Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

MMP13

Uniprot No.

P45452

Research Area

Developmental Biology

Alternative Names

CLG 3; CLG3; Collagenase 3; Collagenase3; MANDP1; Matrix metallopeptidase 13 (collagenase 3); Matrix Metalloproteinase 13; Matrix metalloproteinase-13; MMP 13; MMP-13; Mmp13; MMP13_HUMAN

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

104-471aa

Target Protein Sequence

YNVFPRTLKWSKMNLTYRIVNYTPDMTHSEVEKAFKKAFKVWSDVTPLNFTRLHDGIADIMISFGIKEHGDFYPFDGPSGLLAHAFPPGPNYGGDAHFDDDETWTSSSKGYNLFLVAAHEFGHSLGLDHSKDPGALMFPIYTYTGKSHFMLPDDDVQGIQSLYGPGDEDPNPKHPKTPDKCDPSLSLDAITSLRGETMIFKDRFFWRLHPQQVDAELFLTKSFWPELPNRIDAAYEHPSHDLIFIFRGRKFWALNGYDILEGYPKKISELGLPKEVKKISAAVHFEDTGKTLLFSGNQVWRYDDTNHIMDKDYPRLIEEDFPGIGDKVDAVYEKNGYIYFFNGPIQFEYSIWSNRIVRVMPANSILWC
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

58.3kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Amino acids 104-471 form the expressed segment for recombinant Human MMP13. The calculated molecular weight for this MMP13 protein is 58.3 kDa. This protein is generated in a e.coli-based system. The MMP13 gene fragment has been modified by fusing the N-terminal 6xHis-SUMO tag, providing convenience in detecting and purifying the recombinant MMP13 protein during the following stages.

Collagenase 3, also known as matrix metalloproteinase 13 (MMP13), is a zinc-dependent endopeptidase involved in the degradation of extracellular matrix components, particularly type II collagen in cartilage. MMP13 plays a crucial role in tissue remodeling, wound healing, and skeletal development. Its dysregulation is associated with various pathological conditions, including osteoarthritis and rheumatoid arthritis. Research on MMP13 spans multiple areas, including musculoskeletal biology, connective tissue disorders, and inflammatory diseases. Investigating the regulatory mechanisms and signaling pathways involving MMP13 can provide insights into therapeutic strategies for conditions involving abnormal tissue remodeling and degradation of the extracellular matrix.

Target Background

Function

Plays a role in the degradation of extracellular matrix proteins including fibrillar collagen, fibronectin, TNC and ACAN. Cleaves triple helical collagens, including type I, type II and type III collagen, but has the highest activity with soluble type II collagen. Can also degrade collagen type IV, type XIV and type X. May also function by activating or degrading key regulatory proteins, such as TGFB1 and CCN2. Plays a role in wound healing, tissue remodeling, cartilage degradation, bone development, bone mineralization and ossification. Required for normal embryonic bone development and ossification. Plays a role in the healing of bone fractures via endochondral ossification. Plays a role in wound healing, probably by a mechanism that involves proteolytic activation of TGFB1 and degradation of CCN2. Plays a role in keratinocyte migration during wound healing. May play a role in cell migration and in tumor cell invasion.

