Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

COL4A2

Uniprot No.

P08572

Research Area

Cancer

Alternative Names

Canstatin; CO4A2_HUMAN; COL4A 2; Col4a2; Collagen alpha 2(IV) chain; Collagen; type IV; alpha 2

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1493-1712aa

Target Protein Sequence

VKHSQTDQEPMCPVGMNKLWSGYSLLYFEGQEKAHNQDLGLAGSCLARFSTMPFLYCNPGDVCYYASRNDKSYWLSTTAPLPMMPVAEDEIKPYISRCSVCEAPAIAIAVHSQDVSIPHCPAGWRSLWIGYSFLMHTAAGDEGGGQSLVSPGSCLEDFRATPFIECNGGRGTCHYYANKYSFWLTTIPEQSFQGSPSADTLKAGLIRTHISRCQVCMKNL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

28.3kDa

Protein Length

Partial

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Human COL4A2 was expressed with the amino acid range of 1493-1712. This COL4A2 protein is theoretically predicted to have a molecular weight of 28.3 kDa. This COL4A2 protein is produced using e.coli expression system. The COL4A2 gene fragment has been modified by fusing the N-terminal 6xHis tag, providing convenience in detecting and purifying the recombinant COL4A2 protein during the following stages.

The human collagen alpha-2 (IV) chain, encoded by the COL4A2 gene, is a vital component of type IV collagen, a major structural protein found in basement membranes. COL4A2 is essential for maintaining the structural integrity of tissues and organs, especially in the vascular system and basement membranes of various organs. Mutations in COL4A2 can lead to Alport syndrome, a hereditary disorder affecting the kidneys, eyes, and ears. Research on COL4A2 encompasses understanding its role in basement membrane function, tissue development, and its association with various pathological conditions. Investigating COL4A2 provides insights into extracellular matrix biology and has implications for potential therapeutic strategies targeting collagen-related disorders.

Target Background

Function

Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.; Canstatin, a cleavage product corresponding to the collagen alpha 2(IV) NC1 domain, possesses both anti-angiogenic and anti-tumor cell activity. It inhibits proliferation and migration of endothelial cells, reduces mitochondrial membrane potential, and induces apoptosis. Specifically induces Fas-dependent apoptosis and activates procaspase-8 and -9 activity. Ligand for alphavbeta3 and alphavbeta5 integrins.

Gene References into Functions

Herein, we describe a large family of first-degree relatives affected by a novel heterozygous variant in COL4A2 (c.3490G.A). PMID: 28542708
Data showed that Col4A2 can restrain triple-negative breast cancer (TNBC) cell lines MDA-MB-231 and MDA-MB-468 proliferation, migration, cell cycle, and ultimately lead to apoptosis. The data indicated that Col4A2 was significantly correlated with the proliferation and invasive potential of TNBC. PMID: 27906681
In this study, the inhibitory effect of recombinant canstatin on tumour growth was investigated using a gastric cancer xenograft model. PMID: 27919040
These results of this study provide evidence that the mutation of COL4A2 is associated with lacunar ischemic stroke and deep intracerebral hemorrhage. PMID: 28954878
COL4A2 expression is significantly upregulated in human masticatory mucosa during wound healing PMID: 28005267
These results indicate that the CHD-related genetic variant at the COL4A2 locus affects COL4A2/COL4A1 expression, smooth muscle cells survival, and atherosclerotic plaque stability, providing a mechanistic explanation for the association between the genetic variant and CHD risk. PMID: 27389912
Expression of miR-26a and miR-29a was significantly down regulated in leukoplakia and cancer tissues but up regulated in lichen planus tissues. Expression of target genes such as, ADAMTS7, ATP1B1, COL4A2, CPEB3, CDK6, DNMT3a and PI3KR1 was significantly down regulated in at least two of three disease types with respect to normal tissues. PMID: 27515006
identified a novel COL4A2 (c.2399G>A, p.G800E, CCDS41907.1) mutation in an autosomal dominant family with porencephaly and ocular abnormalities PMID: 26708157
No significant change in canstatin levels was observed between normotensive and pre-eclampsia patients. PMID: 26343951
Reduction of COL4A2 expression by RNAI-mediated knockdown induces cell death. Finally, elevated Notch3 expression levels correlate with higher COL4A2 expression in human ovarian tumor specimens PMID: 25169943
An association was found between common variation in the COL4A2 gene and sporadic cerebral small vessel disease. PMID: 25653287
Interleukin-1-induced changes in the glioblastoma secretome suggest its role in tumor progression. PMID: 24503185
dominant COL4A2 mutations are a novel major risk factor for familial cerebrovascular disease, including porencephaly and small-vessel disease with reduced penetrance and variable phenotype, which might also be modified by other contributing factors. PMID: 22333902
this study confirmed that abnormalities of the alpha1alpha1alpha2 heterotrimers of type IV collagen cause porencephaly and stresses the importance of screening for COL4A2 as well as for COL4A1. PMID: 22209246
COL4A2 was overexpressed in the ER-negative breast cancer group. PMID: 20805453
an imbalance of glomerular alpha2(IV) and alpha5(IV) chain expression occurred in IgA nephropathy PMID: 12021518
Data show that the NC1 but not 7S domain is essential for chain association and initiation of triple helix formation in human collagen alpha2(IV) . PMID: 15522229
alpha2(IV)NC1 domain has a role in regulating tumor cell behavior PMID: 15743801
Comparison of alpha5(IV) with alpha2(IV) expression in Alport patients is simple and eliminates technical artifacts. PMID: 17554254
support a crucial role for angiogenesis inhibitors in shifting the fate of radiation-induced HIF-1alpha activity from hypoxia-induced tumor radioresistance to hypoxia-induced tumor apoptosis PMID: 17557121
The co-detection of alpha5 and alpha2 chains of collagen IV in frozen skin biopsies is therefore proposed as a simple technique to diagnose Alport syndrome. PMID: 18706356
E1B-55kD-deleted oncolytic adenovirus armed with canstatin gene yields an enhanced anti-tumor efficacy on pancreatic cancer. PMID: 19481338

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Involvement in disease

Porencephaly 2 (POREN2); Intracerebral hemorrhage (ICH)

Subcellular Location

Secreted, extracellular space, extracellular matrix, basement membrane.

Protein Families

Type IV collagen family

Database Links

HGNC: 2203

OMIM: 120090

KEGG: hsa:1284

STRING: 9606.ENSP00000353654

UniGene: Hs.508716

Code	CSB-EP005742HU
Abbreviation	Recombinant Human COL4A2 protein, partial
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Collagen alpha-2 (IV) chain (COL4A2), partial

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