Recombinant Human Chromogranin-A(CHGA)

Code CSB-EP005344HU
Size US$1726
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names CHGA
Uniprot No. P10645
Research Area Cancer
Alternative Names Pituitary secretory protein I ;SP-I
Species Homo sapiens (Human)
Source E.coli
Expression Region 19-457aa
Target Protein Sequence LPVNSPMNKGDTEVMKCIVEVISDTLSKPSPMPVSQECFETLRGDERILSILRHQNLLKELQDLALQGAKERAHQQKKHSGFEDELSEVLENQSSQAELKEAVEEPSSKDVMEKREDSKEAEKSGEATDGARPQALPEPMQESKAEGNNQAPGEEEEEEEEATNTHPPASLPSQKYPGPQAEGDSEGLSQGLVDREKGLSAEPGWQAKREEEEEEEEEAEAGEEAVPEEEGPTVVLNPHPSLGYKEIRKGESRSEALAVDGAGKPGAEEAQDPEGKGEQEHSQQKEEEEEMAVVPQGLFRGGKSGELEQEEERLSKEWEDSKRWSKMDQLAKELTAEKRLEGQEEEEDNRDSSMKLSFRARAYGFRGPGPQLRRGWRPSSREDSLEAGLPLQVRGYPEEKKEEEGSANRRPEDQELESLSAIEAELEKVAHQLQALRRG
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 52.9kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA Please contact us to get it.
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Q&A and Customer Reviews

 Q&A
Q:

I have a few questions regarding the CSB-EP005344HU:
1.Previously, we received the information which this product was GST tag fusion protein in 2015 however this was changed and we received a 6x His-tag fusion
Would you happen to know when the tag of this product was changed from GST to 6×His Tag? Do you have a list of products that changed from GST tag to His Tag?
2. It was written "Molecular Weight:52.9 kDa" in the datasheet. We think that this molecular weight is full length human Chromogranin-A. But this product isn't full length human Chromogranin-A and is Expression Data:19-457aa. Can you please clarify?

A:
Thanks for your inquiry. Actually we provide two kinds of tags for this protein. The following are the details of the two kinds of tagged proteins in our newest developed protein catalog.
1. Code: CSB-EP005344HU
Name: Recombinant Human Chromogranin-A(CHGA)
Expression Region: 19-457aa, Full Length
Tag Info: N-terminal 6xHis-tagged
Expression Sequence:

LPVNSPMNKGDTEVMKCIVEVISDTLSKPSPMPVSQECFETLRGDERILSILRHQNLLKELQDLALQGAKERAHQQKKHSGFEDELSEVLENQSSQAELKEAVEEPSSKDVMEKREDSKEAEKSGEATDGARPQALPEPMQESKAEGNNQAPGEEEEEEEEATNTHPPASLPSQKYPGPQAEGDSEGLSQGLVDREKGLSAEPGWQAKREEEEEEEEEAEAGEEAVPEEEGPTVVLNPHPSLGYKEIRKGESRSEALAVDGAGKPGAEEAQDPEGKGEQEHSQQKEEEEEMAVVPQGLFRGGKSGELEQEEERLSKEWEDSKRWSKMDQLAKELTAEKRLEGQEEEEDNRDSSMKLSFRARAYGFRGPGPQLRRGWRPSSREDSLEAGLPLQVRGYPEEKKEEEGSANRRPEDQELESLSAIEAELEKVAHQLQALRRG


Molecular Weight: 52.9 kDa
Note: The region 19-457aa is actually the full length protein, because the signal peptide will be removed for full length of mature protein.
2. Code: CSB-EP005344HU1
Name: Recombinant Human Chromogranin-A(CHGA),partial
Expression Region: 224-457aa, Partial
Tag Info: N-terminal GST-tagged
Expression Sequence:

QAKREEEEEEEEEAEAGEEAVPEEEGPTVVLNPHPSLGYKEIRKGESRSEALAVDGAGKPGAEEAQDPEGKGEQEHSQQKEEEEEMAVVPQGLFRGGKSGELEQEEERLSKEWEDSKRWSKMDQLAKELTAEKRLEGQEEEEDNRDSSMKLSFRARAYGFRGPGPQLRRGWRPSSREDSLEAGLPLQVRGYPEEKKEEEGSANRRPEDQELESLSAIEAELEKVAHQLQALRRG


Molecular Weight: 53.4 kDa
However, from 2015 to 2017, our coding rule has changed several times.
In "CUSABIO Developed Protein Catalog 20170803" (Newest Version), we use CSB-EP005344HU for the N-terminal 6xHis-tagged protein and CSB-EP005344HU1 for the N-terminal GST-tagged protein.
In "CUSABIO Developed Protein Catalog 20170321", we used CSB-EP005344HU for the N-terminal 6xHis-tagged protein and CSB-EP005344HU(1) for the N-terminal GST-tagged protein.
In "CUSABIO Developed Protein Catalog 20160316", we used CSB-EP005344HU for the N-terminal 6xHis-tagged protein and CSB-EP005344HU(G) for the N-terminal GST-tagged protein.
On May 3rd, 2017, you used CSB-EP005344HU to place the order, so we prepare this protein with N-terminal 6xHis-tagged.
For your future orders, could you please list tag type and expression region in your PO?
So we could confirm whether the product information is consistent with our newest version and provide you the correct product you want.
 Customer Reviews

Target Data

Function Pancreastatin
Subcellular Location Cytoplasmic vesicle, secretory vesicle lumen, Cytoplasmic vesicle, secretory vesicle membrane, Secreted, Note=Associated with the secretory granule membrane through direct interaction to SCG3 that in turn binds to cholesterol-enriched lipid rafts in intragranular conditions, SUBCELLULAR LOCATION: Serpinin: Secreted, Cytoplasmic vesicle, secretory vesicle
Protein Families Chromogranin/secretogranin protein family
Tissue Specificity GE-25 is found in the brain.
Database Links

HGNC: 1929

OMIM: 118910

KEGG: hsa:1113

STRING: 9606.ENSP00000216492

UniGene: Hs.150793

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