Recombinant Human Elafin(PI3)

Code CSB-YP017952HU
Size US$1298
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP017952HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) PI3.
  • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP017952HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) PI3.
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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names PI3
Uniprot No. P19957
Research Area Signal Transduction
Alternative Names Cementoin; ELAF_HUMAN; Elafin; Elafin/Skalp; Elastase Specific Inhibitor; Elastase-specific inhibitor; ES 1; ESI; Peptidase inhibitor 3; Peptidase inhibitor 3; skin derived; PI 3; PI-3; PI3; Pre elafin; Protease inhibitor 3 skin derived ; Protease inhibitor 3; skin derived (SKALP); Protease inhibitor WAP3; SKALP; Skin derived Anti leukoproteinase; Skin-derived antileukoproteinase; Trappin 2; WAP four disulfide core domain 14; WAP four disulfide core domain protein 14; WAP four-disulfide core domain protein 14; WAP3; WFDC14
Species Homo sapiens (Human)
Source Yeast
Expression Region 61-117aa
Target Protein Sequence AQEPVKGPVSTKPGSCPIILIRCAMLNPPNRCLKDTDCPGIKKCCEGSCGMACFVPQ
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 8.0kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis.
Gene References into Functions
  1. Elafin decreased in active patients with inflammatory bowel disease and was negatively correlated with disease activity, suggesting that elafin may play a protective role PMID: 29084078
  2. Plasma Neutrophil Elastase and Elafin as Prognostic Biomarker for Acute Respiratory Distress Syndrome PMID: 28187039
  3. UV phototoxicity-induced pre-elafin inside keratinocytes prior to cornified envelope formation could be involved in UV-induced keratinocyte apoptosis via cystatin-A downregulation resulting in pro-caspase-3 activation. PMID: 28119996
  4. Recombinant human elafin promotes alveologenesis in newborn mice exposed to chronic hyperoxia PMID: 28802561
  5. significantly higher expression in preterm prelabor ruptured fetal membranes than in membranes from term birth PMID: 27580179
  6. Our study revealed that high elafin levels identified in smoking- and asthma-related microarray data sets and an epidemiologic study significantly reduced the risk of asthma. PMID: 26932165
  7. The expression of elafin in fallopian tubes of ectopic pregnancies is reduced. PMID: 25356943
  8. The elevations in cervical elafin and SLPI expression in the women with preterm delivery might reflect the local response to the pathogen invasion into the cervix preceding preterm labor. PMID: 25559229
  9. PI3 expression was downregulated in 24% of DCIS and 83% of invasive breast tumors. PI3 was also downregulated in 33% of ovarian cystadenomas, 43% of borderline ovarian tumors, and 86% of invasive ovarian carcinomas. PMID: 25551582
  10. The imbalance between neutrophil elastase and its inhibitors, such as elafin, presents an important therapeutic target in breast cancer. PMID: 25195861
  11. Higher urinary elafin levels are associated with an increased risk of micro- and macroalbuminuria, acute kidney injury and chronic kidney disease, and death after hematopoietic stem cell transplantation. PMID: 25388519
  12. cutaneous elafin does not seem to discriminate acute graft-versus-host disease from drug hypersensitivity rash lesions, but there may be an association between cutaneous elafin expression and poor prognosis for patients with cutaneous GVHD PMID: 25405322
  13. Data indicate that elafin overexpression is associated with poor overall survival. PMID: 24469047
  14. Low expression of elafin gene correlated with significantly reduced time to relapse, and when combined with high expression of elastase gene was associated with decreased survival in breast cancer patients PMID: 23320734
  15. Elafin has important roles in normal homoeostasis and at sites of inflammation. These roles include antiprotease and antimicrobial activity. PMID: 19906197
  16. Results confirm the association between SNP rs2664581 and enhanced risk of developing acute respiratory distress syndrome. PMID: 24617927
  17. Tubal epithelium from women with hydrosalpinx has lower levels of elafin expression. PMID: 23885101
  18. Elafin expression in the small intestinal epithelium was lower in patients with active celiac disease compared with control patients. In vitro, elafin significantly slowed the kinetics of the deamidation of the 33-mer peptide to its more immunogenic form. PMID: 24710505
  19. Production of HBD2 and elafin by vaginal epithelial cells is sensitive to E, suggesting that innate immune protection varies in the vagina across the menstrual cycle. PMID: 23398087
  20. Elafin expression may establish the diagnosis of pre-engraftment syndrome and graft versus host disease. PMID: 23866880
  21. Trappin-2 and elafin significantly reduce HSV-2 viral attachment to, and replication in genital epithelial cells. PMID: 23637403
  22. The N-terminus of elafin is critical for intranuclear localization and anti-HIV-1 activity. PMID: 23300756
  23. Studies indicate that Trappin-2/Elafin possessing anti-microbial properties against different classes of pathogens including viruses, fungi and bacteria. PMID: 22634606
  24. The results suggest that decreased expression of elastin and LOX and increased expression of elafin in the cardinal ligaments may contribute to pelvic organ prolapse. PMID: 19087518
  25. The endometrium of women with hydrosalpinx has an increased number of neutrophils and lower expression of elafin, an elastase inhibitor and natural antimicrobial molecule. PMID: 21392745
