Purity

≥ 95% as determined by SDS-PAGE.
≥ 95% as determined by SEC-HPLC.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized Human FGFR3 at 2 μg/mL can bind Anti-FGFR3 recombinant antibody (CSB-RA008646MA1HU). The EC50 is 3.364-3.827 ng/mL.

Target Names

FGFR3

Uniprot No.

P22607-2

Alternative Names

Fibroblast growth factor receptor 3; EC:2.7.10.1; FGFR-3; CD333; FGFR3; JTK4

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

127-377aa

Target Protein Sequence

DAPSSGDDEDGEDEAEDTGVDTGAPYWTRPERMDKKLLAVPAANTVRFRCPAAGNPTPSISWLKNGREFRGEHRIGGIKLRHQQWSLVMESVVPSDRGNYTCVVENKFGSIRQTYTLDVLERSPHRPILQAGLPANQTAVLGSDVEFHCKVYSDAQPHIQWLKHVEVNGSKVGPDGTPYVTVLKSWISESVEADVRLRLANVSERDGGEYLCRATNFIGVAEKAFWLSVHGPRAAEEELVEADEAGSVYAG

Mol. Weight

29.0 kDa

Protein Length

Partial of Isoform 2

Tag Info

C-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm sterile filtered PBS, 6% Trehalose, pH 7.4

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

FGFR3 dysregulation drives oncogenesis in bladder carcinoma, multiple myeloma, and skeletal dysplasia syndromes, making quantitative assessment of receptor-ligand and receptor-antibody interactions critical for therapeutic development. This mammalian-expressed construct spanning residues 127–377 of isoform 2 captures the extracellular ligand-binding domain with native glycosylation patterns that preserve conformational epitopes, as demonstrated by functional ELISA binding to an anti-FGFR3 recombinant antibody with an EC50 of 3.364–3.827 ng/mL. The validated binding activity supports use in competitive inhibition assays for blocking antibody screening, therapeutic antibody epitope mapping, and small-molecule inhibitor discovery campaigns targeting FGFR3-driven malignancies. Dual purity verification by SDS-PAGE and SEC-HPLC (both >95%) combined with endotoxin levels below 1.0 EU/μg meets the quality thresholds commonly required for surface plasmon resonance affinity characterization and biolayer interferometry kinetic studies.

Code	CSB-MP008646HU(F2)
Abbreviation	Recombinant Human FGFR3 protein, partial (Active)
MSDS	MSDS Datasheet
Size	$114
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized Human FGFR3 at 2 μg/ml can bind Anti-FGFR3 recombinant antibody (CSB-RA008646MA1HU). The EC₅₀ is 3.364-3.827 ng/mL. Biological Activity Assay The purity of FGFR3 was greater than 95% as determined by SEC-HPLC
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Recombinant Human Fibroblast growth factor receptor 3 (FGFR3), partial (Active)

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