Recombinant Human Filaggrin(FLG),partial

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Code CSB-YP008712HU
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names FLG
Uniprot No. P20930
Research Area Signal Transduction
Alternative Names ATOD2; Epidermal filaggrin; FILA_HUMAN; Filaggrin; Filaggrin precursor; Fillagrin; FLG; Profilaggrin
Species Homo sapiens (Human)
Source Yeast
Expression Region 3838-4061aa
Target Protein Sequence DSSRHSQSGQGESAGSRRSRRQGSSVSQDSDSEAYPEDSERRSESASRNHHGSSREQSRDGSRHPGSSHRDTASHVQSSPVQSDSSTAKEHGHFSSLSQDSAYHSGIQSRGSPHSSSSYHYQSEGTERQKGQSGLVWRHGSYGSADYDYGESGFRHSQHGSVSYNSNPVVFKERSDICKASAFGKDHPRYYATYINKDPGLCGHSSDISKQLGFSQSQRYYYYE
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 26.8kDa
Protein Length Partial
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Aggregates keratin intermediate filaments and promotes disulfide-bond formation among the intermediate filaments during terminal differentiation of mammalian epidermis.
Gene References into Functions
  1. The present study demonstrated that the downregulation of filaggrin in the epidermis by toluene is mediated by ERK1/2 and STAT3-dependent pathways. PMID: 27498358
  2. FLG mutation was not associated with MM in the studied populations. PMID: 28833578
  3. The results show that patients carrying filaggrin mutations had increase prevalence of Staphylococcus aureus colonization. PMID: 28317091
  4. We suggest that SNP in FLG (rs11204981) may serve as an important predictive marker for the combined eczema plus asthma phenotype, and that the highest level of expression in heterozygous may have a protective role in developing allergy phenotype. PMID: 29569866
  5. This study indicates an increased susceptibility to actinic keratosis in individuals with homozygous, but not heterozygous, FLG mutations and in patients with atopic dermatitis compared to psoriasis. PMID: 28213896
  6. This study demonstrated, for the first time, that FLG expression in UCB is associated with eczema development in infancy. Moreover, our analysis provided prediction models that were capable of discriminating, to a great extent, between those who will and will not develop eczema in infancy. PMID: 28502108
  7. In a side-to-side comparison of two different methods to determine NMF in atopic dermatitis patients: Raman microspectroscopy and stratum corneum tape stripping followed by HPLC. both methods demonstrated a concentration-depth dependence of NMF and reduced NMF levels in the carriers of filaggrin null mutations PMID: 27805415
  8. FLG and POSTN expression may be downregulated and upregulated, respectively, in the esophageal mucosa of patients with active eosinophilic esophagitis, and these changes may be restored with treatment in a significant percentage of cases. PMID: 28644349
  9. immunoreactivity for filaggrin was significantly more intense in the oral mucosa in the patients with OLP/OLL compared with healthy controls PMID: 28000306
  10. atopic dermatitis patients without palmoplantar hyperlinearity unlikely to carry FLG mutations PMID: 27120251
  11. FLG mutations are strongly associated with atopic eczema and confer a significant risk of allergic sensitization and asthma in the context of eczema in Polish children. PMID: 29068602
  12. There was no association of the atopy risk variants in the FLG gene with OFG. PMID: 27306066
  13. suggest that FLG P478S is a kind of disease modifier which affects serologic parameters such as EDN and ECP PMID: 29281699
  14. In this study of 97 174 individuals from the Danish general population, FLG loss-of-function mutations were associated with increased ischemic stroke risk PMID: 28164424
  15. study found that among patients with atopic dermatitis (AD), common FLG null mutations are associated with earlier AD onset in a dose-dependent manner, whereas TSLP rs1898671 appears unrelated to the timing of AD onset PMID: 28479194
  16. The two SNPs K4671X and rs3126085 of FLG were related to Epstein-Barr virus (EBV)-associated gastric carcinoma. PMID: 28455573
  17. FLG mutation is associated with IgE sensitization to peanut but not to other allergens in Swedish children up to 16 years of age PMID: 28456621
  18. Erythemal doses of ultraviolet B exert acute effects on profilaggrin mRNA and filaggrin protein in human skin in vivo. PMID: 28358172
  19. FLG mutations are associated with early onset of atopic dermatitis, more severe clinical course of disease, and a significantly increased risk ofMolluscum contagiosum sustained skin infection PMID: 28866311
  20. Study conducted in Croatia found a low frequency of FLG null-mutations in general population (2.6%) and did not confirm FLG null-mutations as an etiological factor for Atopy and Atopic disease in the studied population. PMID: 29087092
  21. women with FLG mutations may have an increased risk of AD flares during pregnancy and of enduring postpartum problems attributable to perineal trauma during delivery. PMID: 26835886
