Recombinant Human Evolutionarily conserved signaling intermediate in Toll pathway, mitochondrial (ECSIT)

Code CSB-YP859414HU
MSDS
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Source Yeast
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Code CSB-EP859414HU
MSDS
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Source E.coli
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Code CSB-EP859414HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP859414HU
MSDS
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Source Baculovirus
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Code CSB-MP859414HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ECSIT
Uniprot No.
Alternative Names
Ecsit; ECSIT homolog; ECSIT protein; ECSIT_HUMAN; Evolutionarily conserved signaling intermediate in Toll pathway; mitochondrial; Evolutionarily conserved signaling intermediate in Toll pathways; Likely ortholog of mouse signaling intermediate in Toll pathway evolutionarily conserved; Protein SITPEC; Signaling intermediate in Toll pathway evolutionarily conserved; SITPEC; SITPEC protein
Species
Homo sapiens (Human)
Expression Region
49-431
Target Protein Sequence
SS EQSLVPSPPE PRQRPTKALV PFEDLFGQAP GGERDKASFL QTVQKFAEHS VRKRGHIDFI YLALRKMREY GVERDLAVYN QLLNIFPKEV FRPRNIIQRI FVHYPRQQEC GIAVLEQMEN HGVMPNKETE FLLIQIFGRK SYPMLKLVRL KLWFPRFMNV NPFPVPRDLP QDPVELAMFG LRHMEPDLSA RVTIYQVPLP KDSTGAADPP QPHIVGIQSP DQQAALARHN PARPVFVEGP FSLWLRNKCV YYHILRADLL PPEEREVEET PEEWNLYYPM QLDLEYVRSG WDNYEFDINE VEEGPVFAMC MAGAHDQATM AKWIQGLQET NPTLAQIPVV FRLAGSTREL QTSSAGLEEP PLPEDHQEED DNLQRQQQGQ S
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Adapter protein of the Toll-like and IL-1 receptor signaling pathway that is involved in the activation of NF-kappa-B via MAP3K1. Promotes proteolytic activation of MAP3K1. Involved in the BMP signaling pathway. Required for normal embryonic development.; Required for efficient assembly of mitochondrial NADH:ubiquinone oxidoreductase.
Gene References into Functions
  1. mediates virus-triggered type I IFN induction by bridging RIG-I and MDA5 to the VISA complex PMID: 25228397
  2. Ubiquitination of ECSIT might have a role in the regulation of NF-kappaB activity in TLR4 signaling. PMID: 25355951
  3. Authors conclude that ECSIT appears to be a novel Hepatitis B virus X protein-interacting signal molecule and their interaction is mechanistically important in IL-1beta induction of NF-kappaB activation. PMID: 25449573
  4. Data show that the ECSIT (evolutionarily conserved signaling intermediate in Toll pathways) complex, including MEKK7 (TAK1) and TNF receptor-associated factor 6 (TRAF6), plays a role in Toll-like receptor 4 -mediated signals to activate NF-kappa B. PMID: 25371197
  5. TRIM59 interacts with ECSIT as an adaptor protein required for the TLR-mediated transduction pathway. PMID: 22588174

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Subcellular Location
Cytoplasm. Nucleus. Mitochondrion.
Protein Families
ECSIT family
Database Links

HGNC: 29548

OMIM: 608388

KEGG: hsa:51295

STRING: 9606.ENSP00000270517

UniGene: Hs.515146

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