Recombinant Human Elafin (PI3)

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Code	CSB-EP017952HU
Abbreviation	Recombinant Human PI3 protein
MSDS	MSDS Datasheet
Size	$224
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details
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Target Background

Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

PI3

Uniprot No.

P19957

Research Area

Signal Transduction

Alternative Names

Cementoin; ELAF_HUMAN; Elafin; Elafin/Skalp; Elastase Specific Inhibitor; Elastase-specific inhibitor; ES 1; ESI; Peptidase inhibitor 3; Peptidase inhibitor 3; skin derived; PI 3; PI-3; PI3; Pre elafin; Protease inhibitor 3 skin derived ; Protease inhibitor 3; skin derived (SKALP); Protease inhibitor WAP3; SKALP; Skin derived Anti leukoproteinase; Skin-derived antileukoproteinase; Trappin 2; WAP four disulfide core domain 14; WAP four disulfide core domain protein 14; WAP four-disulfide core domain protein 14; WAP3; WFDC14

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

61-117aa

Target Protein Sequence

AQEPVKGPVSTKPGSCPIILIRCAMLNPPNRCLKDTDCPGIKKCCEGSCGMACFVPQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

22.0kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

CUSABIO team inserts the gene coding for the Human PI3 protein (61-117aa) into a plasmid vector to form recombinant plasmid, which is then introduced into e.coli cells. e.coli cells demonstrating successful uptake of the recombinant plasmid are selected based on their ability to survive in the presence of a specific antibiotic. The positive e.coli cells are cultured under conditions that promote the expression of the gene of interest. A N-terminal 6xHis-SUMO tag is linked to the protein. Following expression, affinity purification is used to isolate and purify the recombinant Human PI3 protein from the cell lysate. Denaturing SDS-PAGE is then applied to resolve the resulting recombinant Human PI3 protein, demonstrating a purity greater than 90%.

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Target Background

Function

Neutrophil and pancreatic elastase-specific inhibitor of skin. It may prevent elastase-mediated tissue proteolysis. Has been shown to inhibit the alpha-4-beta-2/CHRNA2-CHRNB2 nicotinic acetylcholine receptor and to produce a weak inhibition on Kv11.1/KCNH2/ERG1 and on the transient receptor potential cation channel subfamily V member 1 (TRPV1).