Gene References into Functions

MMP-13 plays a critical role in the shedding/cleavage of PD-L1 in the human head and neck squamous cell carcinoma. PMID: 29345283
Data suggest that matrix metalloproteinase 13 (MMP-13) and its regulatory networks are suitable targets for the development of effective early treatment strategies for osteoarthritis (OA). PMID: 29126436
High Expressions of MMP-13 is associated with degenerative knee osteoarthritis. PMID: 28418842
Transfection of cells with either miR-100 or miR-125b negated the induction of MMP13 release. Additionally, AR activation induced a morphological alteration of MDA-MB-453 cells, which was blocked by miR-125b only. PMID: 28816390
ATM could be activated by lung cancer-associated TNF-alpha, up-regulate MMP-13 expression and thereby augment tumor metastasis PMID: 27556690
The Sp1-mediaded allelic regulation of MMP13 expression by an esophageal squamous cell carcinoma susceptibility SNP rs2252070 has been demonstrated. PMID: 27245877
MMP-13 IRS represents a suitable method to assess pathologic grade of precancerous and cancerous colorectal lesions. MMP-13 has been identified as an excellent marker of high grade IEN and CRC, and may thus be applied for prognostic stratification. PMID: 27716617
These findings suggest that using MMP-13 inhibitors in diffuse group might contribute to the control of tumor growth PMID: 28128735
A strong association between the -77 MMP-13 polymorphism and posterior tibial tendinopathy insufficiency. PMID: 27886420
TGF-beta1 stimulates the phosphorylation of Runx2 at three serine amino acids, and this event is required for MMP-13 expression in osteoblastic cells. PMID: 28419442
IL-6-stimulated MMP-13 expression was independent of IL-1beta stimulation and was blocked by SAHA, suggesting that SAHA inhibits IL-6 signaling in Osteoarthritis (OA) chondrocytes. PMID: 27555113
High MMP13 expression is associated with intervertebral disc degeneration. PMID: 28559201
data demonstrate that MMP-13 is critical for the development of osteolytic lesions in Multiple myeloma and that targeting the MMP-13 protein - rather than its catalytic activity - constitutes a potential approach to mitigating bone disease in affected patients. PMID: 27043283
Endoplasmic reticulum stress participates in the progress of senescence and apoptosis of osteoarthritic chondrocytes, which manifested in increased expression of ADAMTS5, MMP13, and decreased COL2A1 expression. PMID: 28728848
these findings support roles for both cFOS (indirect) and ATF3 (direct) in effecting MMP13 transcription in human chondrocytes. PMID: 27956552
In osteoarthritis (OA) chondrocytes, hydrostatic pressure (HP) restores the expression levels of some miRNAs, downregulates MMP-13, ADAMTS-5, and HDAC-4, and modulates the Wnt/beta-catenin pathway activation. PMID: 28085114
These results suggested that HIF-2alpha may cause meniscal matrix degradation by transactivation of MMP-13 PMID: 26892680
TGF-beta1 stimulated a sustained and prolonged expression of ATF-3, and its interaction and regulation of c-Jun protein and their assembly as a protein complex at the AP-1 site of the MMP-13 promoter could be responsible for MMP-13 gene activation in MDA-MB231 cells. PMID: 27751807
Serum MMP13 did not differ significantly between normal weight, overweight, and obese participants. PMID: 27296149
No significant association was observed in MMP13, TIMP2 and TGFB3 genes with CP or PI. CP is a risk factor to develop PI, however, there is no association of both diseases with polymorphisms in the MMP13, TIMP2 and TGFB3 genes PMID: 27058373
Findings suggest thatSirt1 may regulate the expression of Runx2, which is the osteogenic transcription factor, and the production of MMP-13 from chondrocytes in OA. PMID: 27367673
the mechanisms and roles of MMP-13 secretion in human small airway epithelial cells and functional polymorphisms of the MMP13 gene PMID: 26635116
as the grade of late-stage osteoarthritis advanced, levels of TNF-alpha in the serum became elevated, whereas levels of MMP-13 in the synovial fluid became elevated PMID: 27130394
As the grade of late stage osteoarthritis advances, increase in synovial MMP-13 and serum TNF-a levels become pronounced. PMID: 27932045
High MMP13 serum levels are associated with Esophageal Squamous Cell Carcinoma. PMID: 27356690
Our study demonstrates that miR-148a inhibits the migration of breast cancer cells by targeting MMP-13 PMID: 26298724
MMP13 expression positively related to the lymph node and distal metastasis, tumor stage and relapse of colorectal cancer PMID: 25726157