  22. FLG mutations are risk factors for atopic dermatitis in Finns, but disease severity and treatment response were independent of patient FLG status. PMID: 27840886
  23. Patients with both atopic dermatitis and common filaggrin gene mutations are more frequently affected by reduced health-related quality of life PMID: 27995642
  24. Multiple lines of evidence suggest that FLG genetic variation, have little or no effect on fitness in modern humans. Haplotype-level analysis revealed a recent selective sweep which increased the allele frequency of the Huxian haplogroup in Asian populations. PMID: 27678121
  25. FLG mutations lead to alterations in epidermal eicosanoid metabolism in atopic dermatitis patients PMID: 27793761
  26. Filaggrin gene mutations are risk factors for the presence and persistence of atopic dermatitis and explain the discordance of atopic dermatitis within dizygotic twin pairs. PMID: 27653621
  27. Variations in FLG and TSLP genotype were associated with differences in self-reported skin clearance, TCI usage, and steroid usage. PMID: 27902816
  28. We show that subjects with FLG-null mutations have more mature Langerhans cells in nonlesional skin irrespective of whether they have AD. PMID: 26934939
  29. we have proposed that this latitude-dependent gradient of FLG mutations across Europe, Asia and Africa could have provided an evolutionary advantage for heterozygous FLG mutation carriers, residing at northern latitudes, depletion of the FLG downstream product, trans-urocanic acid, would facilitate the intracutaneous synthesis of vitamin D3 by allowing increased transcutaneous absorption of UVB photons PMID: 28338939
  30. The results of the present study demonstrate that filaggrin knockdown inhibits NHEK migration, adhesion and proliferation, promotes apoptosis and disturbs cell cycle progression. PMID: 27485743
  31. FLG rs2065955 polymorphism was significantly related to gastric carcinoma. PMID: 27535066
  32. Four FLG null mutations (3321delA, K4022X, S3296X, and S2889X) were identified in Korean patients with Atopic Dermatitis PMID: 28120571
  33. IL-33, which is a representative Th2 cytokine, affect as a crucial factor of skin barrier dysfunction by reducing FLG expression in human keratinocytes. PMID: 26867960
  34. The authors data reveals that the CNV of FLG is associated with AD development in Koreans. PMID: 26554544
  35. Filaggrin Gene Mutation c.3321delA is Associated with Dry Phenotypes of Atopic Dermatitis in the Chinese Han Population PMID: 27270549
  36. A mutation p.Y1767X, was related to early onset and severe symptoms, which last until adulthood, and severe atopic dermatitis with other atopic diseases, such as asthma. PMID: 27366014
  37. The present study examined the possible relationship between SNPs of FLG and chronic idiopathic urticaria (CIU) for the first time, and demonstrated that none of five investigated SNPs (rs2485518, rs3126065, rs2786680, rs3814300, and rs3814299) are correlated with CIU in an Iranian population. PMID: 26796858
  38. The study revealed 66 FLG mutation carriers and demonstrated an association between c.2282del4 deletion and atopic dermatitis development in Russians and Tatars of Volga-Ural region of Russia. PMID: 27363669
  39. The levels of filaggrin, inflammatory T helper 2 polarizing cytokines (thymic stromal lymphopoietin (TSLP) and interleukin 33 (IL-33)) and chemokine (C-C motif) ligand 27 (CCL27), histological severity markers, T and dendritic cell counts in biopsies from lesional skin of severe atopic dermatitis patients with and without filaggrin mutation and healthy skin were quantified. No significant differences were found. PMID: 26536977
  40. The imbalance between Th1 and Th2 polarized immune response seems to extend to Filaggrin homeostasis, through the network of Filaggrin processing enzymes. PMID: 26831231
  41. Levels of FLG, FLG2 and SPRR3 mRNAs and proteins were reduced in AD skin. PMID: 27304082
  42. Data suggest that the c.1360A>G (p.T454A) and c.10363G>T (p.D3455Y) mutations of the filaggrin (FLG) gene may lead to alteration of the structure and function of the FLG protein and cause ichthyosis vulgaris in the two families. PMID: 27577213
  43. genotype-phenotype correlation not obvious in Korean atopic dermatitis patients with null mutations PMID: 25997159
  44. Results show a strong association of FLG loss-of-function mutations was found with doctor-diagnosed CD and to a lesser extent also with self-reported CD, but not with respiratory symptoms or atopy. PMID: 26451970
  45. Identify acefylline as an activator of peptidylarginine deiminase 1 and 3 in the epidermis, resulting in filaggrin deimination. PMID: 26616205
  46. FLG has been, and still remains the major susceptibility gene in patients with AD. PMID: 25580797
