Recombinant Human Collagen alpha-2 (IV) chain (COL4A2), partial

Code CSB-BP005742HU1
Size $317
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Greater than 85% as determined by SDS-PAGE.
Target Names
Uniprot No.
Research Area
Signal Transduction
Alternative Names
Canstatin; CO4A2_HUMAN; COL4A 2; Col4a2; Collagen alpha 2(IV) chain; Collagen; type IV; alpha 2
Homo sapiens (Human)
Expression Region
Target Protein Sequence
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
48.6 kDa
Protein Length
Tag Info
N-terminal 10xHis-tagged
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
28-38 business days
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.; Canstatin, a cleavage product corresponding to the collagen alpha 2(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity. It inhibits proliferation and migration of endothelial cells, reduces mitochondrial membrane potential, and induces apoptosis. Specifically induces Fas-dependent apoptosis and activates procaspase-8 and -9 activity. Ligand for alphavbeta3 and alphavbeta5 integrins.
Gene References into Functions
  1. Herein, we describe a large family of first-degree relatives affected by a novel heterozygous variant in COL4A2 (c.3490G.A). PMID: 28542708
  2. Data showed that Col4A2 can restrain triple-negative breast cancer (TNBC) cell lines MDA-MB-231 and MDA-MB-468 proliferation, migration, cell cycle, and ultimately lead to apoptosis. The data indicated that Col4A2 was significantly correlated with the proliferation and invasive potential of TNBC. PMID: 27906681
  3. In this study, the inhibitory effect of recombinant canstatin on tumour growth was investigated using a gastric cancer xenograft model. PMID: 27919040
  4. These results of this study provide evidence that the mutation of COL4A2 is associated with lacunar ischemic stroke and deep intracerebral hemorrhage. PMID: 28954878
  5. COL4A2 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
  6. These results indicate that the CHD-related genetic variant at the COL4A2 locus affects COL4A2/COL4A1 expression, smooth muscle cells survival, and atherosclerotic plaque stability, providing a mechanistic explanation for the association between the genetic variant and CHD risk. PMID: 27389912
  7. Expression of miR-26a and miR-29a was significantly down regulated in leukoplakia and cancer tissues but up regulated in lichen planus tissues. Expression of target genes such as, ADAMTS7, ATP1B1, COL4A2, CPEB3, CDK6, DNMT3a and PI3KR1 was significantly down regulated in at least two of three disease types with respect to normal tissues. PMID: 27515006
  8. identified a novel COL4A2 (c.2399G>A, p.G800E, CCDS41907.1) mutation in an autosomal dominant family with porencephaly and ocular abnormalities PMID: 26708157
  9. No significant change in canstatin levels was observed between normotensive and pre-eclampsia patients. PMID: 26343951
  10. Reduction of COL4A2 expression by RNAI-mediated knockdown induces cell death. Finally, elevated Notch3 expression levels correlate with higher COL4A2 expression in human ovarian tumor specimens PMID: 25169943
  11. An association was found between common variation in the COL4A2 gene and sporadic cerebral small vessel disease. PMID: 25653287
  12. Interleukin-1-induced changes in the glioblastoma secretome suggest its role in tumor progression. PMID: 24503185
  13. dominant COL4A2 mutations are a novel major risk factor for familial cerebrovascular disease, including porencephaly and small-vessel disease with reduced penetrance and variable phenotype, which might also be modified by other contributing factors. PMID: 22333902
  14. this study confirmed that abnormalities of the alpha1alpha1alpha2 heterotrimers of type IV collagen cause porencephaly and stresses the importance of screening for COL4A2 as well as for COL4A1. PMID: 22209246
  15. COL4A2 was overexpressed in the ER-negative breast cancer group. PMID: 20805453
  16. an imbalance of glomerular alpha2(IV) and alpha5(IV) chain expression occurred in IgA nephropathy PMID: 12021518
  17. Data show that the NC1 but not 7S domain is essential for chain association and initiation of triple helix formation in human collagen alpha2(IV) . PMID: 15522229
  18. alpha2(IV)NC1 domain has a role in regulating tumor cell behavior PMID: 15743801
  19. Comparison of alpha5(IV) with alpha2(IV) expression in Alport patients is simple and eliminates technical artifacts. PMID: 17554254
  20. support a crucial role for angiogenesis inhibitors in shifting the fate of radiation-induced HIF-1alpha activity from hypoxia-induced tumor radioresistance to hypoxia-induced tumor apoptosis PMID: 17557121
  21. The co-detection of alpha5 and alpha2 chains of collagen IV in frozen skin biopsies is therefore proposed as a simple technique to diagnose Alport syndrome. PMID: 18706356
  22. E1B-55kD-deleted oncolytic adenovirus armed with canstatin gene yields an enhanced anti-tumor efficacy on pancreatic cancer. PMID: 19481338

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Involvement in disease
Porencephaly 2 (POREN2); Intracerebral hemorrhage (ICH)
Subcellular Location
Secreted, extracellular space, extracellular matrix, basement membrane.
Protein Families
Type IV collagen family
Database Links

HGNC: 2203

OMIM: 120090

KEGG: hsa:1284

STRING: 9606.ENSP00000353654

UniGene: Hs.508716

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