Recombinant Human Docking protein 3 (DOK3)

Code CSB-YP007109HU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP007109HU
MSDS
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Source E.coli
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Code CSB-EP007109HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007109HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP007109HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Uniprot No.
Alternative Names
Docking protein 3; DOK 3; DOK Like Protein; Dok3; DOK3_HUMAN; DOKL Pending; Downstream of tyrosine kinase 3; p62 DOK Like Protein
Species
Homo sapiens (Human)
Expression Region
1-496
Target Protein Sequence
MTRGARLRSD ARAQLNQLSL DGGTGSGQKG KCEEFPSSLS SVSPGLEAAA LLLAVTMDPL ETPIKDGILY QQHVKFGKKC WRKVWALLYA GGPSGVARLE SWEVRDGGLG AAGDRSAGPG RRGERRVIRL ADCVSVLPAD GESCPRDTGA FLLTTTERSH LLAAQHRQAW MGPICQLAFP GTGEASSGST DAQSPKRGLV PMEENSIYSS WQEVGEFPVV VQRTEAATRC QLKGPALLVL GPDAIQLREA KGTQALYSWP YHFLRKFGSD KGVFSFEAGR RCHSGEGLFA FSTPCAPDLC RAVAGAIARQ RERLPELTRP QPCPLPRATS LPSLDTPGEL REMPPGPEPP TSRKMHLAEP GPQSLPLLLG PEPNDLASGL YASVCKRASG PPGNEHLYEN LCVLEASPTL HGGEPEPHEG PGSRSPTTSP IYHNGQDLSW PGPANDSTLE AQYRRLLELD QVEGTGRPDP QAGFKAKLVT LLSRERRKGP APCDRP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
DOK proteins are enzymatically inert adaptor or scaffolding proteins. They provide a docking platform for the assembly of multimolecular signaling complexes. DOK3 is a negative regulator of JNK signaling in B-cells through interaction with INPP5D/SHIP1. May modulate ABL1 function.
Gene References into Functions
  1. Mutations in DOK3 gene is associated with prostate cancer PMID: 26585945
  2. DOK2 and DOK3 expression was significantly reduced in HTLV-1-infected T cells. PMID: 27265473
  3. The Dok-3/Grb2 protein signal module attenuates Lyn kinase-dependent activation of Syk kinase in B cell antigen receptor microclusters PMID: 23223229
  4. absence of DOK3 increases LPS signaling, contributing to LPS-induced tolerance. Thus, DOK3 plays a role in TLR signaling during both naive and endotoxin-induced tolerant conditions PMID: 22761938
  5. Identification of DOK genes as lung tumor suppressors. PMID: 20139980
  6. The novel platelet adapter Dok-3 is tyrosine phosphorylated in an Src kinase-independent manner downstream of alphaIIbbeta3 in human platelets, leading to an interaction with Grb2 and SHIP-1. PMID: 19682241
  7. findings indicate that Dok-3 sequesters Grb2 from Shc and inhibits the Ras-Erk pathway downstream of PTKs PMID: 16436051

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Subcellular Location
Cytoplasm. Cell membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families
DOK family, Type A subfamily
Tissue Specificity
Expressed in spleen.
Database Links

HGNC: 24583

OMIM: 611435

KEGG: hsa:79930

STRING: 9606.ENSP00000349727

UniGene: Hs.720849

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