Recombinant Human F-box/WD repeat-containing protein 2 (FBXW2)

Code CSB-YP891730HU
MSDS
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Source Yeast
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Code CSB-EP891730HU
MSDS
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Source E.coli
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Code CSB-EP891730HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP891730HU
MSDS
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Source Baculovirus
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Code CSB-MP891730HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
FBXW2
Uniprot No.
Alternative Names
F box and WD 40 domain containing protein 2; F box and WD repeat domain containing 2; F box and WD40 domain protein 2; F box/WD repeat containing protein 2; F-box and WD-40 domain-containing protein 2; F-box/WD repeat-containing protein 2; FBW 2; FBW2; FBXW 2; Fbxw2; FBXW2_HUMAN; Fwd2; Md6; Protein MD6
Species
Homo sapiens (Human)
Expression Region
1-454
Target Protein Sequence
MERKDFETWL DNISVTFLSL TDLQKNETLD HLISLSGAVQ LRHLSNNLET LLKRDFLKLL PLELSFYLLK WLDPQTLLTC CLVSKQWNKV ISACTEVWQT ACKNLGWQID DSVQDALHWK KVYLKAILRM KQLEDHEAFE TSSLIGHSAR VYALYYKDGL LCTGSDDLSA KLWDVSTGQC VYGIQTHTCA AVKFDEQKLV TGSFDNTVAC WEWSSGARTQ HFRGHTGAVF SVDYNDELDI LVSGSADFTV KVWALSAGTC LNTLTGHTEW VTKVVLQKCK VKSLLHSPGD YILLSADKYE IKIWPIGREI NCKCLKTLSV SEDRSICLQP RLHFDGKYIV CSSALGLYQW DFASYDILRV IKTPEIANLA LLGFGDIFAL LFDNRYLYIM DLRTESLISR WPLPEYRKSK RGSSFLAGEA SWLNGLDGHN DTGLVFATSM PDHSIHLVLW KEHG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
Gene References into Functions
  1. BTrCP-FBXW2-SKP2 axis forms an oncogene-tumour suppressor-oncogene cascade to control cancer cell growth with FBXW2 acting as a tumour suppressor by promoting SKP2 degradation. PMID: 28090088
  2. RACK1 competes with GCM1 for FBW2 and thereby prevents GCM1 ubiquitination, which is also supported by the observation that GCM1 is destabilized in RACK1-knockdown BeWo placental cells PMID: 23651062
  3. the SCF(hFBW2) E3 complex has a key role in targeting hGCMa to the ubiquitin-proteasome degradation system PMID: 15640526
  4. UBE2D2 is required for GCMa ubiquitination and for association with the SCF(FBXW2) complex. PMID: 18703417
Database Links

HGNC: 13608

OMIM: 609071

KEGG: hsa:26190

STRING: 9606.ENSP00000363036

UniGene: Hs.494985

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