Recombinant Human Cardiotrophin-like cytokine factor 1 (CLCF1)

Code CSB-YP005479HU
MSDS
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Source Yeast
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Code CSB-EP005479HU
MSDS
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Source E.coli
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Code CSB-EP005479HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP005479HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP005479HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CLCF1
Uniprot No.
Alternative Names
B cell stimulating factor 3; B-cell-stimulating factor 3; BSF 3; BSF-3; BSF3; Cardiotrophin like cytokine; Cardiotrophin like cytokine factor 1; Cardiotrophin-like cytokine factor 1; CISS 2; CISS2; CLC; CLCF 1; Clcf1; CLCF1_HUMAN; Cold induced sweating syndrome 2; CRLF 1 associated cytokine like factor 1; CRLF1 associated cytokine like factor 1; Neurotrophin 1; Neurotrophin 1/B cell stimulating factor 3; Neurotrophin1; NNT 1; NNT-1; Novel neurotrophin 1; Novel neurotrophin-1; NR 6; NR6
Species
Homo sapiens (Human)
Expression Region
28-225
Target Protein Sequence
LNR TGDPGPGPSI QKTYDLTRYL EHQLRSLAGT YLNYLGPPFN EPDFNPPRLG AETLPRATVD LEVWRSLNDK LRLTQNYEAY SHLLCYLRGL NRQAATAELR RSLAHFCTSL QGLLGSIAGV MAALGYPLPQ PLPGTEPTWT PGPAHSDFLQ KMDDFWLLKE LQTWLWRSAK DFNRLKKKMQ PPAAAVTLHL GAHGF
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
In complex with CRLF1, forms a heterodimeric neurotropic cytokine that plays a crucial role during neuronal development (Probable). Also stimulates B-cells. Binds to and activates the ILST/gp130 receptor.
Gene References into Functions
  1. CLF-1, based on its binding site for CLC and on two additional and independent sites for CNTFRalpha and sorLA, is a key player in CLC and CNTFRalpha signaling and turnover. PMID: 26858303
  2. Cardiotrophin-like cytokine factor 1 (CLCF1) and neuropoietin (NP) signalling and their roles in development, adulthood, cancer and degenerative disorders. PMID: 26198769
  3. albuminuria in focal segmental glomerulosclerosis is related to qualitative or quantitative changes in the CLCF1-CRLF1 complex. PMID: 25843671
  4. activation of the Jak-STAT cascade is essential for corticotroph NNT-1/BSF-3 signaling. PMID: 12639901
  5. Structural and docking interaction studies showed that the R197L substitution destabilized the contact site between CLC and CNTFRalpha. PMID: 16782820
  6. Ciliary neurotrophic factor, cardiotrophin-like cytokine, and neuropoietin share a conserved binding site on the ciliary neurotrophic factor receptor alpha chain PMID: 18728012

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Involvement in disease
Cold-induced sweating syndrome 2 (CISS2)
Subcellular Location
Secreted.
Protein Families
IL-6 superfamily
Tissue Specificity
Expressed predominantly in lymph nodes, spleen, peripheral blood lymphocytes, bone marrow, and fetal liver.
Database Links

HGNC: 17412

OMIM: 607672

KEGG: hsa:23529

STRING: 9606.ENSP00000309338

UniGene: Hs.502977

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