Recombinant Human G-protein-signaling modulator 3 (GPSM3)

Code CSB-YP896517HU
MSDS
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Source Yeast
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Code CSB-EP896517HU
MSDS
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Source E.coli
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Code CSB-EP896517HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896517HU
MSDS
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Source Baculovirus
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Code CSB-MP896517HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GPSM3
Uniprot No.
Alternative Names
Activator of G-protein signaling 4; AGS4; C6orf9; G protein signalling modulator 3 (AGS3 like; C. elegans); G-protein signaling modulator 3 (AGS3-like; C. elegans); G-protein-signaling modulator 3; G18; G18.1a; G18.1b; G18.2; Gpsm3; GPSM3_HUMAN; NG1; OTTHUMP00000029351; Protein G18
Species
Homo sapiens (Human)
Expression Region
1-160
Target Protein Sequence
MEAERPQEEE DGEQGPPQDE EGWPPPNSTT RPWRSAPPSP PPPGTRHTAL GPRSASLLSL QTELLLDLVA EAQSRRLEEQ RATFYTPQNP SSLAPAPLRP LEDREQLYST ILSHQCQRME AQRSEPPLPP GGQELLELLL RVQGGGRMEE QRSRPPTHTC
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Interacts with subunit of G(i) alpha proteins and regulates the activation of G(i) alpha proteins.
Gene References into Functions
  1. role for GPSM3 in neutrophil migration toward proinflammatory chemoattractants LTB4 and CXCL8-a discovery that helps strengthen the association between the promoter-weakening GPSM3 SNP rs204989 and a reduced risk of rheumatoid arthritis PMID: 27307211
  2. Genetic variations in GPSM3 which are associated with protection from rheumatoid arthritis affect its transcript abundance. PMID: 26821282
  3. findings suggest that GPSM3 acts as a direct negative regulator of NLRP3 function. PMID: 25271165
  4. Phosphorylation of GPSM3 by a proline-directed serine/threonine kinase and the resultant association of 14-3-3 is the first description of post-translational regulation of GPSM3 subcellular localization PMID: 22843681
  5. GPSM3 increases Gbeta stability until formation of the Gbetagamma dimer, including association of the Gbeta-GPSM3 complex with phosducin-like protein PhLP and T-complex protein 1 subunit eta (CCT7), two known chaperones of neosynthesized Gbeta subunits. PMID: 22167191
  6. AGS4-Galpha(i) complexes directly couple to a G-protein-coupled receptor and may serve as substrates for agonist-induced G-protein activation. PMID: 20452976
  7. Mutational analysis of the role of GoLoco motifs in guanine nucleotide dissociation inhibitor activity of G18 protein. PMID: 14656218

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Subcellular Location
Cytoplasm.
Tissue Specificity
Expressed in heart, placenta, lung and liver.
Database Links

HGNC: 13945

KEGG: hsa:63940

STRING: 9606.ENSP00000364180

UniGene: Hs.520046

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