Recombinant Human F-box only protein 44 (FBXO44)

Code CSB-YP872497HU
MSDS
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Source Yeast
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Code CSB-EP872497HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP872497HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP872497HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
FBXO44
Uniprot No.
Alternative Names
5730411K09; AV001623; DKFZp781J0852; F box gene 3 ; F box only protein 44 ; F box only protein 6a; F box protein 44 ; F box protein FBX30 ; F box/G domain protein 3; F-box only protein 44; F-box protein FBX30; F-box/G-domain protein 3; FBG3; FBX30; FBX44; FBX44_HUMAN; FBX6A; Fbxo44; Fbxo6a; MGC14140; RGD1562463
Species
Homo sapiens (Human)
Expression Region
1-255
Target Protein Sequence
MAVGNINELP ENILLELFTH VPARQLLLNC RLVCSLWRDL IDLVTLWKRK CLREGFITED WDQPVADWKI FYFLRSLHRN LLHNPCAEEG FEFWSLDVNG GDEWKVEDLS RDQRKEFPND QVKKYFVTSY YTCLKSQVVD LKAEGYWEEL MDTTRPDIEV KDWFAARPDC GSKYQLCVQL LSSAHAPLGT FQPDPATIQQ KSDAKWREVS HTFSNYPPGV RYIWFQHGGV DTHYWAGWYG PRVTNSSITI GPPLP
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
Gene References into Functions
  1. Although the overall structure of FBG3 is similar to that of Fbs1, the residues that form the Fbs1 carbohydrate-binding pocket failed to be superposed with the corresponding residues of FBG3. PMID: 26460611
  2. FBXO44-mediated degradation of RGS2 protein uniquely depends on a Cul4B/DDB1 complex. PMID: 25970626
  3. FBXO44 mediates BRCA1 ubiquitination and degradation PMID: 23086937
  4. Skp1 and Fbg3 were co-expressed in E. coli. The plate-shaped crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 34.1, b = 76.6, c = 193.9 A and one molecule per asymmetric unit. PMID: 20057081
Tissue Specificity
Abundantly expressed in brain and kidney. Expressed at lower levels in heart, spleen and liver.
Database Links

HGNC: 24847

OMIM: 609111

KEGG: hsa:93611

STRING: 9606.ENSP00000251547

UniGene: Hs.556006

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