PA840984LA01HU) is Non-conjugated. For BLNK Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA840984LB01HU	BLNK Antibody, HRP conjugated	ELISA
FITC	CSB-PA840984LC01HU	BLNK Antibody, FITC conjugated
Biotin	CSB-PA840984LD01HU	BLNK Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Functions as a central linker protein, downstream of the B-cell receptor (BCR), bridging the SYK kinase to a multitude of signaling pathways and regulating biological outcomes of B-cell function and development. Plays a role in the activation of ERK/EPHB2, MAP kinase p38 and JNK. Modulates AP1 activation. Important for the activation of NF-kappa-B and NFAT. Plays an important role in BCR-mediated PLCG1 and PLCG2 activation and Ca(2+) mobilization and is required for trafficking of the BCR to late endosomes. However, does not seem to be required for pre-BCR-mediated activation of MAP kinase and phosphatidyl-inositol 3 (PI3) kinase signaling. May be required for the RAC1-JNK pathway. Plays a critical role in orchestrating the pro-B cell to pre-B cell transition. May play an important role in BCR-induced B-cell apoptosis.

Gene References into Functions

vesicular signaling scaffolds are required for B cell activation indicates that vesicles may deliver preassembled signaling cargo to sites of BCR activation. PMID: 25140054
early Ca(2+) fluxing provides feed-forward signal amplification by promoting anchoring of the PLCgamma2 C2 domain to phospho-SLP65. PMID: 24166973
up-regulation of BLNK is associated with RUNX1 mutations in cytogenetically normal acute myeloid leukemia. PMID: 22689681
Live cell imaging and co-immunoprecipitation experiments confirmed that both SLP65 and CIN85 are both required for the onset and progression phases of B-cell antigen receptor signal transduction. PMID: 21822214
16 of the 34 childhood pre-B acute lymphoblastic leukaemia samples that were tested showed a complete loss or drastic reduction of SLP-65 expression PMID: 12761551
The BLNK protein is present in the majority of mediastinal B cell lymphomas. PMID: 15744341
In B cells SLP-65 exists in a 180 kDa complex as well as in monomeric form. PMID: 16356554
V(H) gene rearrangement represents a frequent feature in B-lymphoid malignancy, which can be attributed to SLP65 deficiency in many cases. PMID: 16636677
Syk is required to link phosphorylated SLP-65 to Ca(2+) mobilization. PMID: 17681949
Plasmacytoid dendritic cells express a signalosome consisting of Lyn, Syk, Btk, Slp65 (Blnk) and PLCgamma2. Triggering CD303 leads to tyrosine phosphorylation of Syk, Slp65, PLCgamma2 & cytoskeletal proteins. PMID: 18022864
BLNK prevents aneuploidy by inhibiting cytokinesis. PMID: 19018766
BLNK recruits active H-Ras to the BCR complex, which is essential for sustained surface expression of BCR in the form of the cap and for the signal leading to functional ERK activation PMID: 19218240
Data show that SLP-65 phosphorylation acts upstream for signal initiation and also downstream during selective processing of the B cell receptor signal. PMID: 19372136
Identifies BLNK as a potential target for the protein-tyrosine phosphatase SHP-1. PMID: 15588985

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Involvement in disease

Agammaglobulinemia 4, autosomal recessive (AGM4)

Subcellular Location

Cytoplasm. Cell membrane. Note=BCR activation results in the translocation to membrane fraction.

Tissue Specificity

Expressed in B-cell lineage and fibroblast cell lines (at protein level). Highest levels of expression in the spleen, with lower levels in the liver, kidney, pancreas, small intestines and colon.

Database Links

HGNC: 14211

OMIM: 604515

KEGG: hsa:29760

STRING: 9606.ENSP00000224337

UniGene: Hs.665244

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Related pathways

NF-kappa B signaling pathway B cell receptor signaling pathway Osteoclast differentiation

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