Gene References into Functions

Elafin decreased in active patients with inflammatory bowel disease and was negatively correlated with disease activity, suggesting that elafin may play a protective role PMID: 29084078
Plasma Neutrophil Elastase and Elafin as Prognostic Biomarker for Acute Respiratory Distress Syndrome PMID: 28187039
UV phototoxicity-induced pre-elafin inside keratinocytes prior to cornified envelope formation could be involved in UV-induced keratinocyte apoptosis via cystatin-A downregulation resulting in pro-caspase-3 activation. PMID: 28119996
Recombinant human elafin promotes alveologenesis in newborn mice exposed to chronic hyperoxia PMID: 28802561
significantly higher expression in preterm prelabor ruptured fetal membranes than in membranes from term birth PMID: 27580179
Our study revealed that high elafin levels identified in smoking- and asthma-related microarray data sets and an epidemiologic study significantly reduced the risk of asthma. PMID: 26932165
The expression of elafin in fallopian tubes of ectopic pregnancies is reduced. PMID: 25356943
The elevations in cervical elafin and SLPI expression in the women with preterm delivery might reflect the local response to the pathogen invasion into the cervix preceding preterm labor. PMID: 25559229
PI3 expression was downregulated in 24% of DCIS and 83% of invasive breast tumors. PI3 was also downregulated in 33% of ovarian cystadenomas, 43% of borderline ovarian tumors, and 86% of invasive ovarian carcinomas. PMID: 25551582
The imbalance between neutrophil elastase and its inhibitors, such as elafin, presents an important therapeutic target in breast cancer. PMID: 25195861
Higher urinary elafin levels are associated with an increased risk of micro- and macroalbuminuria, acute kidney injury and chronic kidney disease, and death after hematopoietic stem cell transplantation. PMID: 25388519
cutaneous elafin does not seem to discriminate acute graft-versus-host disease from drug hypersensitivity rash lesions, but there may be an association between cutaneous elafin expression and poor prognosis for patients with cutaneous GVHD PMID: 25405322
Data indicate that elafin overexpression is associated with poor overall survival. PMID: 24469047
Low expression of elafin gene correlated with significantly reduced time to relapse, and when combined with high expression of elastase gene was associated with decreased survival in breast cancer patients PMID: 23320734
Elafin has important roles in normal homoeostasis and at sites of inflammation. These roles include antiprotease and antimicrobial activity. PMID: 19906197
Results confirm the association between SNP rs2664581 and enhanced risk of developing acute respiratory distress syndrome. PMID: 24617927
Tubal epithelium from women with hydrosalpinx has lower levels of elafin expression. PMID: 23885101
Elafin expression in the small intestinal epithelium was lower in patients with active celiac disease compared with control patients. In vitro, elafin significantly slowed the kinetics of the deamidation of the 33-mer peptide to its more immunogenic form. PMID: 24710505
Production of HBD2 and elafin by vaginal epithelial cells is sensitive to E, suggesting that innate immune protection varies in the vagina across the menstrual cycle. PMID: 23398087
Elafin expression may establish the diagnosis of pre-engraftment syndrome and graft versus host disease. PMID: 23866880
Trappin-2 and elafin significantly reduce HSV-2 viral attachment to, and replication in genital epithelial cells. PMID: 23637403
The N-terminus of elafin is critical for intranuclear localization and anti-HIV-1 activity. PMID: 23300756
Studies indicate that Trappin-2/Elafin possessing anti-microbial properties against different classes of pathogens including viruses, fungi and bacteria. PMID: 22634606
The results suggest that decreased expression of elastin and LOX and increased expression of elafin in the cardinal ligaments may contribute to pelvic organ prolapse. PMID: 19087518
The endometrium of women with hydrosalpinx has an increased number of neutrophils and lower expression of elafin, an elastase inhibitor and natural antimicrobial molecule. PMID: 21392745
these results collectively suggest that, in melanoma cells, Foxa2 expression is silenced and therefore elafin is maintained unexpressed to facilitate cell proliferation in the disease melanoma. PMID: 21466784
ability to modulate the expression of some P.aeruginosa virulence factors PMID: 20932308
These results suggest that elafin can maintain airway epithelium integrity by protecting airway epithelial cells and enhancing the anti-inflammatory capability of airway. PMID: 19823954
increased production at the secretory phase of the menstrual cycle relative to the proliferative phase PMID: 19824918
Elafin has a role in graft-versus-host disease of the skin PMID: 20371463
Elafin may serve as a determinant of poor survival in serous ovarian carcinomas PMID: 20126474
Overexpression of elafin in epithelial cells attenuated the damage of Pseudomonas aeruginosa biofilm. PMID: 19420899
Elafin is a component of cervicovaginal secretions in pregnancy, and levels are diminished in bacterial vaginosis. PMID: 19723838
Synthesized locally at mucosal sites. Efficiently inhibit target enzymes released into interstitium. Might be important in controlling excessive neutrophil elastase release in chronic obstructive pulmonary disease and cystic fibrosis. (review) PMID: 11667971
regulation of the elafin gene is dependent on chromatin structure in keratinocytes PMID: 12542536
results indicate that elafin may have a dual function, promoting up-regulation of local lung innate immunity while simultaneously down-regulating potentially unwanted systemic inflammatory responses in the circulation PMID: 12819058
elafin regulates proteolytic enzymes during menstruation and contributes to the innate defense against uterine infection PMID: 12970320
Serine elastase inhibition (elafin overexpression) appears to suppress inflammation, cardiac dilatation, and dysfunction after myocardial infarct. PMID: 14693682
Gene augmentation of human elafin suggests a novel and extended anti-inflammatory role for this human neutrophil elastase inhibitor working as an effector of innate immunity to protect tissues against maladaptive inflammatory responses. PMID: 15034071
The differential biological activity of elafin in different conditions suggests a role for this molecule in either LPS detoxification or activation of innate immune responses, depending on the external cellular environment. PMID: 15668324
Oxidized elafin poorly inhibits the elastolytic activity of leukocyte elastase and proteinase 3. PMID: 16279952
transglutaminase 2-mediated cross-linking of serine protease inhibitors elafin and trappin-2 to neutrophil serine proteinases preserves their inhibitory capacities PMID: 16300411
The pleiotropic molecule elafin has significant potential in modulating antigen-presenting cell numbers and activity, and could be beneficial in mucosal protective strategies. PMID: 16424380
The promoter sequences of PI3 have a high degree of variability. PMID: 16719916
beneficial effects of pre-elafin could be mediated, at least in part, by its ability to increase G-CSF levels in the lung PMID: 16913840
The transglutaminase substrate moiety of elafin plays an important role in anchoring elafin at its proper sites of action during UV-induced aging processes. PMID: 17139263
Levels of mRNA and immunostaining of the antiproteases elafin and SLPI were enhanced strongly in inflamed versus noninflamed UC. Elafin and SLPI may be added to the list of defensin-like peptides with diminished induction in CD versus UC. PMID: 17200145
full antipeptidase activity of pre-elafin is essential to protect against lung tissue lesions PMID: 17489739
This review focuses on recent findings revealing that elafin has many biological functions as diverse as anti-bacterial, anti-fungal, anti-viral, anti-inflammatory and immuno-modulatory functions. PMID: 17964057
We report here that pre-elafin inhibits a secreted peptidase to prevent Pseudomonas aeruginosa growth in vitro. PMID: 18025118