Recombinant Human Collagen alpha-6 (IV) chain (COL4A6), partial

Code CSB-YP619857HU
MSDS
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Source Yeast
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Code CSB-EP619857HU
MSDS
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Source E.coli
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Code CSB-EP619857HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP619857HU
MSDS
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Source Baculovirus
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Code CSB-MP619857HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
COL4A6
Uniprot No.
Alternative Names
COL4A6Collagen alpha-6(IV) chain
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Type IV collagen is the major structural component of glomerular basement membranes (GBM), forming a 'chicken-wire' meshwork together with laminins, proteoglycans and entactin/nidogen.
Gene References into Functions
  1. Mutation in COL4A6 gene is associated with gastric cancer peritoneal carcinomatosis. PMID: 26811494
  2. COL4A6 is associated with X-linked nonsyndromic hearing loss. PMID: 23714752
  3. We showed that TPM2, CLU, and COL4A6 mRNA levels are downregulated in prostate cancer. PMID: 23621580
  4. expression of collagen type IV alpha6 chain in the smooth muscle BM of the gastrointestinal tract is restricted to the esophagus in humans PMID: 20951201
  5. The expression of collagen type IV was significantly decreased in colorectal cancer tissues. PMID: 18782525
  6. Enhanced expression of collagen type IV is associated with liver metastases from gastrointestinal tumours PMID: 15679046
  7. This study showed that in some cases of esophageal squamous cell carcinoma the alpha5/alpha6 (IV) chains are expressed linearly around the cancer cell nests and the alpha5/alpha6 (IV) chain expression is one prognostic factor of ESCC. PMID: 17955302
  8. Type IV collagen alpha6 chain-derived noncollagenous domain 1 (alpha6NC1) is an endogenous inhibitor of angiogenesis and tumor growth. PMID: 18074349
  9. Meningiomas increase in size through increased production of extracellular matrix; furthermore, the proliferation of cells typically associated with neoplasia requires considerable interaction with the extracellular matrix. PMID: 18474427

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Involvement in disease
Deafness, X-linked, 6 (DFNX6)
Subcellular Location
Secreted, extracellular space, extracellular matrix, basement membrane.
Protein Families
Type IV collagen family
Database Links

HGNC: 2208

OMIM: 300914

KEGG: hsa:1288

STRING: 9606.ENSP00000361290

UniGene: Hs.145586

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