Recombinant Human Flt3-interacting zinc finger protein 1 (FIZ1)

In Stock
Code CSB-EP839415HU
MSDS
Size $256
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
FIZ1
Uniprot No.
Research Area
others
Alternative Names
Zinc finger protein 798
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
1-496aa
Target Protein Sequence
MDDVPAPTPAPAPPAAAAPRVPFHCSECGKSFRYRSDLRRHFARHTALKPHACPRCGKGFKHSFNLANHLRSHTGERPYRCSACPKGFRDSTGLLHHQVVHTGEKPYCCLVCELRFSSRSSLGRHLKRQHRGVLPSPLQPGPGLPALSAPCSVCCNVGPCSVCGGSGAGGGEGPEGAGAGLGSWGLAEAAAAAAASLPPFACGACARRFDHGRELAAHWAAHTDVKPFKCPRCERDFNAPALLERHKLTHDLQGPGAPPAQAWAAGPGAGPETAGEGTAAEAGDAPLASDRRLLLGPAGGGVPKLGGLLPEGGGEAPAPAAAAEPSEDTLYQCDCGTFFASAAALASHLEAHSGPATYGCGHCGALYAALAALEEHRRVSHGEGGGEEAATAAREREPASGEPPSGSGRGKKIFGCSECEKLFRSPRDLERHVLVHTGEKPFPCLECGKFFRHECYLKRHRLLHGTERPFPCHICGKGFITLSNLSRHLKLHRGMD
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
58.0 kDa
Protein Length
Full Length
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
May be a transcriptional repressor of NRL function in photoreceptors. Does not repress CRX-mediated transactivation.
Gene References into Functions
  1. Signal transducer/transcription factor FIZ1 regulated signaling pathways are implicated in regulation of the balance between proliferation and differentiation in keratinocytes. PMID: 24956037
  2. Activators of the Rhodopsin gene are present before activation of the gene. FIZ1 expression rises at this time and it can synergize with NRL and CRX at the Rho and PDE6B promoters. FIZ1 may syncronize the onset of maturation related gene expression. PMID: 17141759
  3. Homologous to mouse and bovine Fiz1 (flt-3 interacting zinc finger). Interacts with NRL (neural retina leucine zipper). Antibody detects protein in human, mouse, bovine retina. Possible role for Fiz1 as a transcriptional repressor. PMID: 12566383
Subcellular Location
Cytoplasm. Nucleus.
Tissue Specificity
Widely expressed.
Database Links

HGNC: 25917

OMIM: 609133

KEGG: hsa:84922

STRING: 9606.ENSP00000221665

UniGene: Hs.515617

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