  47. Letter: Knockdown of either filaggrin or loricrin increases the productions of interleukin (IL)-1alpha, IL-8, IL-18 and granulocyte macrophage colony-stimulating factor in stratified human keratinocytes. PMID: 26381575
  48. Supplementation with the PPARalpha agonist WY14643 improved the homeostasis and barrier function of filaggrin deficient skin models by normalization of the free fatty acid profile. PMID: 26472199
  49. Filaggrin genotype does not determine the skin's threshold to UV-induced erythema. PMID: 26830116
  50. In Iranian patients, this study demonstrated that there was no significant association between polymorphisms of FLG gene variants and AD. PMID: 25230061
  51. Filaggrin expression was present in healthy skin PMID: 25776938
  52. Our findings indicate an association between xerosis and asthma in men independent of atopic dermatitis and FLG mutations. PMID: 25712346
  53. Results show that among participants aged over 60 years at baseline, we found statistically significant lower risks of all cancers and NMSC among FLG mutation carriers. PMID: 24628370
  54. Filaggrin breakdown products determine corneocyte conformation in patients with atopic dermatitis PMID: 26071937
  55. Filaggrin polymorphism Pro478Ser Is associated with increased disease severity and staphylococcus aureus colonization in atopic dermatitis patients. PMID: 27012026
  56. Results suggest that skin reaction and regeneration response was more rapid amongst individuals with AD who carried the mutation for FLG PMID: 25581911
  57. These results indicate that FLG mutations might be involved in the pathogenesis of wheat-dependent exercise-induced anaphylaxis in the family studied. PMID: 24629053
  58. the 2282del4 mutation was associated only with atopic dermatitis (AD) that developed during infancy or in early childhood, not with AD development in late childhood or adulthood; similar associations also observed for the combined 2282del4 or R501X genotype PMID: 25314673
  59. FLG loss-of-function mutations were associated with higher incidence of HPV-related cancers and pre-cancers that are potentially screening and vaccine preventable. PMID: 24905740
  60. Loss-of-function variants of the filaggrin gene are associated with clinical reactivity to foods PMID: 25620092
  61. Study investigated the 4 most prevalent European FLG mutations (c.2282del4, p.R501X, p.R2447X, and p.S3247X) in two samples including 759 and 450 atopic dermatitis families and found that overall, children of FLG-carrier mothers had a 1.5-fold increased AD risk (S1 = 1.50, Pmeta-analysis = 8.4 x 10-8). PMID: 25757221
  62. Thirty-five percent of keratosis pilaris patients displayed filaggrin mutations, demonstrating that filaggrin mutations only partially account for the KP phenotype. PMID: 25660180
  63. Filaggrin null mutation and sunlight exposure is associated in the development of atopic eczema. PMID: 26282804
  64. TSLP downregulates filaggrin expression in human skin keratinocytes PMID: 26026341
  65. Loss of mutation of filaggrin is not associated with chronic actinic dermatitis. PMID: 25734812
  66. There was no association between FLG mutations and food allergen and aeroallergen sensitization, respectively PMID: 25678087
  67. IL-33 increases the skin barrier permeability to allergens by downregulating the expression of filaggrin in atopic dermatitis. PMID: 25863977
  68. Filaggrin mutations in a Western siberian population and their association with atopic dermatitis in children PMID: 25390410
  69. We sought to explain the associated severe nonlesional skin dryness in our African American children with atopic dermatitis by genotyping a previously described European dose-dependent risk factor for AD, intragenic FLG repeats, or CNV. PMID: 25564772
  70. no evidence of correlation between gene expression and promoter methylation in skin and buccal samples PMID: 24912553
  71. this is the first study to our knowledge to assess whether FLG mutations are associated with chronic rhinosinusitis and we found no evidence of an association in our series of patients PMID: 25747786
  72. Carriage of FLG mutations was not associated with the risk of cervical cancer. PMID: 25383447
  73. The FLG loss-of-function mutation frequency was not significantly different between the atopic dermatitis and non-AD groups in a cohort of children from Ishigaki Island PMID: 25086748
  74. study indicates that the filaggrin gene mutation c.3321delA is associated with clinical phenotypes of atopic dermatitis in the Chinese Han population PMID: 24858702
  75. The coexistence of allergic disorders is frequent, and allergic sensitization and FLG variants jointly increased risk of allergic comorbidities, which may represent more severe and complex clinical phenotypes. PMID: 24708301
