Recombinant Human GTP-binding protein GEM (GEM)

Code CSB-YP009362HU
MSDS
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Source Yeast
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Code CSB-EP009362HU
MSDS
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Source E.coli
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Code CSB-EP009362HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP009362HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP009362HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GEM
Uniprot No.
Alternative Names
GEM; KIRGTP-binding protein GEM; GTP-binding mitogen-induced T-cell protein; RAS-like protein KIR
Species
Homo sapiens (Human)
Expression Region
1-296
Target Protein Sequence
MTLNNVTMRQ GTVGMQPQQQ RWSIPADGRH LMVQKEPHQY SHRNRHSATP EDHCRRSWSS DSTDSVISSE SGNTYYRVVL IGEQGVGKST LANIFAGVHD SMDSDCEVLG EDTYERTLMV DGESATIILL DMWENKGENE WLHDHCMQVG DAYLIVYSIT DRASFEKASE LRIQLRRARQ TEDIPIILVG NKSDLVRCRE VSVSEGRACA VVFDCKFIET SAAVQHNVKE LFEGIVRQVR LRRDSKEKNE RRLAYQKRKE SMPRKARRFW GKIVAKNNKN MAFKLKSKSC HDLSVL
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Could be a regulatory protein, possibly participating in receptor-mediated signal transduction at the plasma membrane. Has guanine nucleotide-binding activity but undetectable intrinsic GTPase activity.
Gene References into Functions
  1. Down regulation of Gem expression was involved in the pathogenesis of hepatocellular carcinoma (HCC), and it might be a favorable independent prognostic parameter for HCC. PMID: 25155751
  2. These findings demonstrate a new role of Gem/Gmip/RhoA signaling in cortical actin regulation during early mitotic stages. PMID: 25173885
  3. Gem is involved both in T-cell spontaneous cell migration as well as chemotaxis and plays an important role in cell-to-cell HTLV-1 viral transmission. PMID: 24586148
  4. Data show a mechanism by which GTPase Gem contributes to the mitotic progression by maintaining correct spindle length through the kinesin Kif9. PMID: 22964304
  5. Gem contains two candidate inhibitory sites, each capable of producing full inhibition of P/Q-type Ca(2+) channels. PMID: 22589533
  6. GEM plays a key role during C. pneumoniae entry into human cells. PMID: 20233927
  7. We discuss the structural and functional properties of RGK proteins and highlight recent work addressing the mechanism of Gem regulation by calmodulin and 14-3-3. PMID: 16242932
  8. crystallographic structure and biochemical characterization indicate that Gem G-domain markedly prefers GDP over GTP PMID: 17052716
  9. Gem is a bona fide GTPase that exhibits striking structural and biochemical features that should impact its regulation and cellular activities PMID: 17107948

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Subcellular Location
Cell membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families
Small GTPase superfamily, RGK family
Tissue Specificity
Most abundant in thymus, spleen, kidney, lung, and testis. Less abundant in heart, brain, liver and skeletal muscle.
Database Links

HGNC: 4234

OMIM: 600164

KEGG: hsa:2669

STRING: 9606.ENSP00000297596

UniGene: Hs.654463

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