in degenerative intervertebral discs, IL1b upregulates NFkB, MMP13 and ADAMTS4 PMID: 25433723
Expression of MMP13 negatively correlated with miR-27b expression in degenerative nucleus pulposus tissues. PMID: 26583473
sustained released resveratrol inhibits interleukin-1beta-induced metalloproteinase-13 activation and promotes chondrocyte differentiation PMID: 26526931
Report modified platelet deposition on matrix metalloproteinase 13 digested collagen I PMID: 26447617
GP73 enhances MMP-13 expression through cAMP responsive element binding protein (CREB)-mediated transcription activation. Levels of GP73 and MMP-13 are increased and positively correlated in human HCC tissues. Augmented MMP-13 potentiates HCC cell metastasis. PMID: 26378022
SENP2 inhibits MMP13 expression in BC cells through de-SUMOylation of TBL1/TBLR1, which inhibits nuclear translocation of beta-catenin. PMID: 26369384
Hyaluronic acid exhibits a pronounced suppressive effect on MMP-13 expression in osteoarthritis. PMID: 26934732
Elevated levels of MMP-13 may play a role in the pathogenesis of chronic periodontitis. There is a direct correlation of increased expression of MMP-13 with various clinical and histologic parameters in disease severity PMID: 25006778
IL-1Ra is associated with MMP-13 expression and has a novel function in such regulation without interference of the IL-1 signaling cascade. PMID: 26474296
siRNA inhibited the effect of MMP13-containing exosomes on tumor cells metastasis as well as angiogenesis PMID: 26362844
RKIP Inhibits Local Breast Cancer Invasion by Antagonizing the Transcriptional Activation of MMP13 PMID: 26308852
together, these data demonstrate that SERPINE2 might prevent cartilage catabolism by inhibiting the expression of MMP-13, one of the most relevant collagenases, involved in cartilage breakdown in OA. PMID: 26305372
High expression of matrix metalloproteinase-13 is associated with pancreatic cancer. PMID: 25948792
Together, these data suggest that shikonin may suppress Osteosarcoma invasiveness through MMP13 suppression. Thus, our data highlight a previous unappreciated role for shikonin in suppressing Osteosarcoma cell metastasis PMID: 26104765
our results revealed that activation of the P2X7 receptor by ATP promotes breast cancer cell invasion and migration, possibly via activation of the AKT pathway and regulation of E-cadherin and MMP-13 expression. PMID: 25976617
MMP-13 was associated with metastasis and poor survival in 79 patients with melanoma. MMP-13 expression was inversely correlated with vasculogenic mimicry. MMP-13 cleaves laminin-5 gamma 2 to accelerate metastasis. PMID: 25749207
Together, these data suggest that GRh2 may suppress GBM migration through inhibiting Akt-mediated MMP13 activation. PMID: 25835975
Both VEGF-C and MMP13 are significantly upregulated in Multiple Myeloma with lymph-node metastases. PMID: 25966777
IL-32 stimulation promotes the invasion and motility of osteosarcoma cells, possibly via the activation of AKT and the upregulation of MMP-13 expression. PMID: 25846944
Up-regulation of matrix metalloproteinase 13 is associated with colitis-associated cancer. PMID: 25742789
Data show that knockdown of endogenous periostin attenuates constitutive matrix metalloproteinase-13 (MMP-13) expression. PMID: 26092928
Clusterin is an independent predictive factor for prognosis of hepatocellular carcinoma and it facilitates metastasis through EIF3I/Akt/MMP13 signaling. PMID: 25609201
High level of protein expression of MMP13 was significantly associated with poor prognosis in oral squamous cell carcinoma. PMID: 25401159

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Involvement in disease

Spondyloepimetaphyseal dysplasia Missouri type (SEMD-MO); Metaphyseal anadysplasia 1 (MANDP1); Metaphyseal dysplasia, Spahr type (MDST)

Subcellular Location

Secreted, extracellular space, extracellular matrix. Secreted.

Protein Families

Peptidase M10A family

Tissue Specificity

Detected in fetal cartilage and calvaria, in chondrocytes of hypertrophic cartilage in vertebrae and in the dorsal end of ribs undergoing ossification, as well as in osteoblasts and periosteal cells below the inner periosteal region of ossified ribs. Dete

Database Links

HGNC: 7159

OMIM: 250400

KEGG: hsa:4322

STRING: 9606.ENSP00000260302

UniGene: Hs.2936

Code	CSB-EP014660HU
Abbreviation	Recombinant Human MMP13 protein
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Human Collagenase 3 (MMP13)

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