  76. data demonstrate a prevalence of filaggrin mutations in the African American population that exceeds previously published data, although the overall prevalence is still lower than in other populations PMID: 24920311
  77. It is an essential skin barrier protein. PMID: 25816564
  78. Filaggrin mutations affected the lifetime prevalence of hand and foot dermatitis in participants with a history of AD PMID: 25659224
  79. genetic polymorphism is associated with asthma in Taiwanese children with mite allergy PMID: 25528737
  80. The results of the present study suggest that the FLG P478S polymorphism alone and combined with other factors influences FFA levels and increases the susceptibility to atopic dermatitis PMID: 24521637
  81. FLG mutations are common in a US group of white patients with severe atopic dermatitis PMID: 24565632
  82. Knockdown of filaggrin in a three-dimensional reconstructed human epidermis impairs keratinocyte differentiation. PMID: 24940654
  83. this article reviews the various causes of low filaggrin levels, centralizing the functional and morphologic role of a deficiency in filaggrin, its metabolites, or both in the etiopathogenesis of atopic dermatitis. [review] PMID: 25065719
  84. Early-life environmental peanut exposure is loss-of-function mutation is associated with an increased risk of peanut sensitization and allergy in children PMID: 25282568
  85. loss-of-function mutations are associated with food allergies in older children through eczema and food allergen sensitization during early childhood PMID: 25174864
  86. lack of significant diversity in skin pH in relation to filaggrin mutation carrier status suggests that the effect of filaggrin mutations on skin pH i PMID: 24819286
  87. Allergic sensitization and eczema modulated the association between FLG variants and asthma but not rhinitis. PMID: 25277085
  88. Results suggest that patients with FLG mutant-type AD may have a higher risk of allergic sensitization compared with patients with the wild-type. PMID: 24251354
  89. Extracellular space and lipid metabolism are important in atopic skin pathology independent of FLG genotype, whereas an aberrant defense response is seen in subjects with FLG mutations. PMID: 24880632
  90. Increased efficacy of omalizumab in atopic dermatitis patients with wild-type filaggrin status and higher serum levels of phosphatidylcholines. PMID: 24111531
  91. In this study, an increased risk of hand eczema conferred by FLG mutations could not be shown, but subjects with concomitant FLG mutations and atopic dermatitis showed the highest risk of hand eczema during traineeships. PMID: 24102300
  92. Sensitization for type I allergens did not differ between FLG loss-of-function mutation carriers and wild-type subjects. For type IV allergens, more FLG loss-of-function carriers were sensitized to lanolin and p-tert-butylphenol-formaldehyde resin. PMID: 23848345
  93. Filaggrin is overexpressed in lesional chronic idiopathic urticaria (CIU) skin, and increased filaggrin expression is positively correlated with urticaria severity in CIU. PMID: 24726196
  94. These findings suggest that filaggrin-2 may play an overlapping role with filaggrin in epithelial cornification; however, it may also have a partially distinct role in the molecular processes of cornification. PMID: 24813994
  95. Study investigated the role of filigrin homozygous mutations in basal cell carcinoma and found no evidence of elevated risk. PMID: 23927042
  96. A complete, but not a partial filaggrin deficiency is associated with moderate changes in TEWL and skin hydration, revealing surprisingly only a mild disturbance of the epidermal permeability barrier function PMID: 23297869
  97. Filaggrin was detectable in a subfraction of oral squamous carcinomas and was restricted to keratinising areas of the carcinomas PMID: 23645350
  98. Filaggrin is a predominant member of the denaturation-resistant nickel-binding proteome of human epidermis PMID: 24157460
  99. Diminished filaggrin mutation found in patients diagnosed with chronic atopic dermatitis. PMID: 24401911
  100. The interplay of the UCA/PCA and the sPLA2/NHE-1 acidification pathways of the skin and the impact of FLG insufficiency on skin lipid composition and organization in reconstructed skin are described. PMID: 24061166

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Involvement in disease Ichthyosis vulgaris (VI); Dermatitis atopic 2 (ATOD2)
Subcellular Location Cytoplasmic granule
Protein Families S100-fused protein family
Tissue Specificity Expressed in skin, thymus, stomach, tonsils, testis, placenta, kidney, pancreas, mammary gland, bladder, thyroid, salivary gland and trachea, but not detected in heart, brain, liver, lung, bone marrow, small intestine, spleen, prostate, colon, or adrenal
Database Links

HGNC: 3748

OMIM: 135940

KEGG: hsa:2312

STRING: 9606.ENSP00000357789

UniGene: Hs.654510

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