  26. these results collectively suggest that, in melanoma cells, Foxa2 expression is silenced and therefore elafin is maintained unexpressed to facilitate cell proliferation in the disease melanoma. PMID: 21466784
  27. ability to modulate the expression of some P.aeruginosa virulence factors PMID: 20932308
  28. These results suggest that elafin can maintain airway epithelium integrity by protecting airway epithelial cells and enhancing the anti-inflammatory capability of airway. PMID: 19823954
  29. increased production at the secretory phase of the menstrual cycle relative to the proliferative phase PMID: 19824918
  30. Elafin has a role in graft-versus-host disease of the skin PMID: 20371463
  31. Elafin may serve as a determinant of poor survival in serous ovarian carcinomas PMID: 20126474
  32. Overexpression of elafin in epithelial cells attenuated the damage of Pseudomonas aeruginosa biofilm. PMID: 19420899
  33. Elafin is a component of cervicovaginal secretions in pregnancy, and levels are diminished in bacterial vaginosis. PMID: 19723838
  34. Synthesized locally at mucosal sites. Efficiently inhibit target enzymes released into interstitium. Might be important in controlling excessive neutrophil elastase release in chronic obstructive pulmonary disease and cystic fibrosis. (review) PMID: 11667971
  35. regulation of the elafin gene is dependent on chromatin structure in keratinocytes PMID: 12542536
  36. results indicate that elafin may have a dual function, promoting up-regulation of local lung innate immunity while simultaneously down-regulating potentially unwanted systemic inflammatory responses in the circulation PMID: 12819058
  37. elafin regulates proteolytic enzymes during menstruation and contributes to the innate defense against uterine infection PMID: 12970320
  38. Serine elastase inhibition (elafin overexpression) appears to suppress inflammation, cardiac dilatation, and dysfunction after myocardial infarct. PMID: 14693682
  39. Gene augmentation of human elafin suggests a novel and extended anti-inflammatory role for this human neutrophil elastase inhibitor working as an effector of innate immunity to protect tissues against maladaptive inflammatory responses. PMID: 15034071
  40. The differential biological activity of elafin in different conditions suggests a role for this molecule in either LPS detoxification or activation of innate immune responses, depending on the external cellular environment. PMID: 15668324
  41. Oxidized elafin poorly inhibits the elastolytic activity of leukocyte elastase and proteinase 3. PMID: 16279952
  42. transglutaminase 2-mediated cross-linking of serine protease inhibitors elafin and trappin-2 to neutrophil serine proteinases preserves their inhibitory capacities PMID: 16300411
  43. The pleiotropic molecule elafin has significant potential in modulating antigen-presenting cell numbers and activity, and could be beneficial in mucosal protective strategies. PMID: 16424380
  44. The promoter sequences of PI3 have a high degree of variability. PMID: 16719916
  45. beneficial effects of pre-elafin could be mediated, at least in part, by its ability to increase G-CSF levels in the lung PMID: 16913840
  46. The transglutaminase substrate moiety of elafin plays an important role in anchoring elafin at its proper sites of action during UV-induced aging processes. PMID: 17139263
  47. Levels of mRNA and immunostaining of the antiproteases elafin and SLPI were enhanced strongly in inflamed versus noninflamed UC. Elafin and SLPI may be added to the list of defensin-like peptides with diminished induction in CD versus UC. PMID: 17200145
  48. full antipeptidase activity of pre-elafin is essential to protect against lung tissue lesions PMID: 17489739
  49. This review focuses on recent findings revealing that elafin has many biological functions as diverse as anti-bacterial, anti-fungal, anti-viral, anti-inflammatory and immuno-modulatory functions. PMID: 17964057
  50. We report here that pre-elafin inhibits a secreted peptidase to prevent Pseudomonas aeruginosa growth in vitro. PMID: 18025118
  51. High levels of PI3 is associated with glioblastoma PMID: 18092325
  52. Results of a genome-wide expression analysis in blood identify pre-elafin as a biomarker in acute respiratory distress syndrome. PMID: 18203972
  53. elafin is cleaved by its cognate enzyme NE present at excessive concentration in CF sputum, an effect which is promoted by P. aeruginosa infection PMID: 18799464
  54. SLPI and elafin have a role in the innate immune defence of the Fallopian tube in infection and ectopic pregnancy PMID: 19095674
  55. The hypothesis that an imbalance between neutrophil elastase (HNE) and its inhibitors in blood is related to the development of ARDS, was tested. PMID: 19197381
  56. polymorphisms in PI3 gene are significantly associated with ARDS risk and with circulating PI3 levels PMID: 19251943
  57. The synthesis of elafin is a novel effector function of gammadelta T cells which might be important for local immune defence PMID: 19906197
  58. Genetically augmented elafin has the capacity to protect the lung in vivo and in vitro against the injurious effects of antibiotic-resistant bacterial pathogens and whole activated neutrophils. PMID: 11466403

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Subcellular Location Secreted
Database Links

HGNC: 8947

OMIM: 182257

KEGG: hsa:5266

STRING: 9606.ENSP00000243924

UniGene: Hs.112341

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